LOCUS Exported 10589 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Yeast two hybrid. ACCESSION . VERSION . KEYWORDS pGAD-GH-MEFV SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10589) AUTHORS Gao W, Yang J, Liu W, Wang Y, Shao F TITLE Site-specific phosphorylation and microtubule dynamics control Pyrin inflammasome activation. JOURNAL Proc Natl Acad Sci U S A. 2016 Aug 16;113(33):E4857-66. doi: 10.1073/pnas.1601700113. Epub 2016 Aug 1. PUBMED 27482109 REFERENCE 2 (bases 1 to 10589) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10589 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 1..705 /gene="S. cerevisiae ADH1" /label=ADH1 promoter /note="promoter for alcohol dehydrogenase 1" CDS 751..771 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 787..1128 /codon_start=1 /gene="Saccharomyces cerevisiae GAL4 (truncated)" /product="activation domain of the GAL4 transcriptional activator" /label=GAL4 activation domain /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD EDTPPNPKKE" promoter complement(3538..3555) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" polyA_signal 3560..3694 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3584..3603) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 3638..3657 /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind complement(3802..3821) /label=T7 /note="T7 promoter, forward primer" promoter complement(3803..3821) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(3828..3845) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(3828..3844) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3837..3859) /label=M13/pUC Forward /note="In lacZ gene" terminator 4247..4434 /gene="S. cerevisiae ADH1" /label=ADH1 terminator /note="transcription terminator for the S. cerevisiae alcohol dehydrogenase 1 (ADH1) gene" primer_bind complement(4302..4326) /label=pHybLex-R /note="ADH terminator, reverse primer" CDS complement(4551..5645) /codon_start=1 /gene="S. cerevisiae LEU2" /product="3-isopropylmalate dehydrogenase, required for leucine biosynthesis" /label=LEU2 /note="yeast auxotrophic marker" /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG DLGGSNSTTEVGDAVAEEVKKILA" promoter complement(5646..6051) /gene="S. cerevisiae LEU2" /label=LEU2 promoter primer_bind complement(6093..6109) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(6093..6109) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(6106..6128) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 6117..6133 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(6141..6171) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6186..6207 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." protein_bind complement(6262..6295) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." protein_bind complement(6373..6406) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." primer_bind complement(6475..6492) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(6646..7234) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(6726..6745) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(7405..8265) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 8028..8047 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(8266..8370) /gene="bla" /label=AmpR promoter primer_bind 8438..8456 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(8494..8516) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 8616..8635 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" rep_origin 8652..9816 /label=2u ori /note="yeast 2u plasmid origin of replication" ORIGIN 1 atccttttgt tgtttccggg tgtacaatat ggacttcctc ttttctggca accaaaccca 61 tacatcggga ttcctataat accttcgttg gtctccctaa catgtaggtg gcggagggga 121 gatatacaat agaacagata ccagacaaga cataatgggc taaacaagac tacaccaatt 181 acactgcctc attgatggtg gtacataacg aactaatact gtagccctag acttgatagc 241 catcatcata tcgaagtttc actacccttt ttccatttgc catctattga agtaataata 301 