LOCUS Exported 13685 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Cas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:bar. ACCESSION . VERSION . KEYWORDS pAtCas9-dead_3 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 13685) AUTHORS Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF TITLE A multi-purpose toolkit to enable advanced genome engineering in plants. JOURNAL Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. PUBMED 28522548 REFERENCE 2 (bases 1 to 13685) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..13685 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(291..1085) /codon_start=1 /gene="aphA-3" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin" /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF" misc_feature 1510..1534 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" polyA_signal 1612..1786 /label=CaMV poly(A) signal /note="cauliflower mosaic virus polyadenylation signal" CDS complement(1793..2344) /codon_start=1 /gene="Streptomyces hygroscopicus bar" /product="phosphinothricin acetyltransferase" /label=BlpR /note="confers resistance to bialophos or phosphinothricin" /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF WQLDFSLPVPPRPVLPVTEI" promoter complement(2389..3065) /label=CaMV 35S promoter (enhanced) /note="cauliflower mosaic virus 35S promoter with a duplicated enhancer region" protein_bind 3256..3277 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 3292..3322 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 3330..3346 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 3335..3357 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 3354..3370 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 3354..3370 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" promoter 3900..4245 /label=CaMV 35S promoter /note="strong constitutive promoter from cauliflower mosaic virus" CDS 4279..8382 /codon_start=1 /product="catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=dCas9 /note="RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain" /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" CDS 8395..8415 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" primer_bind complement(8696..8713) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(8696..8712) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8705..8727) /label=M13/pUC Forward /note="In lacZ gene" misc_feature 8915..8939 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" CDS 10240..10869 /codon_start=1 /product="stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 StaA /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI" CDS 11298..12371 /codon_start=1 /product="replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 RepA /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI LVMRYRNLIEGEASAGS" rep_origin 12437..12631 /label=pVS1 oriV /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" primer_bind complement(12867..12889) /label=pGEX 3' /note="pGEX vectors, reverse primer" misc_feature 12975..13115 /label=bom /note="basis of mobility region from pBR322" primer_bind 13030..13049 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind complement(13130..13147) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(13301..204) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(13381..13400) /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 61 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 241 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat 301 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca 361 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag 421 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact 481 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc 541 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg 601 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat 661 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga 721 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca 781 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca 841 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg 901 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa 961 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta 1021 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta 1081 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt 1141 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa 1201 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt 1261 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc 1321 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat 1381 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac 1441 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg 1501 