LOCUS       Exported                 871 bp ds-DNA     linear   SYN 19-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pcDNA3 HA Arf1 DN T31N
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Furman C, Short SM, Subramanian RR, Zetter BR, Roberts TM
  TITLE     DEF-1/ASAP1 is a GTPase-activating protein (GAP) for ARF1 that 
            enhances cell motility through a GAP-dependent mechanism.
  JOURNAL   J Biol Chem. 2002 Mar 8. 277(10):7962-9.
  PUBMED    11773070
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             569..595
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(670..688)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(671..688)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(708..725)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    714..825
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ggccgccagt gtgctggaat tccatatggg gaacatcttc gccaacctct tcaagggcct
       61 ttttggcaaa aaagaaatgc gcatcctcat ggtgggcctg gatgctgcag ggaagaacac
      121 gatcctctac aagcttaagc tgggtgagat cgtgaccacc attcccacca taggcttcaa
      181 cgtggaaacc gtggagtaca agaacatcag cttcactgtg tgggacgtgg gtggccagga
      241 caagatccgg cccctgtggc gccactactt ccagaacaca caaggcctga tcttcgtggt
      301 ggacagcaat gacagagagc gtgtgaacga ggcccgtgag gagctcatga ggatgctggc
      361 cgaggacgag ctccgggatg ctgtcctcct ggtgttcgcc aacaagcagg acctccccaa
      421 cgccatgaat gcggccgaga tcacagacaa gctggggctg cactcactac gccacaggaa
      481 ctggtacatt caggccacct gcgccaccag cggcgacggg ctctatgaag gactggactg
      541 gctgtccaat cagctccgga accagaagta cccatacgat gttccagatt acgcttaata
      601 gtctagacag ctgagatctg atatccatca cactggcggc cgctcgagca tgcatctaga
      661 gggccctatt ctatagtgtc acctaaatgc tagagctcgc tgatcagcct cgactgtgcc
      721 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
      781 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
      841 gtgtcattct attctggggg gtggggtggg g
//
