LOCUS Exported 9223 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS PBCAG-EGFR SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9223) AUTHORS Chen F, LoTurco J TITLE A method for stable transgenesis of radial glia lineage in rat neocortex by piggyBac mediated transposition. JOURNAL J Neurosci Methods. 2012 Jun 15;207(2):172-80. Epub 2012 Apr 11. PUBMED 22521325 REFERENCE 2 (bases 1 to 9223) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9223 /organism="synthetic DNA construct" /mol_type="other DNA" repeat_region 12..74 /label=piggyBac right (3') inverted repeat /note="piggyBac transposon-specific inverted terminal repeat sequence (ITR)" protein_bind complement(371..404) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." enhancer 500..803 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 805..1080 /label=chicken beta-actin promoter intron 1081..2089 /label=chimeric intron /note="chimera between introns from chicken beta-actin and rabbit beta-globin" primer_bind 2013..2035 /label=pCAGGS-5 /note="Chimeric intron in CAG promoter, forward primer" primer_bind 2097..2116 /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" CDS 2160..5792 /codon_start=1 /product="precursor of human epidermal growth factor receptor" /label=EGFR /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG CPTNGPKISSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSP KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA" primer_bind complement(5878..5897) /label=Bglob-pA-R /note="Rabbit beta-globin polyA region, reverse primer" polyA_signal 5943..5998 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" primer_bind complement(5997..6016) /label=rbglobpA-R /note="Rabbit beta-globin polyA, reverse primer. Also called rb-glob-pA-term-R" repeat_region 6669..6703 /label=piggyBac left (5') inverted repeat /note="piggyBac transposon-specific inverted terminal repeat sequence (ITR)" primer_bind complement(6726..6742) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6726..6742) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(6739..6761) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 6750..6766 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(6774..6804) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6819..6840 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 6899..7094 /label=SV40 promoter /note="SV40 early promoter" rep_origin 6945..7080 /label=SV40 ori /note="SV40 origin of replication" primer_bind 7007..7026 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" polyA_signal 7100..7234 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(7137..7156) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 7191..7210 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(7302..7319) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(7473..8061) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(7553..7572) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(8232..9092) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 8855..8874 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(9093..9197) /gene="bla" /label=AmpR promoter ORIGIN 1 ggtcgactta accctagaaa gataatcata ttgtgacgta cgttaaagat aatcatgcgt 61 aaaattgacg catgtgtttt atcgatctgt atatcgaggt ttatttatta atttgaatag 121 atattaagtt ttattatatt tacacttaca tactaataat aaattcaaca aacaatttat 181 ttatgtttat ttatttatta aaaaaaaaca aaaactcaaa atttcttcta taaagtaaca 241 aaacttttaa acattctctc ttttacaaaa ataaacttat tttgtacttt aaaaacagtc 301 atgttgtatt ataaaataag taattagctt aacttataca taatagaaac aaattatact 361 tattaatcgc ataacttcgt ataatgtatg ctatacgaag ttatgcgatt aagggatctg 421 tagggcgcag tagtccaact agttattaat agtaatcaat tacggggtca ttagttcata 481 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 541 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 601 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac 661 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg 721 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg 781 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca 841 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag 901 cgatgggggc gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg 961 ggcggggcga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt 1021 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg 1081 ggagtcgctg cgttgccttc gccccgtgcc ccgctccgcg ccgcctcgcg ccgcccgccc 1141 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg 1201 ggctgtaatt agcgcttggt ttaatgacgg ctcgtttctt