LOCUS Exported 9272 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expresses humanized LbCpf1 variant that recognizes TYCV PAMs. ACCESSION . VERSION . KEYWORDS pcDNA3.1-hLbCpf1(TYCV) (pY230) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9272) AUTHORS Gao L, Cox DBT, Yan WX, Manteiga JC, Schneider MW, Yamano T, Nishimasu H, Nureki O, Crosetto N, Zhang F TITLE Engineered Cpf1 variants with altered PAM specificities. JOURNAL Nat Biotechnol. 2017 Jun 5. doi: 10.1038/nbt.3900. PUBMED 28581492 REFERENCE 2 (bases 1 to 9272) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9272 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 11..32 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(151..171) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 156..485 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 336..471 /label=SV40 ori /note="SV40 origin of replication" primer_bind 398..417 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 552..1346 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(606..625) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 1216..1235 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 1520..1641 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1557..1576) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 1611..1630 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(1690..1706) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1690..1706) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(1703..1725) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 1714..1730 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1738..1768) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 1783..1804 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(1921..1938) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(2092..2680) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2172..2191) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(2851..3711) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 3474..3493 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(3712..3816) /gene="bla" /label=AmpR promoter primer_bind complement(3891..3910) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 4082..4461 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 4462..4665 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 4616..4636 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 4710..4729 /label=T7 /note="T7 promoter, forward primer" promoter 4710..4728 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 4764..4773 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 4776..4796 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 4821..8501 /codon_start=1 /product="CRISPR-associated protein from Lachnospiraceae bacterium ND2006 (Zetsche et al., 2015)" /label=LbCpf1 /translation="SKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAED YKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKEI AKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFSE EAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEGE FFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVLS DRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKNG PAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQL QEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKDL LDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYSK DKFKLYFQNPQFMRGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNGN YEKINYKLLPGPNKMLPRVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKLI DFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLVE EGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKKE ELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKIN TEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTDY HSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSGF KNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQN GFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALDY KNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQQ GDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRNY EAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTSV KH" CDS 8502..8549 /codon_start=1 /product="bipartite nuclear localization signal from nucleoplasmin" /label=nucleoplasmin NLS /translation="KRPAATKKAGQAKKKK" CDS 8556..8636 /codon_start=1 /product="three tandem HA epitope tags" /label=3xHA /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA" primer_bind complement(8710..8727) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 8716..8940 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 8986..142 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(9073..9092) /label=F1ori-R /note="F1 origin, reverse primer" ORIGIN 1 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat 61 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt 121 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt 181 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca 241 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt 301 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt 361 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg 421 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt 481 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag 541 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg 601 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt 661 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc 721 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt 781 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag 841 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg 901 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag 961 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg 1021 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc 1081 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca 1141 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc 1201 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg 1261 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct 1321 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc 1381 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg 1441 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct 1501 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa 1561 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 1621 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc 1681 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 1741 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 1801 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 1861 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 1921 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 1981 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 2041 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 2101 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 2161 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 2221 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 2281 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 2341 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 2401 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 2461 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 2521 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 2581 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 2641 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 