LOCUS Exported 11513 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Overexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoter. ACCESSION . VERSION . KEYWORDS pJT2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11513) AUTHORS Peng B, Nielsen LK, Kampranis SC, Vickers CE TITLE Engineered protein degradation of farnesyl pyrophosphate synthase is an effective regulatory mechanism to increase monoterpene production in Saccharomyces cerevisiae. JOURNAL Metab Eng. 2018 Feb 19. pii: S1096-7176(17)30214-8. doi: 10.1016/j.ymben.2018.02.005. PUBMED 29471044 REFERENCE 2 (bases 1 to 11513) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..11513 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind complement(771..818) /label=FRT /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." primer_bind 1191..1209 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(1247..1269) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 1369..1388 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" CDS complement(1882..2976) /codon_start=1 /gene="S. cerevisiae LEU2" /product="3-isopropylmalate dehydrogenase, required for leucine biosynthesis" /label=LEU2 /note="yeast auxotrophic marker" /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG DLGGSNSTTEVGDAVAEEVKKILA" promoter complement(2977..3384) /gene="S. cerevisiae LEU2" /label=LEU2 promoter rep_origin complement(3685..4140) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3822..3843) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 4034..4053 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4266..4288 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 4280..4297 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 4281..4297 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 4307..4326 /label=T7 /note="T7 promoter, forward primer" promoter 4307..4325 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" promoter 6626..7033 /gene="S. cerevisiae TEF1" /label=TEF1 promoter /note="promoter for EF-1-alpha" primer_bind complement(9129..9149) /label=T3 /note="T3 promoter, forward primer" promoter complement(9129..9147) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(9168..9184) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(9168..9184) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(9181..9203) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 9192..9208 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(9216..9246) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 9261..9282 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(9399..9416) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(9570..10158) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(9650..9669) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(10329..11189) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 10952..10971 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(11190..11294) /gene="bla" /label=AmpR promoter rep_origin 11321..1150 /label=2u ori /note="yeast 2u plasmid origin of replication" ORIGIN 1 gaatctgagc tgcattttta cagaacagaa atgcaacgcg agagcgctat tttaccaaca 61 aagaatctat acttcttttt tgttctacaa aaatgcatcc cgagagcgct atttttctaa 121 caaagcatct tagattactt tttttctcct ttgtgcgctc tataatgcag tctcttgata 181 actttttgca ctgtaggtcc gttaaggtta gaagaaggct actttggtgt ctattttctc 241 ttccataaaa aaagcctgac tccacttccc gcgtttactg attactagcg aagctgcggg 301 tgcatttttt caagataaag gcatccccga ttatattcta taccgatgtg gattgcgcat 361 actttgtgaa cagaaagtga tagcgttgat gattcttcat tggtcagaaa attatgaacg 421 gtttcttcta ttttgtctct atatactacg tataggaaat gtttacattt tcgtattgtt 481 ttcgattcac tctatgaata gttcttacta caattttttt gtctaaagag taatactaga 541 gataaacata aaaaatgtag aggtcgagtt tagatgcaag ttcaaggagc gaaaggtgga 601 tgggtaggtt atatagggat atagcacaga gatatatagc aaagagatac ttttgagcaa 661 tgtttgtgga agcggtattc gcaatatttt agtagctcgt tacagtccgg tgcgtttttg 721 gttttttgaa agtgcgtctt cagagcgctt ttggttttca aaagcgctct gaagttccta 781 tactttctag agaataggaa cttcggaata ggaacttcaa agcgtttccg aaaacgagcg 841 cttccgaaaa