LOCUS Exported 5882 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expression of secretory immunoglobulin heavy chains in mammalian cells, human IgG3 isotype. ACCESSION . VERSION . KEYWORDS AbVec2.0-IGHG3 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5882) TITLE Immunoglobulin expression vectors JOURNAL Unpublished REFERENCE 2 (bases 1 to 5882) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5882 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 106..127 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 142..172 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 180..196 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 185..207 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 204..220 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 204..220 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" enhancer 249..628 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 629..832 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 783..803 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 829..853 /label=LNCX /note="Human CMV promoter, forward primer" promoter 1063..1081 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind 1063..1080 /label=SP6 /note="SP6 promoter, forward primer" primer_bind 1094..1113 /label=pMT2-F /note="Synthetic intron, forward primer" regulatory 2417..2426 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" polyA_signal 2430..2564 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2467..2486) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 2521..2540 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" promoter 2633..2990 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2656..2676) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 2841..2976 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2903..2922 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(3010..3027) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(3010..3026) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3019..3041) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 3239..3694 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3326..3345) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 3536..3557 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(3711..3730) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 3830..3852 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(3890..3908) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 3976..4080 /gene="bla" /label=AmpR promoter CDS 4081..4941 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(4299..4318) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 5112..5700 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5601..5620 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 5854..5871 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat ccaactggca 61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct 121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat 181 tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga attaattcga 241 gctcgcccga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt 301 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg 361 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc 421 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc 481 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg 541 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat 601 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg 661 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag 721 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt 781 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt 841 gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg 901 ggaccgatcc agcctccgcg gccgggaacg gtgcattgga acgcggattc cccgtgccaa 961 gagtgacgta agtaccgcct atagagtcta taggcccacc cccttggctt cgttagaacg 1021 cggctacaat taatacataa ccttatgtat catacacata cgatttaggt gacactatag 1081 aataacatcc actttgcctt tctctccaca ggtgtccact cccaggtcca actgcacctc 1141 ggttctatcg attgaattcc accatgggat ggtcatgtat catccttttt ctagtagcaa 1201 ctgcaaccgg ttgactaact agctggtcga ccaagggccc aagcgtcttc cccctggcgc 1261 cctgctccag gagcacctct gggggcacag cggccctggg ctgcctggtc aaggactact 1321 tcccagagcc ggtgacggtg tcgtggaact caggcgccct gaccagcggc gtgcacacct 1381 tcccggctgt cctacagtcc tcaggactct actccctcag cagcgtggtg accgtgccct 1441 ccagcagctt gggcacccag acctacacct gcaacgtgaa tcacaagccc agcaacacca 1501 aggtggacaa gagagttgag ctgaaaacac cactgggcga tacaacacac acttgcccaa 1561 gatgccctga accaaaatct tgcgacacac ctccgccttg tccacgatgt cctgagccaa 1621 agagttgcga tacccctcct ccttgtcccc ggtgtcccga acccaagtcc tgtgatacgc 1681 ctccaccatg tcccagatgt ccagcacctg aactcctggg aggaccgtca gtcttcctct 1741 tccccccaaa acccaaggat acccttatga tttcccggac ccctgaggtc acgtgcgtgg 1801 tggtggacgt gagccacgaa gaccccgagg tccagttcaa gtggtacgtg gacggcgtgg 1861 aggtgcataa tgccaagaca aagccgcggg aggagcagta caacagcacg ttccgtgtgg 1921 tcagcgtcct caccgtcctg caccaggact ggctgaacgg caaggagtac aagtgcaagg 1981 tctccaacaa agccctccca gcccccatcg agaaaaccat ctccaaaacc aaaggacagc 2041 cccgagaacc acaggtgtac accctgcccc catcccggga ggagatgacc aagaaccagg 2101 tcagcctgac ctgcctggtc aaaggcttct accccagcga catcgccgtg gagtgggaga 2161 gcagcgggca gccggagaac aactacaaca ccacgcctcc catgctggac tccgacggct 2221 ccttcttcct ctacagcaag ctcaccgtgg acaagagcag gtggcagcag gggaacatct 2281 tctcatgctc cgtgatgcat gaggctctgc acaaccgctt cacgcagaag agcctctccc 2341 tgtctccggg taaatgagtg cgacggccgg caagcccccg ctccccgggc tctcggggtc 2401 gcgcgaggaa agcttggccg ccatggccca acttgtttat tgcagcttat aatggttaca 2461 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt 2521 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcgggaat taattcggcg 2581 cagcaccatg gcctgaaata acctctgaaa gaggaacttg gttaggtacc ttctgaggcg 2641 gaaagaacca tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag 2701 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc 2761 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag 2821 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc 2881 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc 2941 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctgttaa 3001 cagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca 3061 acttaatcgc cttgcagcac atcccccttt cgccagttgg cgtaatagcg aagaggcccg 3121 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta 3181 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac 3241 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct 3301 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg 3361 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt 3421 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca 3481 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga 3541 ctcttgttcc aaactggaac aacactcaac cctatctcgg gctattcttt tgatttataa 3601 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac 3661 gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc 3721 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga 3781 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc 3841 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata 3901 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact 3961 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg 4021 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt 4081 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct 4141 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca 4201 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc 4261 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc 4321 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg 4381 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta 4441 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc 4501 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt 4561 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg 4621 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct 4681 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc 4741 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct 4801 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac 4861 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc 4921 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat 4981 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg 5041 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc 5101 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa 5161 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag 5221 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta 5281 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta 5341 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag 5401 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg 5461 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg 5521 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag 5581 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc 5641 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa 5701 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg 5761 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct 5821 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa 5881 ga //