LOCUS       Exported                 779 bp ds-DNA     linear   SYN 09-JUN-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    VP16-ER beta (short)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Chang C, Norris JD, Gron H, Paige LA, Hamilton PT, Kenan DJ, Fowlkes
            D, McDonnell DP
  TITLE     Dissection of the LXXLL nuclear receptor-coactivator interaction 
            motif using combinatorial peptide libraries: discovery of peptide 
            antagonists of estrogen receptors alpha and beta.
  JOURNAL   Mol Cell Biol. 1999 Dec . 19(12):8226-39.
  PUBMED    10567548
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 9, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      43..52
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             55..75
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             76..309
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
ORIGIN
        1 agtagtgaag aggctttttt ggaggagatc taagctagcg ccgccaccat gggccctaaa
       61 aagaagcgta aagtcgcccc cccgaccgat gtcagcctgg gggacgagct ccacttagac
      121 ggcgaggacg tggcgatggc gcatgccgac gcgctagacg atttcgatct ggacatgttg
      181 ggggacgggg attccccggg tccgggattt accccccacg actccgcccc ctacggcgct
      241 ctggatatgg ccgacttcga gtttgagcag atgtttaccg atgcccttgg aattgacgag
      301 tacggtgggg aattcccggg gatccgtatg aattacagca ttcccagcaa tgtcactaac
      361 ttggaaggtg ggcctggtcg gcagaccaca agcccaaatg tgttgtggcc aacacctggg
      421 cacctttctc ctttagtggt ccatcgccag ttatcacatc tgtatgcgga acctcaaaag
      481 agtccctggt gtgaagcaag atcgctagaa cacaccttac ctgtaaacag agagacactg
      541 aaaaggaagg ttagtgggaa ccgttgcgcc agccctgtta ctggtccagg ttcaaagagg
      601 gatgctcact tctgcgctgt ctgcagcgat tacgcatcgg gatatcacta tggagtctgg
      661 tcgtgtgaag gatgtaaggc cttttttaaa agaagcattc aaggacataa tgattatatt
      721 tgtccagcta caaatcagtg tacaatcgat aaaaaccggc gcaagagctg ccaggcctg
//