ggcgcatgca acttcttttc tttttttttc ttttctctct cccccgttgt tgtctcacca 361 tatccgcaat gacaaaaaaa tgatggaaga cactaaagga aaaaattaac gacaaagaca 421 gcaccaacag atgtcgttgt tccagagctg atgaggggta tctcgaagca cacgaaactt 481 tttccttcct tcattcacgc acactactct ctaatgagca acggtatacg gccttccttc 541 cagttacttg aatttgaaat aaaaaaaagt ttgctgtctt gctatcaagt ataaatagac 601 ctgcaattat taatcttttg tttcctcgtc attgttctcg ttccctttct tccttgtttc 661 tttttctgca caatatttca agctatacca agcatacaat caactccaag ctttgcaaag 721 atggataaag cggaattaat tcccgagcct ccaaaaaaga agagaaaggt cgaattgggt 781 accgccgcca attttaatca aagtgggaat attgctgata gctcattgtc cttcactttc 841 actaacagta gcaacggtcc gaacctcata acaactcaaa caaattctca agcgctttca 901 caaccaattg cctcctctaa cgttcatgat aacttcatga ataatgaaat cacggctagt 961 aaaattgatg atggtaataa ttcaaaacca ctgtcacctg gttggacgga ccaaactgcg 1021 tataacgcgt ttggaatcac tacagggatg tttaatacca ctacaatgga tgatgtatat 1081 aactatctat tcgatgatga agatacccca ccaaacccaa aaaaagagat cctagaacta 1141 gtggatcccc cgggctgcag gaattcaggc cggccaatgg ctaagacccc tagtgaccat 1201 ctgctgtcca ccctggagga gctggtgccc tatgacttcg agaagttcaa gttcaagctg 1261 cagaacacca gtgtgcagaa ggagcactcc aggatccccc ggagccagat ccagagagcc 1321 aggccggtga agatggccac tctgctggtc acctactatg gggaagagta cgccgtgcag 1381 ctcaccctgc aggtcctgcg ggccatcaac cagcgcctgc tggccgagga gctccacagg 1441 gcagccattc aggaatattc cacacaagaa aacggcacag atgattccgc agcgtccagc 1501 tccctggggg agaacaagcc caggagcctg aagactccag accaccccga ggggaacgag 1561 gggaacggcc ctcggccgta cgggggcgga gctgccagcc tgcggtgcag ccagcccgag 1621 gccgggaggg ggctgtcgag gaagcccctg agcaaacgca gagagaaggc ctcggagggc 1681 ctggacgcgc agggcaagcc tcggacccgg agcccggccc tgccgggcgg gagaagcccc 1741 ggcccctgca gggcgctaga ggggggccag gccgaggtcc ggctgcgcag aaacgccagc 1801 tccgcgggga ggctgcaggg gctggcgggg ggcgccccgg ggcagaagga gtgcaggccc 1861 ttcgaagtgt acctgccctc gggaaagatg cgacctagaa gccttgaggt caccatttct 1921 acaggggaga aggcgcccgc aaatccagaa attctcctga ctctagagga aaagacagct 1981 gcgaatctgg actcggcaac agaaccccgg gcaaggccca ctccggatgg aggggcatct 2041 gcggacctga aggaaggccc tggaaatcca gaacattcgg tcaccggaag gccaccagac 2101 acggctgcga gtccccgttg ccacgcccag gaaggagacc cagttgacgg tacctgtgtg 2161 cgtgattcct gcagcttccc cgaggcagtt tctgggcacc cccaggcctc aggcagccgc 2221 tcacctggct gcccccggtg ccaggactcc catgaaagga agagcccggg aagcctaagc 2281 ccccagcccc tgccacagtg taagcgccac ctgaagcagg tccagctgct cttctgtgag 2341 gatcacgatg agcccatctg cctcatctgc agtctgagtc aggagcacca aggccaccgg 2401 gtgcgcccca ttgaggaggt cgccctggaa cacaagaaga aaattcagaa gcagctggag 2461 catctgaaga agctgagaaa atcaggggag gagcagcgat cctatgggga ggagaaggca 2521 gtgagctttc tgaaacaaac tgaagcgctg aagcagcggg tgcagaggaa gctggagcag 2581 gtgtactact tcctggaaca gcaggagcat ttctttgtgg cctcactgga ggacgtgggc 2641 cagatggttg ggcagatcag gaaggcatat gacacccgcg tatcccagga catcgccctg 2701 ctcgacgcgc tgattgggga actggaggcc aaggagtgcc agtcagaatg ggaacttctg 2761 caggacattg gagacatctt gcacagggct aagacagtgc ctgtccctga aaagtggacc 2821 actcctcaag agataaaaca aaagatccaa ctcctccacc agaagtcaga gtttgtggag 2881 aagagcacaa agtacttctc agaaaccctg cgttcagaaa tggaaatgtt caatgttccg 2941 gagctgattg gcgctcaggc acatgctgtt aatgtgattc tggatgcaga aaccgcttac 3001 cccaacctca tcttctctga tgatctgaag agtgttagac ttggaaacaa gtgggagagg 3061 ctgcctgatg gcccgcaaag atttgacagc tgtatcattg ttctgggctc tccgagtttc 3121 ctctctggcc gccgttactg ggaggtggag gttggagaca agacagcatg gatcctggga 3181 gcctgcaaga catccataag caggaaaggg aacatgactc tgtcgccaga gaatggctac 3241 tgggtggtga taatgatgaa ggaaaatgag taccaggcgt ccagcgttcc cccgacccgc 3301 ctgctaataa aggagcctcc caagcgtgtg ggcatcttcg tggactacag agttggaagc 3361 atctcctttt acaatgtgac agccagatcc cacatctata cattcgccag ctgctctttc 3421 tctgggcccc ttcaacctat cttcagccct gggacacgtg atggagggaa gaacacagct 3481 cctctgacta tctgtccagt gggtggtcag gggcctgact gaggcgcgcc tctagaacta 3541 tagtgagtcg tattacgtag atccagacat gataagatac attgatgagt ttggacaaac 3601 