tgcaacgcga gctgcgcaca tacagctcac tgttcacgtc gcacctatat 901 ctgcgtgttg cctgtatata tatatacatg agaagaacgg catagtgcgt gtttatgctt 961 aaatgcgtac ttatatgcgt ctatttatgt aggatgaaag gtagtctagt acctcctgtg 1021 atattatccc attccatgcg gggtatcgta tgcttccttc agcactaccc tttagctgtt 1081 ctatatgctg ccactcctca attggattag tctcatcctt caatgctatc atttcctttg 1141 atattggatc atactaagaa accattatta tcatgacatt aacctataaa aataggcgta 1201 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc 1261 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc 1321 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc 1381 agattgtact gagagtgcac catatcgact acgtcgttaa ggccgtttct gacagagtaa 1441 aattcttgag ggaactttca ccattatggg aaatggttca agaaggtatt gacttaaact 1501 ccatcaaatg gtcaggtcat tgagtgtttt ttatttgttg tatttttttt tttttagaga 1561 aaatcctcca atatataaat taggaatcat agtttcatga ttttctgtta cacctaactt 1621 tttgtgtggt gccctcctcc ttgtcaatat taatgttaaa gtgcaattct ttttccttat 1681 cacgttgagc cattagtatc aatttgctta cctgtattcc tttacatcct cctttttctc 1741 cttcttgata aatgtatgta gattgcgtat atagtttcgt ctaccctatg aacatattcc 1801 attttgtaat ttcgtgtcgt ttctattatg aatttcattt ataaagttta tgtacaaata 1861 tcataaaaaa agagaatctt tttaagcaag gattttctta acttcttcgg cgacagcatc 1921 accgacttcg gtggtactgt tggaaccacc taaatcacca gttctgatac ctgcatccaa 1981 aaccttttta actgcatctt caatggcctt accttcttca ggcaagttca atgacaattt 2041 caacatcatt gcagcagaca agatagtggc gatagggttg accttattct ttggcaaatc 2101 tggagcagaa ccgtggcatg gttcgtacaa accaaatgcg gtgttcttgt ctggcaaaga 2161 ggccaaggac gcagatggca acaaacccaa ggaacctggg ataacggagg cttcatcgga 2221 gatgatatca ccaaacatgt tgctggtgat tataatacca tttaggtggg ttgggttctt 2281 aactaggatc atggcggcag aatcaatcaa ttgatgttga accttcaatg tagggaattc 2341 gttcttgatg gtttcctcca cagtttttct ccataatctt gaagaggcca aaacattagc 2401 tttatccaag gaccaaatag gcaatggtgg ctcatgttgt agggccatga aagcggccat 2461 tcttgtgatt ctttgcactt ctggaacggt gtattgttca ctatcccaag cgacaccatc 2521 accatcgtct tcctttctct taccaaagta aatacctccc actaattctc tgacaacaac 2581 gaagtcagta cctttagcaa attgtggctt gattggagat aagtctaaaa gagagtcgga 2641 tgcaaagtta catggtctta agttggcgta caattgaagt tctttacgga tttttagtaa 2701 accttgttca ggtctaacac taccggtacc ccatttagga ccacccacag cacctaacaa 2761 aacggcatca gccttcttgg aggcttccag cgcctcatct ggaagtggaa cacctgtagc 2821 atcgatagca gcaccaccaa ttaaatgatt ttcgaaatcg aacttgacat tggaacgaac 2881 atcagaaata gctttaagaa ccttaatggc ttcggctgtg atttcttgac caacgtggtc 2941 acctggcaaa acgacgatct tcttaggggc agacattaga atggtatatc cttgaaatat 3001 atatatatat attgctgaaa tgtaaaaggt aagaaaagtt agaaagtaag acgattgcta 3061 accacctatt ggaaaaaaca ataggtcctt aaataatatt gtcaacttca agtattgtga 3121 tgcaagcatt tagtcatgaa cgcttctcta ttctatatga aaagccggtt ccggcgctct 3181 cacctttcct ttttctccca atttttcagt tgaaaaaggt atatgcgtca ggcgacctct 3241 gaaattaaca aaaaatttcc agtcatcgaa tttgattctg tgcgatagcg cccctgtgtg 3301 ttctcgttat gttgaggaaa aaaataatgg ttgctaagag attcgaactc ttgcatctta 3361 cgatacctga gtattcccac agttaactgc ggtcaagata tttcttgaat caggcgcctt 3421 agaccgctcg gccaaacaac caattacttg ttgagaaata gagtataatt atcctataaa 3481 tataacgttt ttgaacacac atgaacaagg aagtacagga caattgattt tgaagagaat 3541 gtggattttg atgtaattgt tgggattcca tttttaataa ggcaataata ttaggtatgt 3601 agatatacta gaagttctcc tcgaccggtc gatatgcggt gtgaaatacc gcacagatgc 3661 gtaaggagaa aataccgcat caggaaattg taagcgttaa tattttgtta aaattcgcgt 3721 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt 3781 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc 3841 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg 3901 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac 3961 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg 4021 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag 4081 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt 4141 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 4201 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 4261 gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat acgactcact 4321 atagggcgaa ttgggtaccg ggccctgcag actttgttat gtagagtttt