LOCUS Exported 9542 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Twinstrep-SUMO-huLwCas13a for recombinant protein bacterial expression. Insert is human codon optimized but expresses well in bacteria.. ACCESSION . VERSION . KEYWORDS pC013 - Twinstrep-SUMO-huLwCas13a SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9542) AUTHORS Gootenberg JS, Abudayyeh OO, Lee JW, Essletzbichler P, Dy AJ, Joung J, Verdine V, Donghia N, Daringer NM, Freije CA, Myhrvold C, Bhattacharyya RP, Livny J, Regev A, Koonin EV, Hung DT, Sabeti PC, Collins JJ, Zhang F TITLE Nucleic acid detection with CRISPR-Cas13a/C2c2. JOURNAL Science. 2017 Apr 13. pii: eaam9321. doi: 10.1126/science.aam9321. PUBMED 28408723 REFERENCE 2 (bases 1 to 9542) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9542 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(170..192) /label=pGEX 3' /note="pGEX vectors, reverse primer" misc_feature 278..417 /label=bom /note="basis of mobility region from pBR322" primer_bind 333..352 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind complement(432..449) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(603..1191) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(683..702) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1362..2222) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 1985..2004 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(2223..2327) /gene="bla" /label=AmpR promoter primer_bind 2395..2413 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 2440..2468 /label=tet promoter /note="E. coli promoter for tetracycline efflux protein gene" terminator 2643..2690 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2686..2704 /label=T7 Term /note="T7 terminator, reverse primer" CDS complement(2754..6209) /codon_start=1 /product="Leptotrichia wadei CRISPR-Cas effector that acts as an RNA-guided RNAse (Abudayyeh et al., 2017)" /label=LwCas13a /note="previously called C2c2" /translation="MKVTKVDGISHKKYIEEGKLVKSTSEENRTSERLSELLSIRLDIY IKNPDNASEEENRIRRENLKKFFSNKVLHLKDSVLYLKNRKEKNAVQDKNYSEEDISEY DLKNKNSFSVLKKILLNEDVNSEELEIFRKDVEAKLNKINSLKYSFEENKANYQKINEN NVEKVGGKSKRNIIYDYYRESAKRNDYINNVQEAFDKLYKKEDIEKLFFLIENSKKHEK YKIREYYHKIIGRKNDKENFAKIIYEEIQNVNNIKELIEKIPDMSELKKSQVFYKYYLD KEELNDKNIKYAFCHFVEIEMSQLLKNYVYKRLSNISNDKIKRIFEYQNLKKLIENKLL NKLDTYVRNCGKYNYYLQVGEIATSDFIARNRQNEAFLRNIIGVSSVAYFSLRNILETE NENDITGRMRGKTVKNNKGEEKYVSGEVDKIYNENKQNEVKENLKMFYSYDFNMDNKNE IEDFFANIDEAISSIRHGIVHFNLELEGKDIFAFKNIAPSEISKKMFQNEINEKKLKLK IFKQLNSANVFNYYEKDVIIKYLKNTKFNFVNKNIPFVPSFTKLYNKIEDLRNTLKFFW SVPKDKEEKDAQIYLLKNIYYGEFLNKFVKNSKVFFKITNEVIKINKQRNQKTGHYKYQ KFENIEKTVPVEYLAIIQSREMINNQDKEEKNTYIDFIQQIFLKGFIDYLNKNNLKYIE SNNNNDNNDIFSKIKIKKDNKEKYDKILKNYEKHNRNKEIPHEINEFVREIKLGKILKY TENLNMFYLILKLLNHKELTNLKGSLEKYQSANKEETFSDELELINLLNLDNNRVTEDF ELEANEIGKFLDFNENKIKDRKELKKFDTNKIYFDGENIIKHRAFYNIKKYGMLNLLEK IADKAKYKISLKELKEYSNKKNEIEKNYTMQQNLHRKYARPKKDEKFNDEDYKEYEKAI GNIQKYTHLKNKVEFNELNLLQGLLLKILHRLVGYTSIWERDLRFRLKGEFPENHYIEE IFNFDNSKNVKYKSGQIVEKYINFYKELYKDNVEKRSIYSDKKVKKLKQEKKDLYIRNY IAHFNYIPHAEISLLEVLENLRKLLSYDRKLKNAIMKSIVDILKEYGFVATFKIGADKK IEIQTLESEKIVHLKNLKKKKLMTDRNSEELCELVKVMFEYKALE" CDS complement(6216..6506) /codon_start=1 /gene="S. cerevisiae SMT3 (truncated)" /product="cleavable ubiquitin-like protein tag" /label=SUMO /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIG" CDS complement(6507..6590) /codon_start=1 /product="two Strep-Tag(R)II moieties connected by a linker for enhanced binding to Strep-Tactin(R), an engineered form of streptavidin (Schmidt et al., 2013)" /label=Twin-Strep-tag /translation="WSHPQFEKGGGSGGGSGGSAWSHPQFEK" CDS complement(6603..6620) /codon_start=1 /product="thrombin recognition and cleavage site" /label=thrombin site /translation="LVPRGS" CDS complement(6630..6647) /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" RBS 6666..6688 