LOCUS Exported 9843 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expresses YFP-NaVII-III from the Synapsin promoter, for neuron-specific and activity-independent live label of the Axon Initial Segment (AIS). ACCESSION . VERSION . KEYWORDS pl-Synapsin-YFP-NaVII-III SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9843) AUTHORS Dumitrescu AS, Evans MD, Grubb MS TITLE Evaluating Tools for Live Imaging of Structural Plasticity at the Axon Initial Segment. JOURNAL Front Cell Neurosci. 2016 Nov 23;10:268. eCollection 2016. PUBMED 27932952 REFERENCE 2 (bases 1 to 9843) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9843 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(47..66) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 238..617 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 619..817 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 772..792 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" LTR 835..1015 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" misc_feature 1062..1187 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 1680..1913 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 2098..2142 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 2440..2557 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 2632..3079 /label=hSyn promoter /note="human synapsin I promoter; confers neuron-specific expression (Kugler et al., 2003)" regulatory 3093..3102 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3099..3815 /codon_start=1 /product="enhanced YFP" /label=EYFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(3144..3165) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(3405..3424) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 3752..3773 /label=EGFP-C /note="EGFP, forward primer" misc_feature 4535..5123 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(4588..4608) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(5006..5017) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind complement(5126..5142) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(5127..5143) /label=pBluescriptKS /note="For pBluescript vector" LTR 5648..5828 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(5854..5871) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 5860..6084 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 6130..6558 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6217..6236) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 6427..6448 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(6567..6587) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 6572..6901 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 6752..6887 /label=SV40 ori /note="SV40 origin of replication" primer_bind 6814..6833 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 6949..6996 /label=EM7 promoter /note="synthetic bacterial promoter " CDS 7015..7389 /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" polyA_signal 7519..7640 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(7556..7575) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 7610..7629 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(7689..7705) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7689..7705) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(7702..7724) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 7713..7729 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(7737..7767) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7782..7803 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(7920..7937) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8091..8679) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8171..8190) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(8850..9710) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 9473..9492 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(9711..9815) /gene="bla" /label=AmpR promoter ORIGIN 1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2581 agatccagtt tggttaatta agtgtctaga ctgcagaggg ccctgcgtat gagtgcaagt 2641 gggttttagg accaggatga ggcggggtgg gggtgcctac ctgacgaccg accccgaccc 2701 actggacaag cacccaaccc ccattcccca aattgcgcat cccctatcag agagggggag 2761 gggaaacagg atgcggcgag gcgcgtgcgc actgccagct tcagcaccgc ggacagtgcc 2821 ttcgcccccg cctggcggcg cgcgccaccg ccgcctcagc actgaaggcg cgctgacgtc 2881 actcgccggt cccccgcaaa ctccccttcc cggccacctt ggtcgcgtcc gcgccgccgc 2941 cggcccagcc ggaccgcacc acgcgaggcg cgagataggg gggcacgggc gcgaccatct 3001 gcgctgcggc gccggcgact cagcgctgcc tcagtctgcg gtgggcagcg gaggagtcgt 3061 gtcgtgcctg agagcgcagt cgagaaaccg gtgccaccat ggtgagcaag ggcgaggagc 3121 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt 3181 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca 3241 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttcggctacg 3301 gcctgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg 3361 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca 3421 agacccgcgc cgaggtgaag ttcgagggtg acaccctggt gaaccgcatc gagctgaagg 3481 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca 3541 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga 3601 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc 3661 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac cagtccgccc 3721 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg 3781 ccgggatcac tctcggcatg gacgagctgt acaagagttc tttcagctca gacaacctgg 3841 ctgccacaga tgacgataac gaaatgaaca acctccagat agccgtggga aggatgcaga 3901 agggaattga ttttgttaaa aggaagatac gtgaattcat tcagaaagcc tttgtcagaa 3961 agcagaaagc tttagatgaa atcaaaccgc tggaagatct gaataacaag aaagacagtt 4021 gtatctccaa ccacacgacc atagaaatag gcaaggacct caattacctc aaagatggaa 4081 acgggacgac cagtggcata ggcagcagtg tggagaagta tgtggtagat gagagtgatt 4141 acatgtcgtt cataaacaac cccagcctca ccgtgactgt gcccatcgcc cttggagagt 4201 ctgactttga aaacttaaac acggaagaat tcagtagtga gtcagatatg gaagaaagca 4261 aggagaaatt gaatgcaact agctcatctg agggcagtac ggttgatata ggcgctccgg 4321 cagagggaga gcagccagag gccgaaccgg aagaatcgct tgaaccggaa gcgtgtttca 4381 cagaagactg tgtgagaaag ttcaagtgtt gtcagataag catagaagag ggcaaaggca 4441 agctctggtg gaacttgagg aaaacgtgct acaagataag ctagctgtac aagtaagaat 4501 tcgaaagggc gaattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg 4561 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc 4621 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta 4681 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt 4741 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca 4801 gctcctttcc ggaactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc 4861 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt 4921 gtcggggaaa tcatcgtcct ttccttggct gctcgcctat gttgccacct ggattctgcg 4981 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg 5041 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat 5101 ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgagacctag aaaaacatgg 5161 agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca 5221 agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta 5281 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat 5341 tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt 5401 ccctgattgg cagaactaca caccagggcc agggatcaga tatccactga cctttggatg 5461 gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa 5521 cacccgcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtatt 5581 agagtggagg tttgacagcc gcctagcatt tcatcacatg gcccgagagc tgcatccgga 5641 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg 5701 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg 5761 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat 5821 ctctagcagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc 5881 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg 5941 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc 6001 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg 6061 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg 6121 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca 6181 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct 6241 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt 6301 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac 6361 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct 6421 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt 6481 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac 6541 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc 6601 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg 6661 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc 6721 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc 6781 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc 6841 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa 6901 aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa 6961 tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc 7021 aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc 7081 tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc 7141 cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc 7201 ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg 7261 tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg 7321 gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag 7381 caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc 7441 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg 7501 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat 7561 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 7621 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg 7681 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac 7741 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca 7801 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat 7861 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc 7921 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 7981 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 8041 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 8101 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 8161 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 8221 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 8281 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 8341 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 8401 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 8461 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 8521 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 8581 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 8641 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 8701 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 8761 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa 8821 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc 8881 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact 8941 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc 9001 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt 9061 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 9121 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg 9181 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt 9241 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc 9301 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt 9361 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc 9421 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc 9481 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa 9541 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac 9601 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa 9661 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt 9721 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa 9781 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct 9841 gac //