LOCUS       Exported                1004 bp ds-DNA     linear   SYN 12-JUN-2021
DEFINITION  HO-TEF1p-ATeam1.03-KanMX4-HO. Codon optimized for yeast version of 
            the ATeam1.03 ATP FRET sensor (originally from Imamura et al. PNAS, 
            2009 ), expressed via the TEF1 promoter. HO locus integration..
ACCESSION   .
VERSION     .
KEYWORDS    pTEF:ATP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Papagiannakis A, Niebel B, Wit EC, Heinemann M
  TITLE     Autonomous Metabolic Oscillations Robustly Gate the Early and Late 
            Cell Cycle.
  JOURNAL   Mol Cell. 2017 Jan 19;65(2):285-295. doi: 
            10.1016/j.molcel.2016.11.018. Epub 2016 Dec 15.
  PUBMED    27989441
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
ORIGIN
        1 accggtagtg catatcaatt tcaaagtcaa tttaccgtat gttgcgtcac cttcaccttc
       61 accactaact gaaaacttat gaccattgac gtcaccgtct aattctacta aaatagggac
      121 gacacctgtg aataattctt cacctttact aaccatacca ccagaaccac ccttgtataa
      181 ttcatccata cccaaagtaa taccggcagc ggtgacaaat tctaataaaa ccatatgatc
      241 tcttttttcg ttaggatctt tggacaactt agattggtag gacaaatagt gattatcagg
      301 taataataca ggaccgtcac cgataggtgt gttttgttga tagtggtctg ctaattgaac
      361 accaccgtcc atgaattcgt tagccttacc agcaacatcc aatctgttca aagctctttg
      421 taaagccaat tctgctcttc tgatatcggt atcatcagat tgagagttca atctttcttg
      481 ggctctttgt ctagcagctt cagctctttc tttgtcaata ccttcagctg tttcagcagc
      541 ttgagcgtta atagtaacat gatctggtct aacttcaaca gtaccacctg aaactgcaac
      601 catttctgtt tgaccgtcct ttttcaatct aacggcacca attttcaatg gggcttttgt
      661 aggaatatga cctggcaaaa tacccaaatc accagattca gctctgacag aaaccatttc
      721 aatatcggca tcgtaaacag gaccatctgg agtagtaatg ttaaccttaa cggtcttcat
      781 atcgatagca gcagtaacaa attccaacaa gaccatgtga tctctttttt cgtttgggtc
      841 cttagacaat ttggattggg tagacaagta atggttatct ggcaacaaaa ctggaccatc
      901 accaattgga gtgttttgtt ggtaatgatc agccaattgt acaccaccat cttcgatgtt
      961 gtgtctgatc ttgaaatgag ccttaatacc gttcttttgc ttat
//
