LOCUS Exported 5729 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Bacterial expression of a fluorescent protein, Infrared Fluorescent Protein 1.4 + Heme oxygenase-1 (IFP1.4). ACCESSION . VERSION . KEYWORDS pBAD IFP1.4 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5729) AUTHORS Shu X, Royant A, Lin MZ, Aguilera TA, Lev-Ram V, Steinbach PA, Tsien RY TITLE Mammalian expression of infrared fluorescent proteins engineered from a bacterial phytochrome. JOURNAL Science. 2009 May 8;324(5928):804-7. doi: 10.1126/science.1168683. PUBMED 19423828 REFERENCE 2 (bases 1 to 5729) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5729 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 19..303 /gene="araBAD" /label=araBAD promoter /note="promoter of the L-arabinose operon of E. coli; the araC regulatory gene is transcribed in the opposite direction (Guzman et al., 1995)" primer_bind 226..245 /label=pBAD Forward /note="For vectors with E. coli araBAD promoter, forward primer" RBS 330..352 /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" CDS 1331..1348 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" primer_bind complement(2148..2165) /label=pBAD Reverse /note="For vectors with E. coli araBAD promoter, reverse primer" terminator 2318..2404 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 2496..2523 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" promoter 2542..2633 /gene="bla" /label=AmpR promoter CDS 2634..3494 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(2852..2871) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 3665..4253 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4154..4173 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 4407..4424 /label=L4440 /note="L4440 vector, forward primer" misc_feature 4439..4579 /label=bom /note="basis of mobility region from pBR322" primer_bind complement(4505..4524) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 4665..4687 /label=pGEX 3' /note="pGEX vectors, reverse primer" CDS complement(4843..5721) /codon_start=1 /gene="araC" /product="L-arabinose regulatory protein" /label=araC /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE EKVNDVAVKLS" ORIGIN 1 tactcccgcc attcagagaa gaaaccaatt gtccatattg catcagacat tgccgtcact 61 gcgtctttta ctggctgttc tcgctaacca aaccggtaac cccgcttatt aaaagcattc 121 tgtaacaaag cgggaccaaa gccatgacaa aaacgcgtaa caaaagtgtc tataatcacg 181 gcagaaaagt ccacattgat tatttgcacg gcgtcacact ttgctatgcc atagcatttt 241 tatccataag attagccgat cctacctgac gctttttatc gcaactctct actgtttctc 301 gatacccgtt tttttgggct agaaataatt ttgtttaact ttaagaagga gatatggatc 361 catggctcgt gatccgctgc cgtttttccc gcccctgtat ctgggtggtc cggagatcac 421 cactgaaaac tgtgagcgtg aaccgattca tatcccgggt tccatccagc cgcacggtgc 481 actgctgacc gcagatggcc actctggcga agtgctgcag gtgtctctga acgctgcaac 541 tttcctgggt caggagccga ccgttctgcg tggccagacc ctggcggctc tgctgccgga 601 acagtggccg gctctgcagg cggctctgcc gccgggttgc ccggatgcgc tgcagtaccg 661 cgctaccctg gattggccgg cagctggtca cctgtccctg accgttcacc gtgtggctga 721 actgttgatc ctggaatttg aaccgaccga agcatgggat tccatcggcc cgcacgcact 781 gcgtaacgct atgtttgcac tggaatccgc accgaacctg cgtgctctgg cggaggttgc 841 tactcagacc gttcgcgaac tgactggttt tgatcgcgta atgctgtaca agttcgcacc 901 ggatgcgact ggtgaaatga tcgcggaagc tcgtcgcgaa ggtatgcaag cttttctggg 961 tcaccgtttc ccggctagcc acaccccggc gcaggctcgc gcactgtaca ctcgtcacct 1021 gctgcgtctg actgcggaca cccgcgcagc tgcggttccg ctggacccgg ttctgaaccc 1081 gcagaccaac gcgccgaccc cgctgggcgg cgcggttctg cgtgcgacca gcccgatgca 1141 tatgcagtac ctgcgtaaca tgggtgttgg cagctccctg tctgtgagcg tggtagttgg 1201 cggtcagctg tggggtctga tcgtgtgtca ccaccagact ccgtacgttc tgccgccgga 1261 tctgcgcacc accctggagg agctgggtcg taagctgagc ggccaggtac agcgcaaaga 1321 ggcggaattc catcatcacc atcaccattg aaagaaggag atatacatac ccatgagtgt 1381 caacttagct tcccagttgc gggaagggac gaaaaaatcc cactccatgg cggagaacgt 1441 cggctttgtc aaatgcttcc tcaagggcgt tgtcgagaaa aattcctacc gtaagctggt 1501 tggcaatctc tactttgtct acagtgccat ggaagaggaa atggcaaaat ttaaggacca 1561 tcccatcctc agccacattt acttccccga actcaaccgc aaacaaagcc tagagcaaga 1621 cctgcaattc tattacggct ccaactggcg gcaagaagtg aaaatttctg ccgctggcca 1681 agcctatgtg gaccgagtcc ggcaagtggc cgctacggcc cctgaattgt tggtggccca 1741 ttcctacacc cgttacctgg gggatctttc cggcggtcaa attctcaaga aaattgccca 