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa 1561 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat 1621 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa 1681 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac 1741 cctaattccc ttatctggga actactcaca cattattatg gagaaactcg agtcaaatct 1801 cggtgacggg caggaccgga cggggcggta ccggcaggct gaagtccagc tgccagaaac 1861 ccacgtcatg ccagttcccg tgcttgaagc cggccgcccg cagcatgccg cggggggcat 1921 atccgagcgc ctcgtgcatg cgcacgctcg ggtcgttggg cagcccgatg acagcgacca 1981 cgctcttgaa gccctgtgcc tccagggact tcagcagatg ggtgtagagc gtggagccca 2041 gtcccgtccg ctggtggcgg ggggagacgt acacggtcga ctcggccgtc cagtcgtagg 2101 cgttgcgtgc cttccagggg cccgcgtagg cgatgccggc gacctcgccg tccacctcgg 2161 cgacgagcca gggatagcgc tcccgcagac ggacgaggtc gtccgtccac tcctgcggtt 2221 cctgcggctc ggtacggaag ttgaccgtgc ttgtctcgat gtagtggttg acgatggtgc 2281 agaccgccgg catgtccgcc tcggtggcac ggcggatgtc ggccgggcgt cgttctgggc 2341 tcatggtaga ctcgagagag atagatttgt agagagagac tggtgatttc agcgtgtcct 2401 ctccaaatga aatgaacttc cttatataga ggaagggtct tgcgaaggat agtgggattg 2461 tgcgtcatcc cttacgtcag tggagatatc acatcaatcc acttgctttg aagacgtggt 2521 tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact 2581 gtcggcagag gcatcttgaa cgatagcctt tcctttatcg caatgatggc atttgtaggt 2641 gccaccttcc ttttctactg tccttttgat gaagtgacag atagctgggc aatggaatcc 2701 gaggaggttt cccgatatta ccctttgttg aaaagtctca atagcccttt ggtcttctga 2761 gactgtatct ttgatattct tggagtagac gagagtgtcg tgctccacca tgttcacatc 2821 aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg 2881 gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttgaacgata gcctttcctt 2941 tatcgcaatg atggcatttg taggtgccac cttccttttc tactgtcctt ttgatgaagt 3001 gacagatagc tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag 3061 tctcaatagc cctttggtct tctgagactg tatctttgat attcttggag tagacgagag 3121 tgtcgtgctc caccatgttg gcaagctgct ctagccaata cgcaaaccgc ctctccccgc 3181 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag 3241 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt 3301 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa 3361 cagctatgac atgattacga attcgagctc ggtacccggg gatcggcgcg ccagatttgc 3421 cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta cgcagcaggt 3481 atcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct tcccaagaag 3541 gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga gaaagatata 3601 tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct tcacaaacca 3661 aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc aaaggccatg 3721 gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg cgaacagttc 3781 atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat ggtggagcac 3841 gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca aagggcaatt 3901 gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg cccagctatc 3961 tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg ccatcattgc 4021 gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa agatggaccc 4081 ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg 4141 gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta tccttcgcaa 4201 gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg gactcctgca 4261 ggtagatcgc tcgtcgacat ggataagaag tactctatcg gactcgctat cggaactaac 4321 tctgtgggat gggctgtgat caccgatgag tacaaggtgc catctaagaa gttcaaggtt 4381 ctcggaaaca ccgataggca ctctatcaag aaaaacctta tcggtgctct cctcttcgat 4441 tctggtgaaa ctgctgaggc taccagactc aagagaaccg ctagaagaag gtacaccaga 4501 agaaagaaca ggatctgcta cctccaagag atcttctcta acgagatggc taaagtggat 4561 gattcattct tccacaggct cgaagagtca ttcctcgtgg aagaagataa gaagcacgag 4621 aggcacccta tcttcggaaa catcgttgat gaggtggcat accacgagaa gtaccctact 4681 atctaccacc tcagaaagaa gctcgttgat tctactgata aggctgatct caggctcatc 4741 tacctcgctc tcgctcacat gatcaagttc agaggacact tcctcatcga gggtgatctc 4801 aaccctgata actctgatgt ggataagttg ttcatccagc tcgtgcagac ctacaaccag 4861 cttttcgaag agaaccctat caacgcttca ggtgtggatg ctaaggctat cctctctgct 4921 aggctctcta agtcaagaag gcttgagaac ctcattgctc agctccctgg tgagaagaag 4981 aacggacttt tcggaaactt gatcgctctc tctctcggac tcacccctaa cttcaagtct 5041 aacttcgatc tcgctgagga tgcaaagctc cagctctcaa aggataccta cgatgatgat 5101 ctcgataacc tcctcgctca gatcggagat cagtacgctg atttgttcct cgctgctaag 5161 aacctctctg atgctatcct cctcagtgat atcctcagag tgaacaccga gatcaccaag 5221 gctccactct cagcttctat gatcaagaga tacgatgagc accaccagga tctcacactt 5281 ctcaaggctc ttgttagaca gcagctccca gagaagtaca aagagatttt cttcgatcag 5341 tctaagaacg gatacgctgg ttacatcgat ggtggtgcat ctcaagaaga gttctacaag 5401 ttcatcaagc ctatcctcga gaagatggat ggaaccgagg aactcctcgt gaagctcaat 5461 agagaggatc ttctcagaaa gcagaggacc ttcgataacg gatctatccc tcatcagatc 5521 