tttagctacc 4381 tatattccac cataacatca atcatgcggt tgctggtgta tttaccaata atgtttaatg 4441 tatatatata tatatatatn nggggccgta tacttacata tagtagatgt caagcgtagg 4501 cgcttcccct gccggctgtg agggcgccat aaccaaggta tctatagacc gccaatcagc 4561 aaactacctc cgtacattca tgttgcaccc acacatttat acacccagac cgcgacaaat 4621 tacccataag gttgtttgtg acggcgtcgt acaagagaac gtgggaactt tttaggctca 4681 ccaaaaaaga aagaaaaaat acgagttgct gacagaagcc tcaagaaaaa aaaaattctt 4741 cttcgactat gctggaggca gagatgatcg agccggtagt taactatata tagctaaatt 4801 ggttccatca ccttcttttc tggtgtcgct ccttctagtg ctatttctgg cttttcctat 4861 tttttttttt ccatttttct ttctctcttt ctaatatata aattctcttg cattttctat 4921 ttttctctct atctattcta cttgtttatt cccttcaagg ttttttttta aggagtactt 4981 gtttttagaa tatacggtca acgaactata attaactaaa catggcttca gaaaaagaaa 5041 ttaggagaga gagattcttg aacgttttcc ctaaattagt agaggaattg aacgcatcgc 5101 ttttggctta cggtatgcct aaggaagcat gtgactggta tgcccactca ttgaactaca 5161 acactccagg cggtaagcta aatagaggtt tgtccgttgt ggacacgtat gctattctct 5221 ccaacaagac cgttgaacaa ttggggcaag aagaatacga aaaggttgcc attctaggtt 5281 ggtgcattga gttgttgcag gcttacttct tggtcgccga tgatatgatg gacaagtcca 5341 ttaccagaag aggccaacca tgttggtaca aggttcctga agttggggaa attgccatct 5401 gggacgcatt catgttagag gctgctatct acaagctttt gaaatctcac ttcagaaacg 5461 aaaaatacta catagatatc accgaattgt tccatgaggt caccttccaa accgaattgg 5521 gccaattgat ggacttaatc actgcacctg aagacaaagt cgacttgagt aagttctccc 5581 taaagaagca ctccttcata gttactttca agactgctta ctattctttc tacttgcctg 5641 tcgcattggc catgtacgtt gccggtatca cggatgaaaa ggatttgaaa caagccagag 5701 atgtcttgat tccattgggt gaatacttcc aaattcaaga tgactactta gactgcttcg 5761 gtaccccaga acagatcggt aagatcggta cagatatcca agataacaaa tgttcttggg 5821 taatcaacaa ggcattagaa cttgcttccg cagaacaaag aaagacttta gacgaaaatt 5881 acggtaagaa ggactcagtc gcagaagcca aatgcaaaaa gattttcaat gacttgaaaa 5941 ttgaacagct ataccacgaa tatgaagagt ctattgccaa ggatttgaag gccaaaattt 6001 ctcaggtcga tgagtctcgt ggcttcaaag ctgatgtctt aactgcgttc ttgaacaaag 6061 tttacaagag aagcaaataa tagtgagaag ttttgttaga aaataaatca ttttttaatt 6121 gagcattctt attcctattt tatttaaata gttttatgta ttgttagcta catacaacag 6181 tttaaatcaa attttctttt tcccaagtcc aaaatggagg tttattttga tgacccgcat 6241 gcgattatgt tttgaaagta taagactaca tacatgtaca tatatttaaa catgtaaacc 6301 cgtccattat attgcttact ttcttctttt ttgccgtttt gacttggacc tctggtttgc 6361 tatttcctta caatctttgc tacaatggat cccgggttga cagcctagac atcaatagtc 6421 atacaacaga aagcgaccac ccaactttgg ctgataatag cgtataaaca atgcatactt 6481 tgtacgttca aaatacaatg cagtagatat atttatgcat attacatata atacatatca 6541 cataggaagc aacaggcgcg ttggactttt aattttcgag gaccgcgaat ccttacatca 6601 cacccaatcc cccacaagtg atcccccaca caccatagct tcaaaatgtt tctactcctt 6661 ttttactctt ccagattttc tcggactccg cgcatcgccg taccacttca aaacacccaa 6721 gcacagcata ctaaatttcc cctctttctt cctctagggt gtcgttaatt acccgtacta 6781 aaggtttgga aaagaaaaaa gagaccgcct cgtttctttt tcttcgtcga aaaaggcaat 6841 aaaaattttt atcacgtttc tttttcttga aaattttttt ttttgatttt tttctctttc 6901 gatgacctcc cattgatatt taagttaata aacggtcttc aatttctcaa gtttcagttt 6961 catttttctt gttctattac aacttttttt acttcttgct cattagaaag aaagcatagc 7021 aatctaatct aagttttaat tacaaaatgg ctaccgcagc aggtcctatc gcaactaaca 7081 actccccaca aaactccaac gcttacagaa ctccaatcgc tccttccgta ccaattactc 7141 ataaatggtc tatagctgaa gatttgacat gtatttccaa tcctagtaag cacaataacc 7201 ctcaaactgg ttacagatca ttttctgacg aattatacgt taagtacgaa gaaaagttgg 7261 aagatgttag aaaagcatta agagaagttg aagaaaaccc tttggaaggt ttagttatga 7321 tagacgcttt gcaaagattg ggtatcgatt accatttcag aggtgaaatt ggtgcattct 7381 tgcaaaagca acaaatcata tcttcaactc cagatggtta ccctgaacat ggtttgtacg 7441 aagtttcaac attgtttaga ttcttaagac aagaaggtca caatgttacc gctgacgtct 7501 ttaataactt caaggataag gaaggtagat tcagatcaga attgtcaaca gatattagag 7561 gtttgatgtc cttatacgaa gcaagtcaat tgagaataga aggtgaagac atcttagatc 7621 aagctgctga tttctccagt caattgttag gtagatggac aaaagatcct aatcatcacg 7681 aagccagatt ggtttctaac actttaacac atccatacca caagtcattg gctaccttta 7741 tgggtcaaaa attgtcctac atgaactgca agggtccaaa