LOCUS Exported 1966 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Linker to construct BB1 from PCR product with FS3 and FS4 (in with BsaI, out with BbsI). ACCESSION . VERSION . KEYWORDS BB1_L_34_syn_BsaI SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1966) AUTHORS Sarkari P, Marx H, Blumhoff ML, Mattanovich D, Sauer M, Steiger MG TITLE An efficient tool for metabolic pathway construction and gene integration for Aspergillus niger. JOURNAL Bioresour Technol. 2017 May 4. pii: S0960-8524(17)30643-0. doi: 10.1016/j.biortech.2017.05.004. PUBMED 28533066 REFERENCE 2 (bases 1 to 1966) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1966 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(41..57) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(41..57) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" terminator 105..136 /label=tonB terminator /note="bidirectional E. coli tonB-P14 transcription terminator" promoter 137..239 /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" CDS 240..1055 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(313..332) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" terminator 1079..1106 /label=T7Te terminator /note="phage T7 early transcription terminator" rep_origin complement(1118..1705) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1198..1217) /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 1745..1762 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 1746..1762 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 gatcggtcca cgatcagcta gattatctag tcagcttgat gtcatagctg tttcctgagg 61 ctcaatactg accatttaaa tcatacctga cctccatagc agaaagtcaa aagcctccga 121 ccggaggctt ttgacttgat cggcacgtaa gaggttccaa ctttcaccat aatgaaataa 181 gatcactacc gggcgtattt tttgagttat cgagattttc aggagctaag gaagctaaaa 241 tgagccatat tcaacgggaa acgtcttgct tgaagccgcg attaaattcc aacatggatg 301 ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct 361 atcgattgta tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg 421 ttgccaatga tgttacagat gagatggtca ggctaaactg gctgacggaa tttatgcctc 481 ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga 541 tcccagggaa aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg 601 ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt 661 ttaacggcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg 721 ttggtgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag 781 aaatgcataa actcttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac 841 ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg 901 gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc 961 cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat 1021 tgcagtttca cttgatgctc gatgagtttt tctaatgagg acctaaatgt aatcacctgg 1081 ctcaccttcg ggtgggcctt tctgcgttgc tggcgttttt ccataggctc cgcccccctg 1141 acgagcatca caaaaatcga tgctcaagtc agaggtggcg aaacccgaca ggactataaa 1201 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 1261 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 1321 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 1381 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 1441 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 1501 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa 1561 cagtatttgg tatctgcgct ctgctgaagc cagttacctc ggaaaaagag ttggtagctc 1621 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 1681 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatt ttctaccgaa gaaaggccca 1741 cccgtgtaaa acgacggcca gtttatctag tcagcttgat tctagctgat cgtggaccgg 1801 aaggtgagcc agtgagttga ttgcagtcca gttacgctgg agtctgaggc tcgtcctgaa 1861 tgatatgcgg cctcgaattc gaagacgcgc tttgagaccg gatccatctc gagggtctca 1921 cgctgcgtct tcgaattcgc gcgtgatctt acggcattat acgtat //