LOCUS       Exported               11526 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Retroviral overexpression vector (doxycycline-inducible) for Tau.
ACCESSION   .
VERSION     .
KEYWORDS    pINDUCER20-Tau
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11526)
  AUTHORS   Rousseaux MW, de Haro M, Lasagna-Reeves CA, De Maio A, Park J, 
            Jafar-Nejad P, Al-Ramahi I, Sharma A, See L, Lu N, Vilanova-Velez L,
            Klisch TJ, Westbrook TF, Troncoso JC, Botas J, Zoghbi HY
  TITLE     TRIM28 regulates the nuclear accumulation and toxicity of both 
            alpha-synuclein and tau.
  JOURNAL   Elife. 2016 Oct 25;5. pii: e19809. doi: 10.7554/eLife.19809.
  PUBMED    27779468
REFERENCE   2  (bases 1 to 11526)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11526
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2230..2500
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2533..2571
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2568..2592
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2701..2725
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4064..4093
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    complement(4113..4137)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4190..4216
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        4302..5513
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5350..5369
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             5572..6318
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6356..6929
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6523..6540)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6750..6769
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6945..7739
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6999..7018)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7609..7628
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    7916..8504
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7969..7989)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8387..8398)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8579..8812
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8931..8949)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9017..9121
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9122..9982
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9340..9359)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10153..10741
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10642..10661
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10895..10912
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     11004..11023
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     protein_bind    11152..11173
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        11188..11218
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11226..11242
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11231..11253
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11250..11266
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11250..11266
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11316..11335
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactaggatc
     2221 ctttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     2281 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     2341 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     2401 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     2461 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagctcgg
     2521 tacccgggtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
     2581 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
     2641 gaccgatcca gcctccgcgg ccccgaacta gtccagtgtg gtggaattct gcagatatca
     2701 acaagtttgt acaaaaaagc aggctaagct tgctcgaggg atggctgagc cccgccagga
     2761 gttcgaagtg atggaagatc acgctgggac gtacgggttg ggggacagga aagatcaggg
     2821 gggctacacc atgcaccaag accaagaggg tgacacggac gctggcctga aagaatctcc
     2881 cctgcagacc cccactgagg acggatctga ggaaccgggc tctgaaacct ctgatgctaa
     2941 gagcactcca acagcggaag atgtgacagc acccttagtg gatgagggag ctcccggcaa
     3001 gcaggctgcc gcgcagcccc acacggagat cccagaagga accacagctg aagaagcagg
     3061 cattggagac acccccagcc tggaagacga agctgctggt cacgtgaccc aagctcgcat
     3121 ggtcagtaaa agcaaagacg ggactggaag cgatgacaaa aaagccaagg gggctgatgg
     3181 taaaacgaag atcgccacac cgcggggagc agcccctcca ggccagaagg gccaggccaa
     3241 cgccaccagg attccagcaa aaaccccgcc cgctccaaag acaccaccca gctctggtga
     3301 acctccaaaa tcaggggatc gcagcggcta cagcagcccc ggctccccag gcactcccgg
     3361 cagccgctcc cgcaccccgt cccttccaac cccacccacc cgggagccca agaaggtggc
     3421 agtggtccgt actccaccca agtcgccgtc ttccgccaag agccgcctgc agacagcccc
     3481 cgtgcccatg ccagacctga agaatgtcaa gtccaagatc ggctccactg agaacctgaa
     3541 gcaccagccg ggaggcggga aggtgcagat aattaataag aagctggatc ttagcaacgt
     3601 ccagtccaag tgtggctcaa aggataatat caaacacgtc ccgggaggcg gcagtgtgca
     3661 aatagtctac aaaccagttg acctgagcaa ggtgacctcc aagtgtggct cattaggcaa
     3721 catccatcat aaaccaggag gtggccaggt ggaagtaaaa tctgagaagc ttgacttcaa
     3781 ggacagagtc cagtcgaaga ttgggtccct ggacaatatc acccacgtcc ctggcggagg
     3841 aaataaaaag attgaaaccc acaagctgac cttccgcgag aacgccaaag ccaagacaga
     3901 ccacggggcg gagatcgtgt acaagtcgcc agtggtgtct ggggacacgt ctccacggca
     3961 tctcagcaat gtctcctcca ccggcagcat cgacatggta gactcgcccc agctcgccac
     4021 actagctgac gaggtgtctg cctccctggc caagcagggt ttggaacaaa aactcatctc
     4081 agaagaggat ctgtgagccc ggtgcggccg caacccagct ttcttgtaca aagtggttga
     4141 tatccagcac agtggcggcc gctcgagtct agagggcccg cggttcgaat acccatacga
     4201 cgtcccagac tacgcttagt aatgattaat taaactagaa attctaccgg gtaggggagg
     4261 cgcttttccc aaggcagtct ggagtaaccc acccaagatc tggcctccgc gccgggtttt
     4321 ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag acgaagggcg
     4381 cagcgagcgt cctgatcctt ccgcccggac gctcaggaca gcggcccgct gctcataaga
     4441 ctcggcctta gaaccccagt atcagcagaa ggacatttta ggacgggact tgggtgactc
     4501 tagggcactg gttttctttc cagagagcgg aacaggcgag gaaaagtagt cccttctcgg
     4561 cgattctgcg gagggatctc cgtggggcgg tgaacgccga tgattatata aggacgcgcc
     4621 gggtgtggca cagctagttc cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga
     4681 tcgctgtgat cgtcacttgg tgagtagcgg gctgctgggc tggccggggc tttcgtggcc
     4741 gccgggccgc tcggtgggac ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt
     4801 ccgcgagcaa ggttgccctg aactgggggt tggggggagc gcagcaaaat ggcggctgtt
     4861 cccgagtctt gaatggaaga cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg
     4921 ggggcatggt gggcggcaag aacccaaggt cttgaggcct tcgctaatgc gggaaagctc
     4981 ttattcgggt gagatgggct ggggcaccat ctggggaccc tgacgtgaag tttgtcactg
     5041 actggagaac tcggtttgtc gtctgttgcg ggggcggcag ttatggcggt gccgttgggc
     5101 agtgcacccg tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt
     5161 tggcttataa tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct tttctccgtc
     5221 gcaggacgca gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg ccggacctct
