LOCUS       Exported                 582 bp ds-DNA     linear   SYN 04-JUL-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pRL-TK let7 F
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Doench JG, Sharp PA
  TITLE     Specificity of microRNA target selection in translational 
            repression.
  JOURNAL   Genes Dev. 2004 Mar 1. 18(5):504-11.
  PUBMED    15014042
REFERENCE   2  (bases 1 to 582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 4, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     polyA_signal    273..394
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(284..303)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     338..357
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 taatactgaa tttgtcaaag taaaaggtct tcatttttcg caagaagatg cacctgatga
       61 aatgggaaaa tatatcaaat cgttcgttga gcgagttctc aaaaatgaac aataattcta
      121 gaaagttttc acaaagctaa caggtacctc gagaactata caacgtctac atcaccggaa
      181 ctatacaacg tctacatcaa ctagtgtacc aagttttcac aaagctaaca ccggatcgcg
      241 ggccctagag cggccgcttc gagcagacat gataagatac attgatgagt ttggacaaac
      301 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
      361 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
      421 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
      481 tggtaaaatc gataaggatc caggtggcac ttttcgggga aatgtgcgcg gaacccctat
      541 ttgtttattt ttctaaatac attcaaatat gtatccgctc at
//
