LOCUS       Exported                 713 bp ds-DNA     linear   SYN 18-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pDEST-periHisMBP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Nallamsetty S, Austin BP, Penrose KJ, Waugh DS
  TITLE     Gateway vectors for the production of combinatorially-tagged 
            His6-MBP fusion proteins in the cytoplasm and periplasm of 
            Escherichia coli.
  JOURNAL   Protein Sci. 2005 Dec . 14(12):2964-71.
  PUBMED    16322578
REFERENCE   2  (bases 1 to 713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             113..130
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gagcacttcc caacaaggac catagattat gaaaataaaa acaggtgcac gcatcctcgc
       61 attatccgca ttaacgacga tgatgttttc cgcctcggct ctcgccaaaa tccatcacca
      121 tcaccatcac gaagaaggta aactggtaat ctggattaac ggcgataaag gctataacgg
      181 tctcgctgaa gtcggtaaga aattcgagaa agataccgga attaaagtca ccgttgagca
      241 tccggataaa ctggaagaga aattcccaca ggttgcggca actggcgatg gccctgacat
      301 tatcttctgg gcacacgacc gctttggtgg ctacgctcaa tctggcctgt tggctgaaat
      361 caccccggac aaagcgttcc aggacaagct gtatccgttt acctgggatg ccgtacgtta
      421 caacggcaag ctgattgctt acccgatcgc tgttgaagcg ttatcgctga tttataacaa
      481 agatctgctg ccgaacccgc caaaaacctg ggaagagatc ccggcgctgg ataaagaact
      541 gaaagcgaaa ggtaagagcg cgctgatgtt caacctgcaa gaaccgtact tcacctggcc
      601 gctgattgct gctgacgggg gttatgcgtt caagtatgaa aacggcaagt acgacattaa
      661 agacgtgggc gtggataacg ctggcgcgaa agcgggtctg accttcctgg ttg
//
