LOCUS Exported 1519 bp ds-DNA linear SYN 25-MAY-2021 DEFINITION In vivo visualization of the ER (can be used for colocalization studies and the OSER assay). ACCESSION . VERSION . KEYWORDS CytERM-mKOkappa SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1519) TITLE Gadella lab plasmids REFERENCE 2 (bases 1 to 1519) AUTHORS . TITLE Direct Submission JOURNAL Exported May 25, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1519 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 97..400 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 401..604 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 555..575 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 826..835 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 853..1500 /codon_start=1 /product="monomeric Kusabira-Orange 2 fluorescent protein" /label=mKO2 /translation="SVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVTM AEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSASV SAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYLK LEGGGNHKCQFKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS" ORIGIN 1 cctgattctg tggataaccg tattaccgcc atgcattagt tattaatagt aatcaattac 61 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg 121 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc 181 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac 241 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa 301 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac 361 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta 421 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga 481 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa 541 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag 601 agctggttta gtgaaccgtc agatccgcta gcgctaccgg actcagatct ggtacccctt 661 gcagcttcca gttgtcagga agagaaggct cccatggatc ctgtggtggt gctggggctc 721 tgtctctcct gtttgcttct cctttcactc tggaaacaga gctatggggg agggaagctt 781 cgaattctgc agtcgacggt accgcgggcc cgggatccac cggtcgccac catggtgagc 841 aagggcgagg agagtgtgat taaaccagag atgaagatga ggtactacat ggacggctcc 901 gtcaatgggc atgagttcac aattgaaggt gaaggcacag gcagacctta cgagggacat 961 caagagatga cactacgcgt cacaatggcc gagggcgggc caatgccttt cgcgtttgac 1021 ttagtgtcac acgtgttctg ttacggccac agagtattta ctaaatatcc agaagagata 1081 ccagactatt tcaaacaagc atttcctgaa ggcctgtcat gggaaaggtc gttggagttc 1141 gaagatggtg ggtccgcttc agtcagtgcg catataagcc ttagaggaaa caccttctac 1201 cacaaatcca aatttactgg ggttaacttt cctgccgatg gtcctatcat gcaaaaccaa 1261 agtgttgatt gggagccatc aaccgagaaa attactgcca gcgacggagt tctgaagggt 1321 gatgttacga tgtacctaaa acttgaagga ggcggcaatc acaaatgcca attcaagact 1381 acttacaagg cggcaaaaga gattcttgaa atgccaggag accattacat cggccatcgc 1441 ctcgtcagga aaaccgaagg caacattact gagcaggtag aagatgcagt agctcattcc 1501 actggcgacg agctgtaca //