1801 aaatgccatg aatctccacg atggtggcac agctttctat gaatttgccg acattgatga 1861 cgaaaaggct tttaaaaata cctaccgtca agctatgaat gatctgccca ttgaccaagc 1921 caccgccgaa cggattgtgg atgaagccaa tgacgccttt gccatgaaca tgaaaatgtt 1981 caacgaactt gaaggcaacc tgatcaaggc gatcggcatt atggtgttca acagcctcac 2041 ccgtcgccgc agtcaaggca gcaccgaagt tggcctcgcc acctccgaag gctaggttta 2101 aacggtctcc agcttggctg ttttggcgga tgagagaaga ttttcagcct gatacagatt 2161 aaatcagaac gcagaagcgg tctgataaaa cagaatttgc ctggcggcag tagcgcggtg 2221 gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg 2281 gggtctcccc atgcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc 2341 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac 2401 aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg 2461 acgcccgcca taaactgcca ggcatcaaat taagcagaag gccatcctga cggatggcct 2521 ttttgcgttt ctacaaactc ttttgtttat ttttctaaat acattcaaat atgtatccgc 2581 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta 2641 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg 2701 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg 2761 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac 2821 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg 2881 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt 2941 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg 3001 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac 3061 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt 3121 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag 3181 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc 3241 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc 3301 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta 3361 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg 3421 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga 3481 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac 3541 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa 3601 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat 3661 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc 3721 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg 3781 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc 3841 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg 3901 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg 3961 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa 4021 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg 4081 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga 4141 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct 4201 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca 4261 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc 4321 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg 4381 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gtagagcgcc 4441 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgtata tggtgcactc 4501 tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg 4561 tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc 4621 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg 4681 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag cagatcaatt cgcgcgcgaa 4741 ggcgaagcgg catgcataat gtgcctgtca aatggacgaa gcagggattc tgcaaaccct 4801 atgctactcc gtcaagccgt caattgtctg attcgttacc aattatgaca acttgacggc 4861 tacatcattc actttttctt cacaaccggc acggaactcg ctcgggctgg ccccggtgca 4921 ttttttaaat acccgcgaga aatagagttg atcgtcaaaa ccaacattgc gaccgacggt 4981 ggcgataggc atccgggtgg tgctcaaaag cagcttcgcc tggctgatac gttggtcctc 5041 gcgccagctt aagacgctaa tccctaactg ctggcggaaa agatgtgaca gacgcgacgg 5101 cgacaagcaa acatgctgtg cgacgctggc gatatcaaaa ttgctgtctg ccaggtgatc 5161 gctgatgtac tgacaagcct cgcgtacccg attatccatc ggtggatgga gcgactcgtt 5221 aatcgcttcc atgcgccgca gtaacaattg ctcaagcaga tttatcgcca gcagctccga 5281 atagcgccct tccccttgcc cggcgttaat gatttgccca aacaggtcgc tgaaatgcgg 5341 ctggtgcgct tcatccgggc gaaagaaccc cgtattggca aatattgacg gccagttaag 5401 ccattcatgc cagtaggcgc gcggacgaaa gtaaacccac tggtgatacc attcgcgagc 5461 ctccggatga cgaccgtagt gatgaatctc tcctggcggg aacagcaaaa tatcacccgg 5521 tcggcaaaca aattctcgtc cctgattttt caccaccccc tgaccgcgaa tggtgagatt 5581 gagaatataa cctttcattc ccagcggtcg gtcgataaaa aaatcgagat aaccgttggc 5641 ctcaatcggc gttaaacccg ccaccagatg ggcattaaac gagtatcccg gcagcagggg 5701 atcattttgc gcttcagcca tacttttca //