     5281 ggtgagggga gggataagtg aggcgtcagt ttctttggtc ggttttatgt acctatcttc
     5341 ttaagtagct gaagctccgg ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg
     5401 aagtttttta ggcacctttt gaaatgtaat catttgggtc aatatgtaat tttcagtgtt
     5461 agactagtaa attgtccgct aaattctggc cgtttttggc ttttttgtta gacgaagctt
     5521 ggtaccgagc tcggatctcc accccgtacc ggtcctgcag tcgaattcac catgtctaga
     5581 ctggacaaga gcaaagtcat aaacggagct ctggaattac tcaatggtgt cggtatcgaa
     5641 ggcctgacga caaggaaact cgctcaaaag ctgggagttg agcagcctac cctgtactgg
     5701 cacgtgaaga acaagcgggc cctgctcgat gccctgccaa tcgagatgct ggacaggcat
     5761 catacccact tctgccccct ggaaggcgag tcatggcaag actttctgcg gaacaacgcc
     5821 aagtcatacc gctgtgctct cctctcacat cgcgacgggg ctaaagtgca tctcggcacc
     5881 cgcccaacag agaaacagta cgaaaccctg gaaaatcagc tcgcgttcct gtgtcagcaa
     5941 ggcttctccc tggagaacgc actgtacgct ctgtccgccg tgggccactt tacactgggc
     6001 tgcgtattgg aggaacagga gcatcaagta gcaaaagagg aaagagagac acctaccacc
     6061 gattctatgc ccccacttct gagacaagca attgagctgt tcgaccggca gggagccgaa
     6121 cctgccttcc ttttcggcct ggaactaatc atatgtggcc tggagaaaca gctaaagtgc
     6181 gaaagcggcg ggccgaccga cgcccttgac gattttgact tagacatgct cccagccgat
     6241 gcccttgacg actttgacct tgatatgctg cctgctgacg ctcttgacga ttttgacctt
     6301 gacatgctcc ccgggtaact aagtaaggat ccgcggccgc actagaggaa ttccgcccct
     6361 ctcccccccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
     6421 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
     6481 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
     6541 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
     6601 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
     6661 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
     6721 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
     6781 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat
     6841 gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg
     6901 tggttttcct ttgaaaaaca cgatgataag cttaccggtc caccatgatt gaacaagatg
     6961 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     7021 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     7081 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc
     7141 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     7201 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
     7261 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     7321 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     7381 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
     7441 cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg
     7501 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     7561 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     7621 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     7681 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgat
     7741 gtacaagtaa agcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt
     7801 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     7861 attgttgttg tttagtccct cccaattcga tatcaagctt atcgcgatag atcctaatca
     7921 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     7981 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     8041 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     8101 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     8161 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     8221 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     8281 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     8341 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     8401 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     8461 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctgaga tcctttaaga
     8521 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     8581 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     8641 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     8701 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     8761 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     8821 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     8881 aggcccgggt taattaagga aagggctaga tcattcttga agacgaaagg gcctcgtgat
     8941 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     9001 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     9061 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     9121 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     9181 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     9241 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     9301 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     9361 ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     9421 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     9481 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     9541 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     9601 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     9661 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     9721 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     9781 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     9841 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     9901 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     9961 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
    10021 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
    10081 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
    10141 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
    10201 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
    10261 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
    10321 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
    10381 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
    10441 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
    10501 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
    10561 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
    10621 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
    10681 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
    10741 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
    10801 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
    10861 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
    10921 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagcaa
    10981 gctcatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
    11041 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccc
    11101 gtggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
    11161 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
    11221 tggaattgtg agcggataac aatttcacac aggaaacagc tatgacatga ttacgaattt
    11281 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
    11341 atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc
    11401 caccccatac cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga
    11461 ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt
    11521 agtagt
//