ttctgtggct gcgtgaaagc 1261 cttaaagggc tccgggaggg ccctttgtgc gggggggagc ggctcggggg gtgcgtgcgt 1321 gtgtgtgtgc gtggggagcg ccgcgtgcgg cccgcgctgc ccggcggctg tgagcgctgc 1381 gggcgcggcg cggggctttg tgcgctccgc gtgtgcgcga ggggagcgcg gccgggggcg 1441 gtgccccgcg gtgcgggggg gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt 1501 gggggggtga gcagggggtg tgggcgcggc ggtcgggctg taaccccccc ctgcaccccc 1561 ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtgcg gggcgtggcg 1621 cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc 1681 cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg ccccggagcg ccggcggctg 1741 tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg 1801 acttcctttg tcccaaatct ggcggagccg aaatctggga ggcgccgccg caccccctct 1861 agcgggcgcg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc 1921 gtgcgtcgcc gcgccgccgt ccccttctcc atctccagcc tcggggctgc cgcaggggga 1981 cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg 2041 ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa 2101 cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttctgcagtc gacggtacca 2161 tgcgaccctc cgggacggcc ggggcagcgc tcctggcgct gctggctgcg ctctgcccgg 2221 cgagtcgggc tctggaggaa aagaaagttt gccaaggcac gagtaacaag ctcacgcagt 2281 tgggcacttt tgaagatcat tttctcagcc tccagaggat gttcaataac tgtgaggtgg 2341 tccttgggaa tttggaaatt acctatgtgc agaggaatta tgatctttcc ttcttaaaga 2401 ccatccagga ggtggctggt tatgtcctca ttgccctcaa cacagtggag cgaattcctt 2461 tggaaaacct gcagatcatc agaggaaata tgtactacga aaattcctat gccttagcag 2521 tcttatctaa ctatgatgca aataaaaccg gactgaagga gctgcccatg agaaatttac 2581 aggaaatcct gcatggcgcc gtgcggttca gcaacaaccc tgccctgtgc aacgtggaga 2641 gcatccagtg gcgggacata gtcagcagtg actttctcag caacatgtcg atggacttcc 2701 agaaccacct gggcagctgc caaaagtgtg atccaagctg tcccaatggg agctgctggg 2761 gtgcaggaga ggagaactgc cagaaactga ccaaaatcat ctgtgcccag cagtgctccg 2821 ggcgctgccg tggcaagtcc cccagtgact gctgccacaa ccagtgtgct gcaggctgca 2881 caggcccccg ggagagcgac tgcctggtct gccgcaaatt ccgagacgaa gccacgtgca 2941 aggacacctg ccccccactc atgctctaca accccaccac gtaccagatg gatgtgaacc 3001 ccgagggcaa atacagcttt ggtgccacct gcgtgaagaa gtgtccccgt aattatgtgg 3061 tgacagatca cggctcgtgc gtccgagcct gtggggccga cagctatgag atggaggaag 3121 acggcgtccg caagtgtaag aagtgcgaag ggccttgccg caaagtgtgt aacggaatag 3181 gtattggtga atttaaagac tcactctcca taaatgctac gaatattaaa cacttcaaaa 3241 actgcacctc catcagtggc gatctccaca tcctgccggt ggcatttagg ggtgactcct 3301 tcacacatac tcctcctctg gatccacagg aactggatat tctgaaaacc gtaaaggaaa 3361 tcacagggtt tttgctgatt caggcttggc ctgaaaacag gacggacctc catgcctttg 3421 agaacctaga aatcatacgc ggcaggacca agcaacatgg tcagttttct cttgcagtcg 3481 tcagcctgaa cataacatcc ttgggattac gctccctcaa ggagataagt gatggagatg 3541 tgataatttc aggaaacaaa aatttgtgct atgcaaatac aataaactgg aaaaaactgt 3601 ttgggacctc cggtcagaaa accaaaatta taagcaacag aggtgaaaac agctgcaagg 3661 ccacaggcca ggtctgccat gccttgtgct cccccgaggg ctgctggggc ccggagccca 3721 gggactgcgt ctcttgccgg aatgtcagcc gaggcaggga atgcgtggac aagtgcaacc 3781 ttctggaggg tgagccaagg gagtttgtgg agaactctga gtgcatacag tgccacccag 3841 agtgcctgcc tcaggccatg aacatcacct gcacaggacg gggaccagac aactgtatcc 3901 agtgtgccca ctacattgac ggcccccact gcgtcaagac ctgcccggca ggagtcatgg 3961 gagaaaacaa caccctggtc tggaagtacg cagacgccgg ccatgtgtgc cacctgtgcc 4021 atccaaactg cacctacgga tgcactgggc caggtcttga aggctgtcca acgaatgggc 4081 ctaagatctc gtccatcgcc actgggatgg tgggggccct cctcttgctg ctggtggtgg 4141 ccctggggat cggcctcttc atgcgaaggc gccacatcgt tcggaagcgc acgctgcgga 4201 ggctgctgca ggagagggag cttgtggagc ctcttacacc cagtggagaa gctcccaacc 4261 aagctctctt gaggatcttg aaggaaactg aattcaaaaa gatcaaagtg ctgggctccg 4321 gtgcgttcgg cacggtgtat aagggactct ggatcccaga aggtgagaaa gttaaaattc 4381 ccgtcgctat caaggaatta agagaagcaa catctccgaa ggccaacaag gaaatcctcg 4441 atgaagccta cgtgatggcc agcgtggaca acccccacgt gtgccgcctg ctgggcatct 4501 gcctcacctc caccgtgcaa ctcatcacgc agctcatgcc cttcggctgc ctcctggact 4561 atgtccggga acacaaagac aatattggct cccagtacct gctcaactgg tgtgtgcaga 4621 tcgcaaaggg catgaactac ttggaggacc gtcgcttggt gcaccgcgac ctggcagcca 4681 ggaacgtact ggtgaaaaca ccgcagcatg tcaagatcac agattttggg ctggccaaac 4741 tgctgggtgc ggaagagaaa gaataccatg cagaaggagg caaagtgcct atcaagtgga 4801 tggcattgga atcaatttta cacagaatct atacccacca gagtgatgtc tggagctacg 4861 gggtgaccgt ttgggagttg atgacctttg gatccaagcc atatgacgga atccctgcca 4921 gcgagatctc ctccatcctg gagaaaggag aacgcctccc tcagccaccc atatgtacca 4981 tcgatgtcta catgatcatg gtcaagtgct ggatgataga