LOCUS       Exported                 846 bp ds-DNA     linear   SYN 11-JUN-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    832 pcDNA3 GFP PTEN delta274-342
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 846)
  TITLE     PTEN
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 11, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     27..46
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        27..45
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             65..778
                     /codon_start=1
                     /product="Aequoria victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(95..123)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     718..738
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 cccactgctt actggcttat cgaaattaat acgactcact atagggagac ccaagcttgc
       61 caccatgagt aaaggagaag aactcttcac tggagttgtc ccaattcttg ttgaattaga
      121 tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata
      181 cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac
      241 acttgtcact actttcactt atggtgttca gtgcttttca agatacccgg atcatatgaa
      301 acggcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt
      361 tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct
      421 tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttgggca
      481 caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa
      541 tggaatcaaa gctaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc
      601 agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca
      661 ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt
      721 ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaaca
      781 gcagcttcag ggatccatga cagccatcat caaagagatc gttagcagaa acaaaaggag
      841 atatca
//
