LOCUS       Exported                 782 bp ds-DNA     linear   SYN 25-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    PSUPER-1660s(hPot1)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Veldman T, Etheridge KT, Counter CM
  TITLE     Loss of hPot1 function leads to telomere instability and a cut-like 
            phenotype.
  JOURNAL   Curr Biol. 2004 Dec 29. 14(24):2264-70.
  PUBMED    15620654
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 25, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     39..55
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     40..56
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(105..319)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(165..184)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(339..355)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(339..355)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(387..406)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(388..406)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(413..430)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(413..429)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(422..444)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(658..677)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cctcactaaa gggacaaaag ctggtaccgg gccccccctc gaggtcgacg gtatcgataa
       61 gcttttccaa aagtactaga agcctatctc atctcttggg atctgtggtc tcatacagaa
      121 cttataagat tcccaaatcc aaagacattt cacgtttatg gtgatttccc agaacacata
      181 gcgacatgca aatattgcag ggcgccactc ccctgtccct cacagccatc ttcctgccag
      241 ggcgcacgcg cgctgggtgt tcccgcctag tgacactggg cccgcgattc cttggagcgg
      301 gttgatgacg tcagcgttcg aattcctgca gcccggggga tccactagtt ctagagcggc
      361 cgccaccgcg gtggagctcc aattcgccct atagtgagtc gtattacaat tcactggccg
      421 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
      481 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
      541 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
      601 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
      661 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgc aagctctaaa
      721 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
      781 tg
//