ctgggacggt gtcgataatt 7801 tgcaagaatt agctaagatg gatttgacta tcgtacaaag tatccatcaa aaggaagtat 7861 tccaagtttc tcaatggtgg aaggatacag gtttagccaa tgaattgaaa ttggctagaa 7921 accaaccatt gaagtggtat atgtggccta tggccgcttt aaccgatcca agattcagtg 7981 aagaaagagt tgaattaact aagcctattt cttttatata catcatagat gacatcttcg 8041 acgtctatgg taccattgaa gaattgacct tgtttactga tgcagttaat agatgggaat 8101 tgtctgccgt cgaacaatta ccagactaca tgaaagtatg tttcaaggct ttgtacgatg 8161 ttaccaacga aatcgcatac aaaatctata aaaagcatgg tcaaaaccct attgattcct 8221 tgcaaaagac ttgggctagt ttgtgcaatg catttttagt tgaagcaaag tggttcgcct 8281 ctggtcactt gccaaatgca gaagaatact taaagaacgg tatcatctct tcaggtgttc 8341 atgttgtctt ggcccacatg tttttcttgt taggtgacgg tattacacaa gaatcagttg 8401 atttggtaga tgactatcca ggtatttcca caagtatcgc aaccattttg agattatctg 8461 atgacttggg ttcagccaaa gatgaagacc aagatggtta tgatggttct tacatcgaat 8521 gttacatgaa ggaacataag ggttccagtg tcgattcagc cagagaagaa gtaataagaa 8581 tgatctccga agcatggaaa tgtttgaata aggaatgctt atcaccaaac cctttttctg 8641 aatcattcag aataggttcc ttgaatatgg ctagaatgat ccctatgatg tactcttacg 8701 atgacaacca taacttgcca attttagaag aacacatgaa ggcaatgatc tataacacat 8761 ctttataata gtgagcggat tgagagcaaa tcgttaagtt caggtcaagt aaaaattgat 8821 ttcgaaaact aatttctctt atacaatcct ttgattggac cgtcatcctt tcgaatataa 8881 gattttgtta agaatatttt agacagagat ctactttata tttaatatct agatattaca 8941 taatttcctc tctaataaaa tatcattaat aaaataaaaa tgaagcgatt tgattttgtg 9001 ttgtcaactt agtttgccgc tatgcctctt gggtaatgct attattgaat cgaagggctt 9061 tattatatta ccctttagct tattctgagg tttctgtggc gtccgcggtg gagctccagc 9121 ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt 9181 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag 9241 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg 9301 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg 9361 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc 9421 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 9481 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 9541 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 9601 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 9661 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 9721 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 9781 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 9841 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 9901 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 9961 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt 10021 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 10081 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 10141 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 10201 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 10261 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 10321 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 10381 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 10441 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 10501 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 10561 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 10621 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 10681 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 10741 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 10801 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 10861 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 10921 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 10981 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 11041 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 11101 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 11161 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 11221 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 11281 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg aacgaagcat ctgtgcttca 11341 ttttgtagaa caaaaatgca acgcgagagc gctaattttt caaacaaaga atctgagctg 11401 catttttaca gaacagaaat gcaacgcgaa agcgctattt taccaacgaa gaatctgtgc 11461 ttcatttttg taaaacaaaa atgcaacgcg agagcgctaa tttttcaaac aaa //