LOCUS Exported 4317 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION C-terminal AID*-9myc degron cassette with NatR marker for S. cerevisiae. ACCESSION . VERSION . KEYWORDS pNat-AID*-9myc SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4317) AUTHORS Morawska M, Ulrich HD TITLE An expanded tool kit for the auxin-inducible degron system in budding yeast. JOURNAL Yeast. 2013 Sep;30(9):341-51. doi: 10.1002/yea.2967. Epub 2013 Jul 23. PUBMED 23836714 REFERENCE 2 (bases 1 to 4317) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4317 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 232..261 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 268..297 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 304..333 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 352..381 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 388..417 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 424..453 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 472..501 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 508..537 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 544..573 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" promoter 648..991 /label=TEF promoter /note="Ashbya gossypii TEF promoter" CDS 992..1567 /codon_start=1 /gene="Streptomyces noursei nat1" /product="nourseothricin acetyltransferase" /label=NrsR /note="confers resistance to nourseothricin" /translation="MGTTLDDTAYRYRTSVPGEAEAIEALDGSFTTDTVFRVTATGDGF TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVISYSAWNR RLTVEDIEVAPEHRGHGVGRALMGLATEFAGERGAGHLWLEVTNVNAPAIHAYRRMGFT LCGLDTALYDGTASDGERQALYMSMPCP" terminator 1575..1762 /gene="S. cerevisiae ADH1" /label=ADH1 terminator /note="transcription terminator for the S. cerevisiae alcohol dehydrogenase 1 (ADH1) gene" primer_bind complement(1630..1654) /label=pHybLex-R /note="ADH terminator, reverse primer" primer_bind complement(1954..1973) /label=T7 /note="T7 promoter, forward primer" promoter complement(1955..1973) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2060..2077) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(2231..2819) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2311..2330) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(2990..3850) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 3613..3632 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(3851..3955) /gene="bla" /label=AmpR promoter primer_bind 4023..4041 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(4079..4101) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 4201..4220 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" promoter 4301..2 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind 4301..1 /label=SP6 /note="SP6 promoter, forward primer" ORIGIN 1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggtgcagg cgccggggct 61 ggtgctgggg caggtacccc taaagatcca gccaaacctc cggccaaggc acaagttgtg 121 ggatggccac cggtgagatc ataccggaag aacgtgatgg tttcctgcca aaaatcaagc 181 ggtggcccgg aggcggcggc gttcgtgaag gtatcacccg gggctagtgg tgaacaaaag 241 ttgatttctg aagaagattt gaacggtgaa caaaagctaa tctccgagga agacttgaac 301 ggtgaacaaa aattaatctc agaagaagac ttgaacggat cctctagagg tgaacaaaag 361 ttgatttctg aagaagattt gaacggtgaa caaaagctaa tctccgagga agacttgaac 421 ggtgaacaaa aattaatctc agaagaagac ttgaacggat cctctagagg tgaacaaaag 481 ttgatttctg aagaagattt gaacggtgaa caaaagctaa tctccgagga agacttgaac 541 ggtgaacaaa aattaatctc agaagaagac ttgaacggat ccactagcta aaaaggcgcg 601 ccagatctgt ttagcttgcc tcgtccccgc cgggtcaccc ggccagcgac atggaggccc 661 agaataccct ccttgacagt cttgacgtgc gcagctcagg ggcatgatgt gactgtcgcc 721 cgtacattta gcccatacat ccccatgtat aatcatttgc atccatacat tttgatggcc 781 gcacggcgcg aagcaaaaat tacggctcct cgctgcagac ctgcgagcag ggaaacgctc 841 ccctcacaga cgcgttgaat tgtccccacg ccgcgcccct gtagagaaat ataaaaggtt 901 aggatttgcc actgaggttc ttctttcata tacttccttt taaaatcttg ctaggataca 961 gttctcacat cacatccgaa cataaacaac catgggtacc actcttgacg acacggctta 1021 ccggtaccgc accagtgtcc cgggggaagc cgaggccatc gaggcactgg atgggtcctt 1081 caccaccgac accgtcttcc gcgtcaccgc caccggggac ggcttcaccc tgcgggaggt 1141 gccggtggac ccgcccctga ccaaggtgtt ccccgacgac gaatcggacg acgaatcgga 1201 cgacggggag gacggcgacc cggactcccg gacgttcgtc gcgtacgggg acgacggcga 1261 cctggcgggc ttcgtggtca tctcgtactc ggcgtggaac cgccggctga ccgtcgagga 1321 catcgaggtc gccccggagc accgggggca cggggtcggg cgcgcgttga tggggctcgc 1381 gacggagttc gccggcgagc ggggcgccgg gcacctctgg ctggaggtca ccaacgtcaa 1441 cgcaccggcg atccacgcgt accggcggat ggggttcacc ctctgcggcc tggacaccgc 1501 cctgtacgac ggcaccgcct cggacggcga gcggcaggcg ctctacatga gcatgccctg 1561 cccctaatct cgaggcgaat ttcttatgat ttatgatttt tattattaaa taagttataa 1621 aaaaaataag tgtatacaaa ttttaaagtg actcttaggt tttaaaacga aaattcttgt 1681 tcttgagtaa ctctttcctg taggtcaggt tgctttctca ggtatagcat gaggtcgctc 1741 ttattgacca cacctctacc ggcatgccga gcaaatgcct gcaaatcgct ccccatttca 1801 cccaattgta gatatgctaa ctccagcaat gagttgatga atctcggtgt gtattttatg 1861 tcctcagagg acaacacctg ttgtaatcga gctcgaattc atcgatgata tcagatccac 1921 tagtggccta tgcggccgcg gatctgccgg tctccctata gtgagtcgta ttaatttcga 1981 taagccaggt taacctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 2041 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 2101 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 2161 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 2221 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 2281 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 2341 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 2401 cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg 2461 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 2521 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 2581 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 2641 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga 2701 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 2761 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 2821 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 2881 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 2941 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 3001 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 3061 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 3121 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 3181 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 3241 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 3301 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 3361 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 3421 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 3481 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 3541 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 3601 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 3661 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 3721 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 3781 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 3841 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 3901 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 3961 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata 4021 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc 4081 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca 4141 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg 4201 cggcatcaga gcagattgta ctgagagtgc accatatgga catattgtcg ttagaacgcg 4261 gctacaatta atacataacc ttatgtatca tacacatacg atttaggtga cactata //