LOCUS Exported 5486 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pSG5-HA-ERR3 S45A SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5486) AUTHORS Heckler MM, Thakor H, Schafer CC, Riggins RB TITLE ERK/MAPK regulates ERRgamma expression, transcriptional activity, and receptor-mediated Tamoxifen resistance in ER+ breast cancer. JOURNAL FEBS J. 2014 Mar 29. doi: 10.1111/febs.12797. PUBMED 24684682 REFERENCE 2 (bases 1 to 5486) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5486 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 1..27 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" CDS 231..242 /codon_start=1 /product="WELQut protease recognition and cleavage site" /label=WELQut site /translation="WELQ" polyA_signal 1431..1552 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1468..1487) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 1522..1541 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(1586..1603) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1757..2345) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1837..1856) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(2516..3376) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 3139..3158 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(3377..3481) /gene="bla" /label=AmpR promoter primer_bind 3549..3567 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(3605..3627) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 3727..3746 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" rep_origin complement(3813..4268) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3950..3971) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 4162..4181 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4394..4416 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 4408..4425 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 4409..4425 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4462..4482) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 4570..4780 /label=SV40 promoter /note="SV40 early promoter" promoter 4592..4788 /label=SV40 promoter /note="SV40 early promoter" rep_origin 4639..4774 /label=SV40 ori /note="SV40 origin of replication" primer_bind 4701..4720 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" intron 4819..5391 /label=-beta-globin intron /note="intron from rabbit beta-globin gene" primer_bind 5399..5418 /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" primer_bind 5446..5465 /label=T7 /note="T7 promoter, forward primer" promoter 5446..5464 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 5475..5484 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" ORIGIN 1 tacccatacg atgttcctga ctatgcggaa ttctcgagtg attcggtaga actttgcctg 61 cctgaatctt tttccctgca ctacgaagaa gagcttctct gcagaatgtc aaacaaagat 121 cgacacattg attccagctg ttcgtccttc atcaagacgg aaccctccgc cccagcctcc 181 ctgacggaca gcgtcaacca ccacagccct ggtgggtctt ccgacgccag tgggagttac 241 agttcaacca tgaatggcca tcagaacgga ctggactcgc cacctctcta cccctctgct 301 ccgatcctgg gaggcagcgg gcctgtccgg aaactgtatg atgactgctc cagcaccatc 361 gtagaggatc cccagaccaa gtgtgaatat atgctcaact ccatgcccaa gagactgtgc 421 ttagtgtgtg gcgacatcgc ctctgggtac cactatgggg ttgcatcatg tgaagcctgc 481 aaggcattct tcaagaggac gattcaaggt aacatagagt acagctgccc agccacgaat 541 gaatgtgaga tcacaaagcg cagacgcaaa tcctgccagg cctgccgctt catgaagtgt 601 ctcaaagtgg gcatgctgaa agaaggggtc cgtcttgaca gagtgcgtgg aggtcggcag 661 aagtacaagc gcagaataga tgctgagaac agcccatacc tgaaccctca gctggtgcag 721 ccagccaaaa agccatataa caagattgtc tcgcatttgt tggtggctga accagagaag 781 atctatgcca tgcctgaccc tactgtcccc gacagtgaca tcaaagccct caccacactc 841 tgtgacttgg ctgaccgaga gttggtggtt atcattggat gggcaaaaca tattccaggc 901 ttctccacac tgtcccttgc agaccagatg agcctcctcc agagtgcatg gatggagatt 961 ctgatcctcg gcgttgtgta ccgatcgctt tcgtttgagg atgaacttgt ctatgcagac 1021 gattatataa tggatgaaga ccagtctaaa ttagcaggcc ttcttgacct aaataatgct 1081 atcctgcagc tggtgaagaa gtacaagagc atgaagctag agaaggaaga attcgtcacc 1141 ctcaaagcaa tagctcttgc taattcagat tccatgcata tagaagatgt ggaagctgtg 1201 cagaaacttc aggatgtgtt acatgaggcc ctgcaggatt acgaggctgg ccagcacatg 1261 gaagaccctc gccgtgcagg caagatgctg atgacgctgc cgctgctgag gcagacctcc 1321 accaaggcag tccagcactt ctacaacatc aaactcgaag gcaaagtgcc catgcacaaa 1381 ctttttttgg aaatgctgga ggccaaggtc tgaggatctt attaaagcag aacttgttta 1441 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 1501 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct 1561 ggtcgactct agactcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 1621 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 1681 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 1741 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 1801 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 1861 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 1921 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 1981 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 2041 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 2101 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 2161 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc 2221 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 2281 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 2341 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 2401 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 2461 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 2521 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 2581 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 2641 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 2701 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 2761 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 2821 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 2881 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 2941 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 3001 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 3061 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 3121 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 3181 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 3241 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 3301 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 3361 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 3421 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 3481 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa 3541 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg 3601 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg 3661 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta 3721 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc 3781 acagatgcgt aaggagaaaa taccgcatca ggaaattgta agcgttaata ttttgttaaa 3841 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa 3901 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa 3961 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca 4021 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg 4081 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc 4141 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc 4201 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca 4261 gggcgcgtcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc 4321 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta 4381 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtcgacttct 4441 gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct 4501 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaagg aaagtcccca 4561 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc 4621 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc 4681 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta 4741 ttccagaagt agtgaagagg cttttttgga ggcctaggct tttgcaaaaa gctccggatc 4801 gatcctgaga acttcagggt gagtttgggg acccttgatt gttctttctt tttcgctatt 4861 gtaaaattca tgttatatgg agggggcaaa gttttcaggg tgttgtttag aatgggaaga 4921 tgtcccttgt atcaccatgg accctcatga taattttgtt tctttcactt tctactctgt 4981 tgacaaccat tgtctcctct tattttcttt tcattttctg taactttttc gttaaacttt 5041 agcttgcatt tgtaacgaat ttttaaattc acttttgttt atttgtcaga ttgtaagtac 5101 tttctctaat cacttttttt tcaaggcaat cagggtatat tatattgtac ttcagcacag 5161 ttttagagaa caattgttat aattaaatga taaggtagaa tatttctgca tataaattct 5221 ggctggcgtg gaaatattct tattggtaga aacaactaca tcctggtcat catcctgcct 5281 ttctctttat ggttacaatg atatacactg tttgagatga ggataaaata ctctgagtcc 5341 aaaccgggcc cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc 5401 aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aattgtaata cgactcacta 5461 tagggcgaat taccgccgcc atgggc //