LOCUS Exported 5725 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Localization: Microtubules, Excitation: 590, Emission: 649. ACCESSION . VERSION . KEYWORDS mPlum-MAPTau-N-10 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5725) TITLE Davidson Red fluorescent proteins REFERENCE 2 (bases 1 to 5725) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5725 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..304 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 305..508 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 459..479 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 559..568 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1651..2331 /codon_start=1 /product="far-red monomeric derivative of DsRed fluorescent protein (Wang et al., 2004)" /label=mPlum /note="mammalian codon-optimized" /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH STGA" primer_bind 2052..2071 /label=mCherry-F /note="mCherry, forward primer" primer_bind 2246..2269 /label=DsRed1-C /note="DsRed1, forward primer" polyA_signal 2453..2574 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2490..2509) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 2544..2563 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(2581..3036) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2718..2739) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2930..2949 /label=F1ori-R /note="F1 origin, reverse primer" promoter 3063..3167 /gene="bla" /label=AmpR promoter promoter 3169..3526 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(3192..3212) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 3377..3512 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3439..3458 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 3561..4355 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3615..3634) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 4225..4244 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(4543..4562) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 4587..4634 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4963..5551 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5452..5471 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 541 ccggactcag atctcgaggc caccatggct gagccccgcc aggagttcga agtgatggaa 601 gatcacgctg ggacgtacgg gttgggggac aggaaagatc aggggggcta caccatgcac 661 caagaccaag agggtgacac ggacgctggc ctgaaagctg aagaagcagg cattggagac 721 acccccagcc tggaagacga agctgctggt cacgtgaccc aagctcgcat ggtcagtaaa 781 agcaaagacg ggactggaag cgatgacaaa aaagccaagg gggctgatgg taaaacgaag 841 atcgccacac cgcggggagc agcccctcca ggccagaagg gccaggccaa cgccaccagg 901 attccagcaa aaaccccgcc cgctccaaag acaccaccca gctctggtga acctccaaaa 961 tcaggggatc gcagcggcta cagcagcccc ggctccccag gcactcccgg cagccgctcc 1021 cgcaccccgt cccttccaac cccacccacc cgggagccca agaaggtggc agtggtccgt 1081 actccaccca agtcgccgtc ttccgccaag agccgcctgc agacagcccc cgtgcccatg 1141 ccagacctga agaatgtcaa gtccaagatc ggctccactg agaacctgaa gcaccagccg 1201 ggaggcggga aggtgcaaat agtctacaaa ccagttgacc tgagcaaggt gacctccaag 1261 tgtggctcat taggcaacat ccatcataaa ccaggaggtg gccaggtgga agtaaaatct 1321 gagaagcttg acttcaagga cagagtccag tcgaagattg ggtccctgga caatatcacc 1381 cacgtccctg gcggaggaaa taaaaagatt gaaacccaca agctgacctt ccgcgagaac 1441 gccaaagcca agacagacca cggggcggag atcgtgtaca agtcgccagt ggtgtctggg 1501 gacacgtctc cacggcatct cagcaatgtc tcctccaccg gcagcatcga catggtagac 1561 tcgccccagc tcgccacgct agctgacgag gtgtctgcct ccctggccaa gcagggtttg 1621 agctccggag gggatccacc ggtggctacc atggtgagca agggcgagga ggtcatcaag 1681 gagttcatgc gcttcaagga gcacatggag ggctccgtga acggccacga gttcgagatc 1741 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaggct gaaggtgacc 1801 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagatcat gtacggctcc 1861 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag 1921 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag 1981 gactcctccc tgcaggacgg cgagttcatc tacaaggtga aggtgcgcgg caccaacttc 2041 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg 2101 atgtaccccg aggacggcgc cctgaagggc gagatgaaga tgaggctgag gctgaaggac 2161 ggcggccact acgacgccga ggtcaagacc acctacatgg ccaagaagcc cgtgcagctg 2221 cccggcgcct acaagaccga catcaagctg gacatcacct cccacaacga ggactacacc 2281 atcgtggaac agtacgagcg cgccgagggc cgccactcca ccggcgccta agcggccgcg 2341 actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct 2401 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt 2461 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 2521 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaaggcgt 2581 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat 2641 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga 2701 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca 2761 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct 2821 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc 2881 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag 2941 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca 3001 cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg 3061 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga 3121 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtcct gaggcggaaa 3181 gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg 3241 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg 3301 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc 3361 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca 3421 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt 3481 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaagatc gatcaagaga 3541 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg 3601 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg 3661 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt 3721 ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg 3781 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat 3841 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat 3901 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg 3961 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg 4021 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc 4081 tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc 4141 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg 4201 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg 4261 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca 4321 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac 4381 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga 4441 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga 4501 tctcatgctg gagttcttcg cccaccctag ggggaggcta actgaaacac ggaaggagac 4561 aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacggtgt 4621 tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc 4681 accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc 4741 aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc 4801 ctcaggttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat 4861 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt 4921 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 4981 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 5041 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 5101 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 5161 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 5221 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 5281 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 5341 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 5401 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 5461 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 5521 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 5581 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt 5641 ggataaccgt attaccgcca tgcattagtt attaatagta atcaattacg gggtcattag 5701 ttcatagccc atatatggag ttccg //