LOCUS       Exported                1072 bp ds-DNA     linear   SYN 12-JUN-2021
DEFINITION  HO-TEF1p-ATeam1.03-KanMX4-HO. Codon optimized for yeast version of 
            the ATeam1.03 ATP FRET sensor (originally from Imamura et al. PNAS, 
            2009 ), expressed via the TEF1 promoter. HO locus integration..
ACCESSION   .
VERSION     .
KEYWORDS    pTEF:ATP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Papagiannakis A, Niebel B, Wit EC, Heinemann M
  TITLE     Autonomous Metabolic Oscillations Robustly Gate the Early and Late 
            Cell Cycle.
  JOURNAL   Mol Cell. 2017 Jan 19;65(2):285-295. doi: 
            10.1016/j.molcel.2016.11.018. Epub 2016 Dec 15.
  PUBMED    27989441
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      502..699
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 gcgtaatgcc caatttttcg cctttgtctt ttgccttttt cacttcacgt gcttctggta
       61 catacttgca atttatacag tgatgaccgc tgaatttgta tcttccatag catctagcac
      121 atactcgatt tttaccactc caatctttat aaaaatactt gattcccttt ctgggacaag
      181 caacacagtg ttttagattc tttttttgtg atattttatg ctgttctccc acacagcagc
      241 ctcgacatga tttcacttct attttgttgc caagcaagaa atttttatgg ccttctatcg
      301 taagcccata tacagtactc tcaccctgga aatcatccgt gaagctgaaa tatacgggtt
      361 ccctttttat aattggcgga acttctcttg ttttgtgacc acttcgacaa tatgacaaaa
      421 cattctgtga agttgttccc ccaggaatta attcgagctc gttttcgaca ctggatggcg
      481 gcgttagtat cgaatcgaca gcagtatagc gaccagcatt cacatacgat tgacgcatga
      541 tattactttc tgcgcactta acttcgcatc tgggcagatg atgtcgaggc gaaaaaaaat
      601 ataaatcacg ctaacatttg attaaaatag aacaactaca atataaaaaa actatacaaa
      661 tgacaagttc ttgaaaacaa gaatcttttt attgtcagta ctgattagaa aaactcatcg
      721 agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa
      781 agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc
      841 tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg
      901 tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc ncggtgagaa
      961 tggcaaaagc ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc
     1021 atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct ga
//
