LOCUS Exported 927 bp ds-DNA linear SYN 29-MAY-2021 DEFINITION slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue to green light, inactivation max with 605 nm (accelarates closure to ms). Codon optimized for mammalian expression.. ACCESSION . VERSION . KEYWORDS p-mCherry-N1-iChloC_CA SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 927) AUTHORS Wietek J, Rodriguez-Rozada S, Tutas J, Tenedini F, Grimm C, Oertner TG, Soba P, Hegemann P, Wiegert JS TITLE Anion-conducting channelrhodopsins with tuned spectra and modified kinetics engineered for optogenetic manipulation of behavior. JOURNAL Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.1038/s41598-017-14330-y. PUBMED 29097684 REFERENCE 2 (bases 1 to 927) AUTHORS . TITLE Direct Submission JOURNAL Exported May 29, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..927 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 1..927 /codon_start=1 /product="H134R mutant of an N-terminal fragment of channelrhodopsin-2 from the alga Chlamydomonas reinhardtii (Nagel et al., 2005)" /label=ChR2(H134R) /note="blue light-activated cation channel" /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEQIYVCAIRMVKVILEFFFSFKN PSMLYLATGHRVQWLRYAEWLLTAPVILIHLSNLTGLSNDYSRRTMGLLVSNIGCIVWG ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI RKTTKLNIGGTEIEVETLVEDEAEAGAV" ORIGIN 1 atggactatg gcggcgcttt gtctgccgtc ggacgcgaac ttttgttcgt tactaatcct 61 gtggtggtga acgggtccgt cctggtccct gaggatcaat gttactgtgc cggatggatt 121 gaatctcgcg gcacgaacgg cgctcagacc gcgtcaaatg tcctgcagtg gcttgcagca 181 ggattcagca ttttgctgct gatgttctat gcctaccaaa cctggaaatc tacatgcggc 241 tgggagcaga tctatgtgtg cgccattaga atggttaagg tgattctcga gttctttttt 301 agctttaaga atccctctat gctctacctt gccacaggac accgggtgca gtggctgcgc 361 tatgcagagt ggctgctcac tgctcctgtc atccttatcc acctgagcaa cctcaccggc 421 ctgagcaacg actacagcag gagaaccatg ggactccttg tctcaaacat cgggtgtatc 481 gtgtgggggg ctaccagcgc catggcaacc ggctatgtta aagtcatctt cttttgtctt 541 ggattgtgct atggcgcgaa cacatttttt cacgccgcca aagcatatat cgagggttat 601 catactgtgc caaagggtcg gtgccgccag gtcgtgaccg gcatggcatg gctgtttttc 661 gtgagctggg gtatgttccc aattctcttc attttggggc ccgaaggttt tggcgtcctg 721 agcgtctatg gctccaccgt aggtcacacg attattgatc tgatgagtaa aaattgttgg 781 gggttgttgg gacactacct gcgcgtcctg atccacgagc acatattgat tcacggagat 841 atccgcaaaa ccaccaaact gaacatcggc ggaacggaga tcgaggtcga gactctcgtc 901 gaagacgaag ccgaggccgg agccgtg //