LOCUS Exported 8829 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION human VEGFR1 with V to A mutation at position 767. ACCESSION . VERSION . KEYWORDS hFlt1 V767A SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8829) AUTHORS Raikwar NS, Liu KZ, Thomas CP TITLE Ectodomain cleavage of FLT1 regulates receptor activation and function and is not required for its downstream intracellular cleavage. JOURNAL Exp Cell Res. 2016 May 15;344(1):103-11. doi: 10.1016/j.yexcr.2016.03.020. Epub 2016 Mar 23. PUBMED 27017929 REFERENCE 2 (bases 1 to 8829) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8829 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(35..54) /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind 645..664 /label=Neo-R /note="Neomycin resistance gene, reverse primer" rep_origin 767..901 /label=SV40 ori /note="SV40 origin of replication" primer_bind complement(821..840) /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind 974..994 /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter complement(1019..1123) /gene="bla" /label=AmpR promoter rep_origin 1150..1605 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1237..1256) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 1447..1468 /label=F1ori-F /note="F1 origin, forward primer" primer_bind 1731..1753 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 1745..1762 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 1746..1762 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" enhancer 1923..2302 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 2303..2506 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 2457..2477 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 2532..2551 /label=T7 /note="T7 promoter, forward primer" promoter 2532..2550 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 2699..2725 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" CDS 6668..6697 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 6716..6739 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" primer_bind complement(6759..6775) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6759..6775) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" polyA_signal 6786..7408 /label=hGH poly(A) signal /note="human growth hormone polyadenylation signal" primer_bind complement(6985..7004) /label=hGH-PA-R /note="Human growth hormone terminator, reverse primer" rep_origin complement(7557..8145) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(7637..7656) /label=pBR322ori-F /note="pBR322 origin, forward primer" polyA_signal 8474..8521 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" primer_bind 8546..8565 /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" CDS complement(8753..718) /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" ORIGIN 1 agcggtcagc ccattcgccg ccaagctctt cagcaatatc acgggtagcc aacgctatgt 61 cctgatagcg atccgccaca cccagccggc cacagtcgat gaatccagaa aagcggccat 121 tttccaccat gatattcggc aagcaggcat cgccatgggt cacgacgaga tcctcgccgt 181 cgggcatgct cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt 241 cgtccagatc atcctgatcg acaagaccgg cttccatccg agtacgtgct cgctcgatgc 301 gatgtttcgc ttggtggtcg aatgggcagg tagccggatc aagcgtatgc agccgccgca 361 ttgcatcagc catgatggat actttctcgg caggagcaag gtgagatgac aggagatcct 421 gccccggcac ttcgcccaat agcagccagt cccttcccgc ttcagtgaca acgtcgagca 481 cagctgcgca aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc tcgtcttgca 541 gttcattcag ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg 601 acagccggaa cacggcggca tcagagcagc cgattgtctg ttgtgcccag tcatagccga 661 atagcctctc cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc 721 gaaacgatcc tcatcctgtc tcttgatcga tctttgcaaa agcctaggcc tccaaaaaag 781 cctcctcact acttctggaa tagctcagag gccgaggcgg cctcggcctc tgcataaata 841 aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt taggggcggg 901 actatggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct 961 ggggactttc cacaccctaa ctgacacaca ttccacagct ggttctttcc gcctcaggac 1021 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 1081 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 1141 tgccacctga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca 1201 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct 1261 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt 1321 