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" protein_bind 6703..6727 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind complement(6727..6746) /label=T7 /note="T7 promoter, forward primer" promoter complement(6728..6746) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(6830..6849) /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" promoter 7055..7132 /gene="lacI" /label=lacI promoter CDS 7133..8215 /codon_start=1 /gene="lacI" /product="lac repressor" /label=lacI /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR ALADSLMQLARQVSRLESGQ" primer_bind complement(7152..7171) /label=LacI-R /note="LacI, reverse primer" CDS 9527..176 /codon_start=1 /gene="rop" /product="Rop protein, which maintains plasmids at low copy number" /label=rop /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA DELYRSCLARFGDDGENL" ORIGIN 1 aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt ctggagaaac 61 tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac gaccacgctg 121 atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca 181 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc 241 gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta 301 gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt 361 gcaccattgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc 421 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 481 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 541 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 601 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 661 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 721 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 781 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 841 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 901 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 961 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 1021 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt 1081 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 1141 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 1201 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 1261 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 1321 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 1381 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 1441 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 1501 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 1561 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 1621 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca 1681 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga 1741 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct 1801 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg 1861 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca 1921 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca 1981 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct 2041 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact 2101 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa 2161 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc 2221 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga 2281 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga 2341 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg 2401 cgtatcacga ggccctttcg tcttcaagaa ttctcatgtt tgacagctta tcatcgataa 2461 gctttaatgc ggtagtttat cacagttaaa ttgctaacgc agtcaggcac cgtgtatgaa 2521 atctaacaat gcgctcatcg tcatcctcgg caccgtcacc ctggatgctg taggcatagg 2581 cttggttatg ccggtactgc cgggcctctt gcgggatatc cggatatagt tcctcctttc 2641 agcaaaaaac ccctcaagac ccgtttagag gccccaaggg gttatgctag ttattgctca 2701 gcggtggcag cagccaactc agcttccttt cgggcctcga gtgcggccgc ttattccagg 2761 gccttgtact cgaacatgac tttcacgagt tcgcacagtt cctcgctgtt ccggtcggtc 2821 atcagtttct ttttcttcag attcttcagg tgcacgatct tctctgattc cagggtctgg 2881 