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt 3661 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat 3721 gtttcaggtt cagggggagg tgtgggaggt tttttaattc gcggccgcgg cgccaatgca 3781 ttgggcccgg tacccaattc gccctatagt gagtcgtatt acaattcact ggccgtcgtt 3841 ttacaacgtc gtgactggga aaaccctgat ctatgaatcg tagatactga aaaaccccgc 3901 aagttcactt caactgtgca tcgtgcacca tctcaatttc tttcatttat acatcgtttt 3961 gccttctttt atgtaactat actcctctaa gtttcaatct tggccatgta acctctgatc 4021 tatagaattt tttaaatgac tagaattaat gcccatcttt tttttggacc taaattcttc 4081 atgaaaatat attacgaggg cttattcaga agctttggac ttcttcgcca gaggtttggt 4141 caagtctcca atcaaggttg tcggcttgtc taccttgcca gaaatttacg aaaagatgga 4201 aaagggtcaa atcgttggta gatacgttgt tgacacttct aaataagcga atttcttatg 4261 atttatgatt tttattatta aataagttat aaaaaaaata agtgtataca aattttaaag 4321 tgactcttag gttttaaaac gaaaattctt attcttgagt aactctttcc tgtaggtcag 4381 gttgctttct caggtatagc atgaggtcgc tcttattgac cacacctcta ccggccggtc 4441 gaaattcccc taccctatga acatattcca ttttgtaatt tcgtgtcgtt tctattatga 4501 atttcattta taaagtttat gtacaaatat cataaaaaaa gagaatcttt ttaagcaagg 4561 attttcttaa cttcttcggc gacagcatca ccgacttcgg tggtactgtt ggaaccacct 4621 aaatcaccag ttctgatacc tgcatccaaa acctttttaa ctgcatcttc aatggcctta 4681 ccttcttcag gcaagttcaa tgacaatttc aacatcattg cagcagacaa gatagtggcg 4741 atagggttga ccttattctt tggcaaatct ggagcagaac cgtggcatgg ttcgtacaaa 4801 ccaaatgcgg tgttcttgtc tggcaaagag gccaaggacg cagatggcaa caaacccaag 4861 gaacctggga taacggaggc ttcatcggag atgatatcac caaacatgtt gctggtgatt 4921 ataataccat ttaggtgggt tgggttctta actaggatca tggcggcaga atcaatcaat 4981 tgatgttgaa ccttcaatgt aggaaattcg ttcttgatgg tttcctccac agtttttctc 5041 cataatcttg aagaggccaa aacattagct ttatccaagg accaaatagg caatggtggc 5101 tcatgttgta gggccatgaa agcggccatt cttgtgattc tttgcacttc tggaacggtg 5161 tattgttcac tatcccaagc gacaccatca ccatcgtctt cctttctctt accaaagtaa 5221 atacctccca ctaattctct gacaacaacg aagtcagtac ctttagcaaa ttgtggcttg 5281 attggagata agtctaaaag agagtcggat gcaaagttac atggtcttaa gttggcgtac 5341 aattgaagtt ctttacggat ttttagtaaa ccttgttcag gtctaacact acctgtaccc 5401 catttaggac cacccacagc acctaacaaa acggcatcag ccttcttgga ggcttccagc 5461 gcctcatctg gaagtggaac acctgtagca tcgatagcag caccaccaat taaatgattt 5521 tcgaaatcga acttgacatt ggaacgaaca tcagaaatag ctttaagaac cttaatggct 5581 tcggctgtga tttcttgacc aacgtggtca cctggcaaaa cgacgatctt cttaggggca 5641 gacattagaa tggtatatcc ttgaaatata tatatatatt gctgaaatgt aaaaggtaag 5701 aaaagttaga aagtaagacg attgctaacc acctattgga aaaaacaata ggtccttaaa 5761 taatattgtc aacttcaagt attgtgatgc aagcatttag tcatgaacgc ttctctattc 5821 tatatgaaaa gccggttccg gcgctctcac ctttcctttt tctcccaatt tttcagttga 5881 aaaaggtata tgcgtcaggc gacctctgaa attaacaaaa aatttccagt catcgaattt 5941 gattctgtgc gatagcgccc ctgtgtgttc tcgttatgtt gaggaaaaaa ataatggttg 6001 ctaagagatt cgaactcttg catcttacga tacctgagta ttcccacagt tgggggatct 6061 cgactctagc tagaggatca attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt 6121 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt 6181 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg 6241 ggaaacctgt cgtgccagct gataacttcg tataatgtat gctatacgaa gttattaggt 6301 ctgaagagga gtttacgtcc agccaagcta gcttggctgc aggtcgagcg gccgcgatcc 6361 ggaaccctta atataacttc gtataatgta tgctatacga agttatcagc tgcattaatg 6421 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct 6481 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 6541 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 6601 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 6661 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 6721 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 6781 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 6841 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 6901 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 6961 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 7021 