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac 1381 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct 1441 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt 1501 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac 1561 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttccatt cgccattcag 1621 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc 1681 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg 1741 acgttgtaaa acgacggcca gtgccaagct gatctataca ttgaatcaat attggcaatt 1801 agccatatta gtcattggtt atatagcata aatcaatatt ggctattggc cattgcatac 1861 gttgtatcta tatcataata tgtacattta tattggctca tgtccaatat gaccgccatg 1921 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag 1981 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc 2041 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg 2101 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca 2161 tcaagtgtat catatgccaa gtccgccccc tattgacgtc aatgacggta aatggcccgc 2221 ctggcattat gcccagtaca tgaccttacg ggactttcct acttggcagt acatctacgt 2281 attagtcatc gctattacca tggtgatgcg gttttggcag tacaccaatg ggcgtggata 2341 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt 2401 ttggcaccaa aatcaacggg actttccaaa atgtcgtaat aaccccgccc cgttgacgca 2461 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg 2521 tcagaatttt gtaatacgac tcactatagg gcggccggga attcgtcgac tggatccggt 2581 accgaggaga tctgccgccg cgatcgccat ggtcagctac tgggacaccg gggtcctgct 2641 gtgcgcgctg ctcagctgtc tgcttctcac aggatctagt tcaggttcaa aattaaaata 2701 cccatatgac gtcccagact acgctgatcc tgaactgagt ttaaaaggca cccagcacat 2761 catgcaagca ggccagacac tgcatctcca atgcaggggg gaagcagccc ataaatggtc 2821 tttgcctgaa atggtgagta aggaaagcga aaggctgagc ataactaaat ctgcctgtgg 2881 aagaaatggc aaacaattct gcagtacttt aaccttgaac acagctcaag caaaccacac 2941 tggcttctac agctgcaaat atctagctgt acctacttca aagaagaagg aaacagaatc 3001 tgcaatctat atatttatta gtgatacagg tagacctttc gtagagatgt acagtgaaat 3061 ccccgaaatt atacacatga ctgaaggaag ggagctcgtc attccctgcc gggttacgtc 3121 acctaacatc actgttactt taaaaaagtt tccacttgac actttgatcc ctgatggaaa 3181 acgcataatc tgggacagta gaaagggctt catcatatca aatgcaacgt acaaagaaat 3241 agggcttctg acctgtgaag caacagtcaa tgggcatttg tataagacaa actatctcac 3301 acatcgacaa accaatacaa tcatagatgt ccaaataagc acaccacgcc cagtcaaatt 3361 acttagaggc catactcttg tcctcaattg tactgctacc actcccttga acacgagagt 3421 tcaaatgacc tggagttacc ctgatgaaaa aaataagaga gcttccgtaa ggcgacgaat 3481 tgaccaaagc aattcccatg ccaacatatt ctacagtgtt cttactattg acaaaatgca 3541 gaacaaagac aaaggacttt atacttgtcg tgtaaggagt ggaccatcat tcaaatctgt 3601 taacacctca gtgcatatat atgataaagc attcatcact gtgaaacatc gaaaacagca 3661 ggtgcttgaa accgtagctg gcaagcggtc ttaccggctc tctatgaaag tgaaggcatt 3721 tccctcgccg gaagttgtat ggttaaaaga tgggttacct gcgactgaga aatctgctcg 3781 ctatttgact cgtggctact cgttaattat caaggacgta actgaagagg atgcagggaa 3841 ttatacaatc ttgctgagca taaaacagtc aaatgtgttt aaaaacctca ctgccactct 3901 aattgtcaat gtgaaacccc agatttacga aaaggccgtg tcatcgtttc cagacccggc 3961 tctctaccca ctgggcagca gacaaatcct gacttgtacc gcatatggta tccctcaacc 4021 tacaatcaag tggttctggc acccctgtaa ccataatcat tccgaagcaa ggtgtgactt 4081 ttgttccaat aatgaagagt cctttatcct ggatgctgac agcaacatgg gaaacagaat 4141 tgagagcatc actcagcgca tggcaataat agaaggaaag aataagatgg ctagcacctt 4201 ggttgtggct gactctagaa tttctggaat ctacatttgc atagcttcca ataaagttgg 4261 gactgtggga agaaacataa gcttttatat cacagatgtg ccaaatgggt ttcatgttaa 4321 cttggaaaaa atgccgacgg aaggagagga cctgaaactg tcttgcacag ttaacaagtt 4381 cttatacaga gacgttactt ggattttact gcggacagtt aataacagaa caatgcacta 4441 cagtattagc aagcaaaaaa tggccatcac taaggagcac tccatcactc ttaatcttac 4501 catcatgaat gtttccctgc aagattcagg cacctatgcc tgcagagcca ggaatgtata 4561 cacaggggaa gaaatcctcc agaagaaaga aattacaatc agagatcagg aagcaccata 4621 cctcctgcga aacctcagtg atcacacagt ggccatcagc agttccacca ctttagactg 4681 tcatgctaat ggtgtccccg agcctcagat cacttggttt aaaaacaacc acaaaataca 4741 acaagagcct ggaattattt taggaccagg aagcagcacg ctgtttattg aaagagtcac 4801 agaagaggat gaaggtgtct atcactgcaa agccaccaac cagaagggct ctgtggaaag 4861 ttcagcatac ctcactgttc aaggaacctc ggacaagtct aatctggagc tgatcactct 4921 aacatgcacc tgtgcggctg cgactctctt ctggctccta ttaaccctct ttatccgaaa 4981 aatgaaaagg tcttcttctg aaataaagac tgactaccta tcaattataa tggacccaga 5041 tgaagttcct ttggatgagc