LOCUS Exported 6731 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Express GATA3 C2 in mammalian cells. ACCESSION . VERSION . KEYWORDS pLPCX2-HA-GATA3 C1(295-443) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6731) AUTHORS Sun J, He H, Pillai S, Xiong Y, Challa S, Xu L, Chellappan S, Yang S TITLE GATA3 transcription factor abrogates Smad4 transcription factor-mediated fascin overexpression, invadopodium formation, and breast cancer cell invasion. JOURNAL J Biol Chem. 2013 Dec 27;288(52):36971-82. doi: 10.1074/jbc.M113.506535. Epub 2013 Nov 14. PUBMED 24235142 REFERENCE 2 (bases 1 to 6731) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6731 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..304 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 305..508 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 459..479 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" CDS 561..587 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" LTR 1132..1725 /note="long terminal repeat from Moloney murine leukemia virus" primer_bind complement(1828..1850) /label=pGEX 3' /note="pGEX vectors, reverse primer" protein_bind complement(1978..2011) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." protein_bind complement(2071..2104) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." primer_bind 2124..2143 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind complement(2224..2241) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(2395..2983) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2475..2494) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(3154..4014) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 3777..3796 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(4015..4119) /gene="bla" /label=AmpR promoter primer_bind 4187..4205 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" LTR 4807..4982 /label=5' LTR (truncated) /note="truncated long terminal repeat from Moloney murine sarcoma virus" misc_feature 5045..5244 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 5445..5861 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 5791..5813 /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" primer_bind 5829..5845 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" CDS 5958..6557 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(5958..5977) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 6454..6474 /label=Puro-F /note="Puromycin resistance gene, forward primer" ORIGIN 1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 541 ccggactcag atctatggac tacccttatg atgtgccgga ttatgccggg cagaaccggc 601 cccttatcaa gcccaagcga aggctgtcgg cagcaaggag agcagggaca tcctgcgcga 661 actgtcagac caccaccacc accctctgga ggaggaacgc taatggggac ccggtctgca 721 atgcctgtgg gctgtactac aagcttcata atattaacag acccctgact atgaagaaag 781 aaggcatcca gacccgaaac cggaagatgt ctagcaaatc gaaaaagtgc aaaaaggtgc 841 atgacgcgct ggaggacttc cccaagagca gctccttcaa cccggccgct ctctccagac 901 acatgtcatc cctgagccac atctctccct tcagccactc cagccacatg ctgaccacac 961 cgacgcccat gcatccgccc tccggcctct ccttcggacc tcaccaccct tccagcatgg 1021 tcaccgccat gggtagaatt cgttaggcca ttaaggcctg tcgacaagcg gccgcctcgg 1081 ccaaacatcg ataaaataaa agattttatt tagtctccag aaaaaggggg gaatgaaaga 1141 ccccacctgt aggtttggca agctagctta agtaacgcca ttttgcaagg catggaaaaa 1201 tacataactg agaatagaga agttcagatc aaggtcagga acagatggaa cagctgaata 1261 tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga 1321 tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc 1381 agggccaaga acagatggtc cccagatgcg gtccagccct cagcagtttc tagagaacca 1441 tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac 1501 caatcagttc gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag 1561 cccacaaccc ctcactcggg gcgccagtcc tccgattgac tgagtcgccc gggtacccgt 1621 gtatccaata aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg 1681 gtctcctctg agtgattgac tacccgtcag cgggggtctt tcatttgggg gctcgtccgg 1741 gatcgggaga cccctgccca gggaccaccg acccaccacc gggaggtaag ctggctgcct 1801 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac 1861 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt 1921 tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtactgata 1981 acttcgtata atgtatgcta tacgaagtta ttaggtctga agaggagttt acgtccagcc 2041 aagctagggc cgcgatccgg aacccttaat ataacttcgt ataatgtatg ctatacgaag 2101 ttatcagtac tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat 2161 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc 2221 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 2281 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 2341 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 2401 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 