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 7081 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 7141 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 7201 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 7261 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 7321 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 7381 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 7441 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 7501 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 7561 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 7621 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 7681 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 7741 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 7801 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 7861 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 7921 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 7981 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 8041 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 8101 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 8161 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 8221 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 8281 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 8341 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt 8401 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt 8461 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 8521 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 8581 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag 8641 agtgcaccat aacgcattta agcataaaca cgcactatgc cgttcttctc atgtatatat 8701 atatacaggc aacacgcaga tataggtgcg acgtgaacag tgagctgtat gtgcgcagct 8761 cgcgttgcat tttcggaagc gctcgttttc ggaaacgctt tgaagttcct attccgaagt 8821 tcctattctc tagctagaaa gtataggaac ttcagagcgc ttttgaaaac caaaagcgct 8881 ctgaagacgc actttcaaaa aaccaaaaac gcaccggact gtaacgagct actaaaatat 8941 tgcgaatacc gcttccacaa acattgctca aaagtatctc tttgctatat atctctgtgc 9001 tatatcccta tataacctac ccatccacct ttcgctcctt gaacttgcat ctaaactcga 9061 cctctacatt ttttatgttt atctctagta ttactcttta gacaaaaaaa ttgtagtaag 9121 aactattcat agagtgaatc gaaaacaata cgaaaatgta aacatttcct atacgtagta 9181 tatagagaca aaatagaaga aaccgttcat aattttctga ccaatgaaga atcatcaacg 9241 ctatcacttt ctgttcacaa agtatgcgca atccacatcg gtatagaata taatcgggga 9301 tgcctttatc ttgaaaaaat gcacccgcag cttcgctagt aatcagtaaa cgcgggaagt 9361 ggagtcaggc tttttttatg gaagagaaaa tagacaccaa agtagccttc ttctaacctt 9421 aacggaccta cagtgcaaaa agttatcaag agactgcatt atagagcgca caaaggagaa 9481 aaaaagtaat ctaagatgct ttgttagaaa aatagcgctc tcgggatgca tttttgtaga 9541 acaaaaaaga agtatagatt ctttgttggt aaaatagcgc tctcgcgttg catttctgtt 9601 ctgtaaaaat gcagctcaga ttctttgttt gaaaaattag cgctctcgcg ttgcattttt 9661 gttttacaaa aatgaagcac agattcttcg ttggtaaaat agcgctttcg cgttgcattt 9721 ctgttctgta aaaatgcagc tcagattctt tgtttgaaaa attagcgctc tcgcgttgca 9781 tttttgttct acaaaatgaa gcacagatgc ttcgttgctt gcatgcctgc aggtcgagat 9841 ccgggatcga agaaatgatg gtaaatgaaa taggaaatca aggagcatga aggcaaaaga 9901 caaatataag ggtcgaacga aaaataaagt gaaaagtgtt gatatgatgt atttggcttt 9961 gcggcgccga aaaaacgagt ttacgcaatt gcacaatcat gctgactctg tggcggaccc 10021 gcgctcttgc cggcccggcg ataacgctgg gcgtgaggct gtgcccggcg gagttttttg 10081 cgcctgcatt ttccaaggtt taccctgcgc taaggggcga gattggagaa gcaataagaa 10141 tgccggttgg ggttgcgatg atgacgacca cgacaactgg tgtcattatt taagttgccg 10201 aaagaacctg agtgcatttg caacatgagt atactagaag aatgagccaa gacttgcgag 10261 acgcgagttt gccggtggtg cgaacaatag agcgaccatg accttgaagg tgagacgcgc 10321 ataaccgcta gagtactttg aagaggaaac agcaataggg ttgctaccag tataaataga 10381 caggtacata caacactgga aatggttgtc tgtttgagta cgctttcaat tcatttgggt 10441 gtgcacttta ttatgttaca atatggaagg gaactttaca cttctcctat gcacatatat 10501 taattaaagt ccaatgctag tagagaaggg gggtaacacc cctccgcgct cttttccgat 10561 ttttttctaa accgtggaat atttcggat //