cgcagatagt cgcccaaagt 5041 tccgtgagtt gatcatcgaa ttctccaaaa tggcccgaga cccccagcgc taccttgtca 5101 ttcaggggga tgaaagaatg catttgccaa gtcctacaga ctccaacttc taccgtgccc 5161 tgatggatga agaagacatg gacgacgtgg tggatgccga cgagtacctc atcccacagc 5221 agggcttctt cagcagcccc tccacgtcac ggactcccct cctgagctct ctgagtgcaa 5281 ccagcaacaa ttccaccgtg gcttgcattg atagaaatgg gctgcaaagc tgtcccatca 5341 aggaagacag cttcttgcag cgatacagct cagaccccac aggcgccttg actgaggaca 5401 gcatagacga caccttcctc ccagtgcctg aatacataaa ccagtccgtt cccaaaaggc 5461 ccgctggctc tgtgcagaat cctgtctatc acaatcagcc tctgaacccc gcgcccagca 5521 gagacccaca ctaccaggac ccccacagca ctgcagtggg caaccccgag tatctcaaca 5581 ctgtccagcc cacctgtgtc aacagcacat tcgacagccc tgcccactgg gcccagaaag 5641 gcagccacca aattagcctg gacaaccctg actaccagca ggacttcttt cccaaggaag 5701 ccaagccaaa tggcatcttt aagggctcca cagctgaaaa tgcagaatac ctaagggtcg 5761 cgccacaaag cagtgaattt attggagcat gagcggccgc actcctcagg tgcaggctgc 5821 ctatcagaag gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct 5881 ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg 5941 ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact 6001 cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag 6061 tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca 6121 tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg 6181 aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt 6241 ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc 6301 tgtccctctt ctcttatgga gatccctcga cctgcagagc tccagcctac acaatcgctc 6361 aagacgtgta atgcttttat tatatattag tcacgatatc tataacaaga aaatatatat 6421 ataataagtt atcacgtaag tagaacatga aataacaata taattatcgt atgagttaaa 6481 tcttaaaagt cacgtaaaag ataatcatgc gtcattttga ctcacgcggt cgttatagtt 6541 caaaatcagt gacacttacc gcattgacaa gcacgcctca cgggagctcc aagcggcgac 6601 tgagatgtcc taaatgcaca gcgacggatt cgcgctattt agaaagagag agcaatattt 6661 caagaatgca tgcgtcaatt ttacgcagac tatctttcta gggttaaaag cttggcgtaa 6721 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata 6781 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta 6841 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gcggatccgc 6901 atctcaataa gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc 6961 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 7021 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 7081 taggcttttg caaaaagcta acttgtttat tgcagcttat aatggttaca aataaagcaa 7141 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 7201 caaactcatc aatgtatctt atcatgtctg gatccgctgc attaatgaat cggccaacgc 7261 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg 7321 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 7381 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 7441 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 7501 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 7561 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 7621 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 7681 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 7741 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 7801 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 7861 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta 7921 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 7981 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 8041 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 8101 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 8161 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 8221 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 8281 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 8341 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 8401 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 8461 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 8521 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 8581 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 8641 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 8701 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 8761 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 8821 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact 8881 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 8941 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 9001 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 9061 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 9121 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 9181 caaatagggg ttccgcgcac atttccccga aaagtgccac ctg //