atttcgatct tcttgtcagc gccgatcttg aaggtggcca cgaagccgta ttctttcaga 2941 atgtccacga tggacttcat gatggcgttc ttcagcttcc ggtcgtagga cagcagcttc 3001 cgcaggtttt ccagcacttc cagcaggcta atctcggcgt gggggatgta gttgaagtgg 3061 gcaatgtagt tccggatgta caggtccttt ttttcctgct tcagtttctt cactttcttg 3121 tcggagtaga tgctccgctt ttccacattg tccttgtaca gttctttgta gaagttgata 3181 tacttttcca cgatctggcc gcttttgtac ttcacattct tggagttgtc gaaattgaaa 3241 atttcctcga tgtagtggtt ctcgggaaac tcgcccttca gccggaatct caggtcccgc 3301 tcccagatgc tggtgtagcc cacgagccgg tgcaggatct tcagcagcag gccctgcagc 3361 aggttcagct cattgaattc caccttgttc ttcaggtggg tgtacttctg gatgttgccg 3421 atggccttct catactcttt gtagtcctcg tcgttgaact tttcgtcctt cttgggtctg 3481 gcgtacttcc ggtgcaggtt ctgctgcatg gtgtagttct tttcaatctc attcttcttg 3541 ttgctgtact ctttcagttc tttcaggctg atcttatact tggccttatc ggcgatcttt 3601 tccagcagat tcagcatgcc gtatttcttg atattgtaga aggcccggtg cttgatgatg 3661 ttctcgccgt cgaaatagat cttgttggtg tcgaactttt tcagctcttt ccggtccttg 3721 attttgtttt cgttgaagtc caggaacttg ccgatctcgt tggcttccag ctcgaagtcc 3781 tcggtcactc tgttgttgtc caggttcagc aggttgatca gttccagctc gtcgctgaag 3841 gtttcttctt tgttggcgga ctggtacttt tccaggctgc ccttcaggtt ggtcagctct 3901 ttgtggttca gcagcttcag gatcaggtaa aacatgttca gattctcggt gtacttcaga 3961 atcttcccca gcttgatctc gcgcacgaac tcattgatct cgtgagggat ttctttgttc 4021 cgattgtgct tctcatagtt cttcaggatc ttgtcgtact tctctttgtt atcctttttg 4081 atcttgatct tggagaagat gtcgttgttg tcattgttgt tgttgctctc gatatacttc 4141 agattgttct tgttcaggta gtcgatgaag cccttcagga aaatctgctg aataaagtcg 4201 atgtaggtat tcttttcctc tttgtcctgg ttgttgatca tctctctgct ctggatgatg 4261 gccaggtatt ccacgggcac ggttttctcg atgttctcga acttctgata cttgtagtgg 4321 ccggttttct ggttccgctg cttgttaatc ttgatcactt cattggtgat cttaaagaac 4381 accttggagt ttttcacgaa cttgttcagg aactcgccgt agtagatatt cttcagcagg 4441 tagatctggg cgtccttctc ttctttgtcc ttgggcacgc tccaaaaaaa cttcagggta 4501 ttccgcaggt cctcaatctt gttgtacagc ttggtgaagc tgggcacgaa ggggatgttt 4561 ttgttcacga agttgaactt ggtattcttc aggtacttga tgatcacatc cttctcgtag 4621 tagttgaaca cgttggcgct gttcagctgc ttgaagattt tcagcttcag cttcttttcg 4681 ttgatttcgt tctgaaacat cttcttggag atctcgctgg gggcgatatt cttgaaggcg 4741 aagatgtcct tgccttccag ttccaggttg aagtgcacga tgccgtgtct gatgctgctg 4801 atggcctcgt cgatgttggc gaagaagtcc tcgatctcgt tcttgttgtc catgttgaag 4861 tcgtagctgt agaacatctt cagattttct ttcacttcgt tctgcttgtt ctcattgtag 4921 atcttgtcca cctcgccgga cacgtatttc tcttcgccct tgttgttctt cacggtcttg 4981 ccccgcatcc ggccggtgat atcgttctcg ttctcggttt ccaggatgtt cctcaggctg 5041 aagtaggcca cgctggacac gccgatgatg tttctcagga aggcctcgtt ctgccggttc 5101 cgggcgataa agtcggaggt ggcgatctcg cccacttgca gatagtagtt gtacttgccg 5161 cagttccgca cgtaggtgtc cagcttgttc agcagtttgt tttcgatcag ctttttcaga 5221 ttctggtact cgaagatccg cttgatctta tcgttgctga tgttgctcag ccgcttgtac 5281 acgtagtttt tcagcagctg ggacatctcg atttccacga agtggcagaa ggcgtactta 5341 atattcttgt cgttcagttc ctctttgtcc aggtagtact tgtagaacac ctggcttttc 5401 ttcagctcag acatgtcggg gatcttctca atcagctctt tgatgttgtt cacgttctgg 5461 atctcttcgt agataatctt ggcgaagttc tctttgtcgt tcttccggcc gatgatcttg 5521 tgatagtact cgcggatctt gtacttctcg tgcttcttgc tgttctcgat caggaaaaac 5581 agtttctcga tatcctcttt cttatacagc ttgtcgaagg cttcctgcac gttgttgatg 5641 tagtcgttgc gcttggcgct ctctctgtag tagtcgtaga tgatgttccg cttgctcttg 5701 ccgcccactt tttccacgtt gttctcgttg atcttctggt agttggcctt gttctcttcg 5761 aagctgtact tcaggctgtt gatcttgttc agcttggctt ccacgtcctt ccgaaagatt 5821 tccagttcct cagagttcac gtcctcgttc agcaggatct tcttcagcac ggagaagctg 5881 ttcttgtttt tcaggtcgta ctcgctgatg tcctcttcgc tatagttctt gtcctgcacg 5941 gcgttctttt ctttccggtt cttcagatac agcacgctgt ccttcaggtg cagcaccttg 6001 ttgctaaaga acttcttcag gttctctctt ctgatccggt tctcttcctc ggaggcgttg 6061 tcggggttct tgatgtagat gtccagccgg atgctcagca gctcgctcag tctctcgctg 6121 