2701 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 2761 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 2821 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 2881 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 2941 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 3001 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 3061 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 3121 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 3181 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 3241 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 3301 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 3361 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 3421 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 3481 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 3541 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 3601 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 3661 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 3721 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 3781 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 3841 tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc 3901 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag 3961 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag 4021 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt 4081 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc 4141 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc 4201 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg 4261 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat 4321 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc 4381 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta 4441 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag 4501 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt 4561 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa 4621 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctctg gctaactaga 4681 gaacccactg cttactggct tatcgaaatt aatacgactc actataggga gacccaagct 4741 ggctagcgtt taaacttaag cttgccacca tggccccaaa gaagaagcgg aaggtcggta 4801 tccacggagt cccagcagcc agcaagctgg agaagtttac aaactgctac tccctgtcta 4861 agaccctgag gttcaaggcc atccctgtgg gcaagaccca ggagaacatc gacaataagc 4921 ggctgctggt ggaggacgag aagagagccg aggattataa gggcgtgaag aagctgctgg 4981 atcgctacta tctgtctttt atcaacgacg tgctgcacag catcaagctg aagaatctga 5041 acaattacat cagcctgttc cggaagaaaa ccagaaccga gaaggagaat aaggagctgg 5101 agaacctgga gatcaatctg cggaaggaga tcgccaaggc cttcaagggc aacgagggct 5161 acaagtccct gtttaagaag gatatcatcg agacaatcct gccagagttc ctggacgata 5221 aggacgagat cgccctggtg aacagcttca atggctttac cacagccttc accggcttct 5281 ttgataacag agagaatatg ttttccgagg aggccaagag cacatccatc gccttcaggt 5341 gtatcaacga gaatctgacc cgctacatct ctaatatgga catcttcgag aaggtggacg 5401 ccatctttga taagcacgag gtgcaggaga tcaaggagaa gatcctgaac agcgactatg 5461 atgtggagga tttctttgag ggcgagttct ttaactttgt gctgacacag gagggcatcg 5521 acgtgtataa cgccatcatc ggcggcttcg tgaccgagag cggcgagaag atcaagggcc 5581 tgaacgagta catcaacctg tataatcaga aaaccaagca gaagctgcct aagtttaagc 5641 cactgtataa gcaggtgctg agcgatcggg agtctctgag cttctacggc gagggctata 5701 catccgatga ggaggtgctg gaggtgttta gaaacaccct gaacaagaac agcgagatct 5761 tcagctccat caagaagctg gagaagctgt tcaagaattt tgacgagtac tctagcgccg 5821 gcatctttgt gaagaacggc cccgccatca gcacaatctc caaggatatc ttcggcgagt 5881 ggaacgtgat ccgggacaag tggaatgccg agtatgacga tatccacctg aagaagaagg 5941 ccgtggtgac cgagaagtac gaggacgatc ggagaaagtc cttcaagaag atcggctcct 6001 tttctctgga gcagctgcag gagtacgccg acgccgatct gtctgtggtg gagaagctga 6061 aggagatcat catccagaag gtggatgaga tctacaaggt gtatggctcc tctgagaagc 6121 tgttcgacgc cgattttgtg ctggagaaga gcctgaagaa gaacgacgcc gtggtggcca 6181 tcatgaagga cctgctggat tctgtgaaga gcttcgagaa ttacatcaag gccttctttg 6241 gcgagggcaa ggagacaaac agggacgagt ccttctatgg cgattttgtg ctggcctacg 6301 acatcctgct gaaggtggac cacatctacg atgccatccg caattatgtg acccagaagc 6361 cctactctaa ggataagttc aagctgtatt ttcagaaccc tcagttcatg cgcggctggg 6421 acaaggataa ggagacagac tatcgggcca ccatcctgag atacggctcc aagtactatc 6481 tggccatcat ggataagaag tacgccaagt gcctgcagaa gatcgacaag gacgatgtga 6541 acggcaatta cgagaagatc aactataagc tgctgcccgg ccctaataag atgctgccaa 6601 gggtgttctt ttctaagaag tggatggcct actataaccc cagcgaggac atccagaaga 6661 tctacaagaa tggcacattc aagaagggcg atatgtttaa cctgaatgac tgtcacaagc 6721 tgatcgactt ctttaaggat agcatctccc ggtatccaaa gtggtccaat gcctacgatt 6781 tcaacttttc tgagacagag aagtataagg acatcgccgg cttttacaga gaggtggagg 6841 agcagggcta taaggtgagc ttcgagtctg ccagcaagaa ggaggtggat aagctggtgg 6901 aggagggcaa gctgtatatg ttccagatct ataacaagga cttttccgat aagtctcacg 6961 gcacacccaa tctgcacacc atgtacttca agctgctgtt tgacgagaac aatcacggac 7021 agatcaggct gagcggagga gcagagctgt tcatgaggcg cgcctccctg aagaaggagg 7081 agctggtggt gcacccagcc aactccccta tcgccaacaa gaatccagat aatcccaaga 7141 aaaccacaac cctgtcctac gacgtgtata aggataagag gttttctgag gaccagtacg 7201 agctgcacat cccaatcgcc atcaataagt gccccaagaa catcttcaag atcaatacag 7261 aggtgcgcgt gctgctgaag cacgacgata acccctatgt gatcggcatc gataggggcg 7321 agcgcaatct gctgtatatc gtggtggtgg acggcaaggg caacatcgtg gagcagtatt 7381 ccctgaacga gatcatcaac aacttcaacg gcatcaggat caagacagat taccactctc 7441 tgctggacaa gaaggagaag gagaggttcg aggcccgcca gaactggacc tccatcgaga 7501 atatcaagga gctgaaggcc ggctatatct ctcaggtggt gcacaagatc tgcgagctgg 7561 tggagaagta cgatgccgtg atcgccctgg aggacctgaa ctctggcttt aagaatagcc 7621 gcgtgaaggt ggagaagcag gtgtatcaga agttcgagaa gatgctgatc gataagctga 7681 actacatggt ggacaagaag tctaatcctt gtgcaacagg cggcgccctg aagggctatc 7741 agatcaccaa taagttcgag agctttaagt ccatgtctac ccagaacggc ttcatctttt 7801 acatccctgc ctggctgaca tccaagatcg atccatctac cggctttgtg aacctgctga 7861 aaaccaagta taccagcatc gccgattcca agaagttcat cagctccttt gacaggatca 7921 tgtacgtgcc cgaggaggat ctgttcgagt ttgccctgga ctataagaac ttctctcgca 7981 cagacgccga ttacatcaag aagtggaagc tgtactccta cggcaaccgg atcagaatct 8041 tccggaatcc taagaagaac aacgtgttcg actgggagga ggtgtgcctg accagcgcct 8101 ataaggagct gttcaacaag tacggcatca attatcagca gggcgatatc agagccctgc 8161 tgtgcgagca gtccgacaag gccttctact ctagctttat ggccctgatg agcctgatgc 8221 tgcagatgcg gaacagcatc acaggccgca ccgacgtgga ttttctgatc agccctgtga 8281 agaactccga cggcatcttc tacgatagcc ggaactatga ggcccaggag aatgccatcc 8341 tgccaaagaa cgccgacgcc aatggcgcct ataacatcgc cagaaaggtg ctgtgggcca 8401 tcggccagtt caagaaggcc gaggacgaga agctggataa ggtgaagatc gccatctcta 8461 acaaggagtg gctggagtac gcccagacca gcgtgaagca caaaaggccg gcggccacga 8521 aaaaggccgg ccaggcaaaa aagaaaaagg gatcctaccc atacgatgtt ccagattacg 8581 cttatcccta cgacgtgcct gattatgcat acccatatga tgtccccgac tatgcctaag 8641 aattctgcag atatccagca cagtggcggc cgctcgagtc tagagggccc gtttaaaccc 8701 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg 8761 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa 8821 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca 8881 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg 8941 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg 9001 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg 9061 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc 9121 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc 9181 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga 9241 cggtttttcg ccctttgacg ttggagtcca cg //