cacctcggag agttgcacgc tatccttaga aggcaagagg atttctaccc attcctcaag 5581 gataacaggg aaaagattga gaagattctc accttcagaa tcccttacta cgtgggacct 5641 ctcgctagag gaaactcaag attcgcttgg atgaccagaa agtctgagga aaccatcacc 5701 ccttggaact tcgaagaggt ggtggataag ggtgctagtg ctcagtcttt catcgagagg 5761 atgaccaact tcgataagaa ccttccaaac gagaaggtgc tccctaagca ctctttgctc 5821 tacgagtact tcaccgtgta caacgagttg accaaggtta agtacgtgac cgagggaatg 5881 aggaagcctg cttttttgtc aggtgagcaa aagaaggcta tcgttgatct cttgttcaag 5941 accaacagaa aggtgaccgt gaagcagctc aaagaggatt acttcaagaa aatcgagtgc 6001 ttcgattcag ttgagatttc tggtgttgag gataggttca acgcatctct cggaacctac 6061 cacgatctcc tcaagatcat taaggataag gatttcttgg ataacgagga aaacgaggat 6121 atcttggagg atatcgttct taccctcacc ctctttgaag atagagagat gattgaagaa 6181 aggctcaaga cctacgctca tctcttcgat gataaggtga tgaagcagtt gaagagaaga 6241 agatacactg gttggggaag gctctcaaga aagctcatta acggaatcag ggataagcag 6301 tctggaaaga caatccttga tttcctcaag tctgatggat tcgctaacag aaacttcatg 6361 cagctcatcc acgatgattc tctcaccttt aaagaggata tccagaaggc tcaggtttca 6421 ggacagggtg atagtctcca tgagcatatc gctaacctcg ctggatctcc tgcaatcaag 6481 aagggaatcc tccagactgt gaaggttgtg gatgagttgg tgaaggtgat gggaaggcat 6541 aagcctgaga acatcgtgat cgaaatggct agagagaacc agaccactca gaagggacag 6601 aagaactcta gggaaaggat gaagaggatc gaggaaggta tcaaagagct tggatctcag 6661 atcctcaaag agcaccctgt tgagaacact cagctccaga atgagaagct ctacctctac 6721 tacctccaga acggaaggga tatgtatgtg gatcaagagt tggatatcaa caggctctct 6781 gattacgatg ttgatgctat cgtgccacag tcattcttga aggatgattc tatcgataac 6841 aaggtgctca ccaggtctga taagaacagg ggtaagagtg ataacgtgcc aagtgaagag 6901 gttgtgaaga aaatgaagaa ctattggagg cagctcctca acgctaagct catcactcag 6961 agaaagttcg ataacttgac taaggctgag aggggaggac tctctgaatt ggataaggca 7021 ggattcatca agaggcagct tgtggaaacc aggcagatca ctaagcacgt tgcacagatc 7081 ctcgattcta ggatgaacac caagtacgat gagaacgata agttgatcag ggaagtgaag 7141 gttatcaccc tcaagtcaaa gctcgtgtct gatttcagaa aggatttcca attctacaag 7201 gtgagggaaa tcaacaacta ccaccacgct cacgatgctt accttaacgc tgttgttgga 7261 accgctctca tcaagaagta tcctaagctc gagtcagagt tcgtgtacgg tgattacaag 7321 gtgtacgatg tgaggaagat gatcgctaag tctgagcaag agatcggaaa ggctaccgct 7381 aagtatttct tctactctaa catcatgaat ttcttcaaga ccgagattac cctcgctaac 7441 ggtgagatca gaaagaggcc actcatcgag acaaacggtg aaacaggtga gatcgtgtgg 7501 gataagggaa gggatttcgc taccgttaga aaggtgctct ctatgccaca ggtgaacatc 7561 gttaagaaaa ccgaggtgca gaccggtgga ttctctaaag agtctatcct ccctaagagg 7621 aactctgata agctcattgc taggaagaag gattgggacc ctaagaaata cggtggtttc 7681 gattctccta ccgtggctta ctctgttctc gttgtggcta aggttgagaa gggaaagagt 7741 aagaagctca agtctgttaa ggaacttctc ggaatcacta tcatggaaag gtcatctttc 7801 gagaagaacc caatcgattt cctcgaggct aagggataca aagaggttaa gaaggatctc 7861 atcatcaagc tcccaaagta ctcactcttc gaactcgaga acggtagaaa gaggatgctc 7921 gcttctgctg gtgagcttca aaagggaaac gagcttgctc tcccatctaa gtacgttaac 7981 tttctttacc tcgcttctca ctacgagaag ttgaagggat ctccagaaga taacgagcag 8041 aagcaacttt tcgttgagca gcacaagcac tacttggatg agatcatcga gcagatctct 8101 gagttctcta aaagggtgat cctcgctgat gcaaacctcg ataaggtgtt gtctgcttac 8161 aacaagcaca gagataagcc tatcagggaa caggcagaga acatcatcca tctcttcacc 8221 cttaccaacc tcggtgctcc tgctgctttc aagtacttcg atacaaccat cgataggaag 8281 agatacacct ctaccaaaga agtgctcgat gctaccctca tccatcagtc tatcactgga 8341 ctctacgaga ctaggatcga tctctcacag ctcggtggtg attcaagggc tgatcctaag 8401 aagaagagga aggtttgact cgagatatga agatgaagat gaaatatttg gtgtgtcaaa 8461 taaaaagctt gtgtgcttaa gtttgtgttt ttttcttggc ttgttgtgtt atgaatttgt 8521 ggctttttct aatattaaat gaatgtaaga tcacattata atgaataaac aaatgtttct 8581 ataatccatt gtgaatgttt tgttggatct cttctgcagc atataactac tgtatgtgct 8641 atggtatgga ctatggaata tgattaaaga taaggagctc cggtgacgga ctggcactgg 8701 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg 8761 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt 8821 cccaacagtt gcgcagcctg aatggcgaat gctagagcag cttgagcttg gatcagattg 8881 tcgtttcccg ccttcagttt aaactatcag tgtttgacag gatatattgg cgggtaaacc 8941 taagagaaaa gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat 9001 ccgttcgtcc atttgtatgt gcatgccaac cacagggttc ccctcgggat caaagtactt 9061 tgatccaacc cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtctt 9121 ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt 9181 cctggcgttt tcttgtcgcg tgttttagtc gcataaagta gaatacttgc gactagaacc 9241 ggagacatta cgccatgaac aagagcgccg ccgctggcct gctgggctat gcccgcgtca 9301 gcaccgacga ccaggacttg accaaccaac gggccgaact gcacgcggcc ggctgcacca 9361 agctgttttc cgagaagatc accggcacca ggcgcgaccg cccggagctg gccaggatgc 9421 ttgaccacct acgccctggc gacgttgtga cagtgaccag gctagaccgc ctggcccgca 9481 gcacccgcga cctactggac attgccgagc