gtccggtttt cctcgctggt ggacttcacg agcttgccct cttcgatgta cttcttgtgg 6181 ctgatgccgt cgaccttggt cactttcatg gatccaccaa tctgttctct gtgagcctca 6241 ataatatcgt tatcctccat gtccaaatct tcaggggtct gatcagcttg gattctaata 6301 ccgtcgtaca agaatcttaa ggagtccatt tccttaccct gtcttttagc gaacgcttcc 6361 atcagccttc ttaaaggagt ggtctttttg atcttgaaga agatctctga agatccatcg 6421 gacaccttta aattgatgtg agtctcaggc ttgacttctg gcttgacctc tggcttagct 6481 tcttgattga cttctgagtc cgacattttc tcaaactgag ggtgtgacca tgcactacca 6541 cctgaaccac caccgctacc accacctttt tcaaactgcg gatggctcca gctagccata 6601 tggctgccgc gcggcaccag gccgctgctg tgatgatgat gatgatggct gctgcccatg 6661 gtatatctcc ttcttaaagt taaacaaaat tatttctaga ggggaattgt tatccgctca 6721 caattcccct atagtgagtc gtattaattt cgcgggatcg agatctcgat cctctacgcc 6781 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc 6841 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc 6901 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca 6961 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg 7021 caggagtcgc ataagggaga gcgtcgagat cccggacacc atcgaatggc gcaaaacctt 7081 tcgcggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga atgtgaaacc 7141 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt 7201 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc 7261 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct 7321 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat 7381 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg 7441 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat 7501 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt 7561 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca 7621 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc 7681 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa 7741 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat 7801 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct 7861 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg 7921 cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct catgttatat 7981 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg 8041 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact 8101 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 8161 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca 8221 acgcaattaa tgtaagttag ctcactcatt aggcaccggg atctcgaccg atgcccttga 8281 gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac 8341 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca 8401 ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat 8461 tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg 8521 gcgagaagca ggccattatc gccggcatgg cggccgacgc gctgggctac gtcttgctgg 8581 cgttcgcgac gcgaggctgg atggccttcc ccattatgat tcttctcgct tccggcggca 8641 tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt agatgacgac catcagggac 8701 agcttcaagg atcgctcgcg gctcttacca gcctaacttc gatcattgga ccgctgatcg 8761 tcacggcgat ttatgccgcc tcggcgagca catggaacgg gttggcatgg attgtaggcg 8821 ccgccctata ccttgtctgc ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct 8881 cgacctgaat ggaagccggc ggcacctcgc taacggattc accactccaa gaattggagc 8941 caatcaattc ttgcggagaa ctgtgaatgc gcaaaccaac ccttggcaga acatatccat 9001 cgcgtccgcc atctccagca gccgcacgcg gcgcatctcg ggcagcgttg ggtcctggcc 9061 acgggtgcgc atgatcgtgc tcctgtcgtt gaggacccgg ctaggctggc ggggttgcct 9121 tactggttag cagaatgaat caccgatacg cgagcgaacg tgaagcgact gctgctgcaa 9181 aacgtctgcg acctgagcaa caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct 9241 ggaaacgcgg aagtcagcgc cctgcaccat tatgttccgg atctgcatcg caggatgctg 9301 ctggctaccc tgtggaacac ctacatctgt attaacgaag cgctggcatt gaccctgagt 9361 gatttttctc tggtcccgcc gcatccatac cgccagttgt ttaccctcac aacgttccag 9421 taaccgggca tgttcatcat cagtaacccg tatcgtgagc atcctctctc gtttcatcgg 9481 tatcattacc cccatgaaca gaaatccccc ttacacggag gcatcagtga ccaaacagga 9541 aa //