agtgtgagcg gctcccttat gatgccagca agtgggagtt 5101 tgcccgggag agacttaaac tgggcaaatc acttggaaga ggggcttttg gaaaagtggt 5161 tcaagcatca gcatttggca ttaagaaatc acctacgtgc cggactgtgg ctgtgaaaat 5221 gctgaaagag ggggccacgg ccagcgagta caaagctctg atgactgagc taaaaatctt 5281 gacccacatt ggccaccatc tgaacgtggt taacctgctg ggagcctgca ccaagcaagg 5341 agggcctctg atggtgattg ttgaatactg caaatatgga aatctctcca actacctcaa 5401 gagcaaacgt gacttatttt ttctcaacaa ggatgcagca ctacacatgg agcctaagaa 5461 agaaaaaatg gagccaggcc tggaacaagg caagaaacca agactagata gcgtcaccag 5521 cagcgaaagc tttgcgagct ccggctttca ggaagataaa agtctgagtg atgttgagga 5581 agaggaggat tctgacggtt tctacaagga gcccatcact atggaagatc tgatttctta 5641 cagttttcaa gtggccagag gcatggagtt cctgtcttcc agaaagtgca ttcatcggga 5701 cctggcagcg agaaacattc ttttatctga gaacaacgtg gtgaagattt gtgattttgg 5761 ccttgcccgg gatatttata agaaccccga ttatgtgaga aaaggagata ctcgacttcc 5821 tctgaaatgg atggctcccg aatctatctt tgacaaaatc tacagcacca agagcgacgt 5881 gtggtcttac ggagtattgc tgtgggaaat cttctcctta ggtgggtctc catacccagg 5941 agtacaaatg gatgaggact tttgcagtcg cctgagggaa ggcatgagga tgagagctcc 6001 tgagtactct actcctgaaa tctatcagat catgctggac tgctggcaca gagacccaaa 6061 agaaaggcca agatttgcag aacttgtgga aaaactaggt gatttgcttc aagcaaatgt 6121 acaacaggat ggtaaagact acatcccaat caatgccata ctgacaggaa atagtgggtt 6181 tacatactca actcctgcct tctctgagga cttcttcaag gaaagtattt cagctccgaa 6241 gtttaattca ggaagctctg atgatgtcag atatgtaaat gctttcaagt tcatgagcct 6301 ggaaagaatc aaaacctttg aagaactttt accgaatgcc acctccatgt ttgatgacta 6361 ccagggcgac agcagcactc tgttggcctc tcccatgctg aagcgcttca cctggactga 6421 cagcaaaccc aaggcctcgc tcaagattga cttgagagta accagtaaaa gtaaggagtc 6481 ggggctgtct gatgtcagca ggcccagttt ctgccattcc agctgtgggc acgtcagcga 6541 aggcaagcgc aggttcacct acgaccacgc tgagctggaa aggaaaatcg cgtgctgctc 6601 cccgccccca gactacaact cggtggtcct gtactccacc ccacccatca cgcgtacgcg 6661 gccgctcgag cagaaactca tctcagaaga ggatctggca gcaaatgata tcctggatta 6721 caaggatgac gacgataagg tttaaacggc cggccgcggt catagctgtt tcctgaacag 6781 atcccgggtg gcatccctgt gacccctccc cagtgcctct cctggccctg gaagttgcca 6841 ctccagtgcc caccagcctt gtcctaataa aattaagttg catcattttg tctgactagg 6901 tgtccttcta taatattatg gggtggaggg gggtggtatg gagcaagggg caagttggga 6961 agacaacctg tagggcctgc ggggtctatt gggaaccaag ctggagtgca gtggcacaat 7021 cttggctcac tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct cagcctcccg 7081 agttgttggg attccaggca tgcatgacca ggctcagcta atttttgttt ttttggtaga 7141 gacggggttt caccatattg gccaggctgg tctccaactc ctaatctcag gtgatctacc 7201 caccttggcc tcccaaattg ctgggattac aggcgtgaac cactgctccc ttccctgtcc 7261 ttctgatttt aaaataacta taccagcagg aggacgtcca gacacagcat aggctacctg 7321 gccatgccca accggtggga catttgagtt gcttgcttgg cactgtcctc tcatgcgttg 7381 ggtccactca gtagatgcct gttgaattgg gtacgcggcc agcggcgagc ggtatcagct 7441 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 7501 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc 7561 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 7621 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 7681 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 7741 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag 7801 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat 7861 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac 7921 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac 7981 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc 8041 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt 8101 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc 8161 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg 8221 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca 8281 atctaaagta tatatgagta acctgaggct atggcagggc ctgccgcccc gacgttggct 8341 gcgagccctg ggccttcacc cgaacttggg gggtggggtg gggaaaagga agaaacgcgg 8401 gcgtattggc cccaatgggg tctcggtggg gtatcgacag agtgccagcc ctgggaccga 8461 accccgcgtt tatgaacaaa cgacccaaca ccgtgcgttt tattctgtct ttttattgcc 8521 gtcatagcgc gggttccttc cggtattgtc tccttccgtg tttcagttag cctcccccta 8581 gggtgggcga agaactccag catgagatcc ccgcgctgga ggatcatcca gccggcgtcc 8641 cggaaaacga ttccgaagcc caacctttca tagaaggcgg cggtggaatc gaaatctcgt 8701 gatggcaggt tgggcgtcgc ttggtcggtc atttcgaacc ccagagtccc gctcagaaga 8761 actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa 8821 gcacgagga //