gcatccagga ggccggcgcg ggcctgcgta 9541 gcctggcaga gccgtgggcc gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt 9601 tcgccggcat tgccgagttc gagcgttccc taatcatcga ccgcacccgg agcgggcgcg 9661 aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc taccctcacc ccggcacaga 9721 tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag gcggctgcac 9781 tgcttggcgt gcatcgctcg accctgtacc gcgcacttga gcgcagcgag gaagtgacgc 9841 ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc attgaccgag gccgacgccc 9901 tggcggccgc cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc aggacggcca 9961 ggacgaaccg tttttcatta ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt 10021 gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc 10081 tgatgccaag ctggcggcct ggccggccag cttggccgct gaagaaaccg agcgccgccg 10141 tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat 10201 gatgcgatga gtaaataaac aaatacgcaa ggggaacgca tgaaggttat cgctgtactt 10261 aaccagaaag gcgggtcagg caagacgacc atcgcaaccc atctagcccg cgccctgcaa 10321 ctcgccgggg ccgatgttct gttagtcgat tccgatcccc agggcagtgc ccgcgattgg 10381 gcggccgtgc gggaagatca accgctaacc gttgtcggca tcgaccgccc gacgattgac 10441 cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg 10501 gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc ggtgcagcca 10561 agcccttacg acatatgggc caccgccgac ctggtggagc tggttaagca gcgcattgag 10621 gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc 10681 atcggcggtg aggttgccga ggcgctggcc gggtacgagc tgcccattct tgagtcccgt 10741 atcacgcagc gcgtgagcta cccaggcact gccgccgccg gcacaaccgt tcttgaatca 10801 gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa 10861 ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg 10921 gccgtccgag cgcacgcagc agcaaggctg caacgttggc cagcctggca gacacgccag 10981 ccatgaagcg ggtcaacttt cagttgccgg cggaggatca caccaagctg aagatgtacg 11041 cggtacgcca aggcaagacc attaccgagc tgctatctga atacatcgcg cagctaccag 11101 agtaaatgag caaatgaata aatgagtaga tgaattttag cggctaaagg aggcggcatg 11161 gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc 11221 ggttggccag gcgtaagcgg ctgggttgcc tgccggccct gcaatggcac tggaaccccc 11281 aagcccgagg aatcggcgtg agcggtcgca aaccatccgg cccggtacaa atcggcgcgg 11341 cgctgggtga tgacctggtg gagaagttga aggccgcgca ggccgcccag cggcaacgca 11401 tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag 11461 aatcccggca accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg 11521 agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca 11581 tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc 11641 gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc atggccagtg 11701 tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat 11761 accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac 11821 tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca 11881 ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc 11941 tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa 12001 ccgggcggcc ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag 12061 aaggcaagaa cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca 12121 tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt 12181 tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca 12241 ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg 12301 ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg 12361 ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc 12421 gaaaaggtct ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga 12481 accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag 12541 tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta 12601 aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gcccaagagc 12661 tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc 12721 ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc 12781 gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc accctgcctc 12841 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca 12901 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt 12961 ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc 13021 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac 13081 cgcacagatg cgtaaggaga aaataccgca tcaggccctc ttccgcttcc tcgctcactg 13141 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 13201 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 13261 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 13321 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 13381 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 13441 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 13501 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 13561 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 13621 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 13681 ggtat //