2461 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 2521 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 2581 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 2641 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 2701 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 2761 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 2821 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 2881 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 2941 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 3001 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 3061 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 3121 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 3181 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 3241 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 3301 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 3361 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 3421 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt 3481 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 3541 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 3601 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 3661 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 3721 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa 3781 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 3841 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 3901 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 3961 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 4021 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 4081 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 4141 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac 4201 gaggcccttt cgtcttcaag aattgctagc aattgctagc aattgctagc aattcatacc 4261 agatcaccga aaactgtcct ccaaatgtgt ccccctcaca ctcccaaatt cgcgggcttc 4321 tgcctcttag accactctac cctattcccc acactcaccg gagccaaagc cgcggccctt 4381 ccgtttcttt gcttttgaaa gaccccaccc gtaggtggca agctagctta agtaacgcca 4441 ctttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc aaggtcagga 4501 acaaagaaac agctgaatac caaacaggat atctgtggta agcggttcct gccccggctc 4561 agggccaaga acagatgaga cagctgagtg atgggccaaa caggatatct gtggtaagca 4621 gttcctgccc cggctcgggg ccaagaacag atggtcccca gatgcggtcc agccctcagc 4681 agtttctagt gaatcatcag atgtttccag ggtgccccaa ggacctgaaa atgaccctgt 4741 accttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttccgctctc 4801 cgagctcaat aaaagagccc acaacccctc actcggcgcg ccagtcttcc gatagactgc 4861 gtcgcccggg tacccgtatt cccaataaag cctcttgctg tttgcatccg aatcgtggtc 4921 tcgctgttcc ttgggagggt ctcctctgag tgattgacta cccacgacgg gggtctttca 4981 tttgggggct cgtccgggat ttggagaccc ctgcccaggg accaccgacc caccaccggg 5041 aggtaagctg gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgtttgatg 5101 ttatgcgcct gcgtctgtac tagttagcta actagctctg tatctggcgg acccgtggtg 5161 gaactgacga gttctgaaca cccggccgca accctgggag acgtcccagg gactttgggg 5221 gccgtttttg tggcccgacc tgaggaaggg agtcgatgtg gaatccgacc ccgtcaggat 5281 atgtggttct ggtaggagac gagaacctaa aacagttccc gcctccgtct gaatttttgc 5341 tttcggtttg gaaccgaagc cgcgcgtctt gtctgctgca gcgctgcagc atcgttctgt 5401 gttgtctctg tctgactgtg tttctgtatt tgtctgaaaa ttagggccag actgttacca 5461 ctcccttaag tttgacctta ggtcactgga aagatgtcga gcggatcgct cacaaccagt 5521 cggtagatgt caagaagaga cgttgggtta ccttctgctc tgcagaatgg ccaaccttta 5581 acgtcggatg gccgcgagac ggcaccttta accgagacct catcacccag gttaagatca 5641 aggtcttttc acctggcccg catggacacc cagaccaggt cccctacatc gtgacctggg 5701 aagccttggc ttttgacccc cctccctggg tcaagccctt tgtacaccct aagcctccgc 5761 ctcctcttcc tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg accccgcctc 5821 gatcctccct ttatccagcc ctcactcctt ctctaggcgc cggaatgatc ccccgggctg 5881 caggtcggcc gccacgaccg gtgccgccac catcccctga cccacgcccc tgacccctca 5941 caaggagacg accttccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg 6001 acgtcccccg ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc 6061 acaccgtcga cccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca 6121 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg 6181 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca 6241 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc 6301 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc 6361 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg 6421 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg 6481 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc 6541 gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa aggagcgcac 6601 gaccccatgg ctccgaccga agccacccgg ggcggccccg ccgaccccgc acccgccccc 6661 gaggcccacc gactctagta atagtaatca attacggggt cattagttca tagcccatat 6721 atggagttcc g //