LOCUS Exported 16383 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Cas9 only expression, Cas9 Type: TaCas9_D10A+H840A (dead), Plant Selection: PvUbi2:hpt II. ACCESSION . VERSION . KEYWORDS pTaCas9-dead_5 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 16383) AUTHORS Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF TITLE A multi-purpose toolkit to enable advanced genome engineering in plants. JOURNAL Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922. PUBMED 28522548 REFERENCE 2 (bases 1 to 16383) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..16383 /organism="synthetic DNA construct" /mol_type="other DNA" CDS complement(291..1085) /codon_start=1 /gene="aphA-3" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin" /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF" misc_feature 1510..1534 /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" polyA_signal 1612..1786 /label=CaMV poly(A) signal /note="cauliflower mosaic virus polyadenylation signal" CDS complement(1827..2852) /codon_start=1 /gene="aph(4)-Ia" /product="aminoglycoside phosphotransferase from E. coli" /label=HygR /note="confers resistance to hygromycin" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK K" protein_bind 4792..4813 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 4828..4858 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 4866..4882 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 4871..4893 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 4890..4906 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 4890..4906 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" CDS 6962..11065 /codon_start=1 /product="catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=dCas9 /note="RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain" /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" CDS 11066..11113 /codon_start=1 /product="bipartite nuclear localization signal from nucleoplasmin" /label=nucleoplasmin NLS /translation="KRPAATKKAGQAKKKK" primer_bind complement(11394..11411) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(11394..11410) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(11403..11425) /label=M13/pUC Forward /note="In lacZ gene" misc_feature 11613..11637 /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" CDS 12938..13567 /codon_start=1 /product="stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 StaA /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI" CDS 13996..15069 /codon_start=1 /product="replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" /label=pVS1 RepA /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI LVMRYRNLIEGEASAGS" rep_origin 15135..15329 /label=pVS1 oriV /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" primer_bind complement(15565..15587) /label=pGEX 3' /note="pGEX vectors, reverse primer" misc_feature 15673..15813 /label=bom /note="basis of mobility region from pBR322" primer_bind 15728..15747 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind complement(15828..15845) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(15999..204) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(16079..16098) /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 61 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 241 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat 301 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca 361 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag 421 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact 481 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc 541 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg 601 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat 661 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga 721 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca 781 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca 841 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg 901 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa 961 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta 1021 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta 1081 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt 1141 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa 1201 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt 1261 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc 1321 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat 1381 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac 1441 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg 1501 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa 1561 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat 1621 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa 1681 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac 1741 cctaattccc ttatctggga actactcaca cattattatg gagaaactcg agcttgtcga 1801 tcgacagatc ccggtcggca tctactctat ttctttgccc tcggacgagt gctggggcgt 1861 cggtttccac tatcggcgag tacttctaca cagccatcgg tccagacggc cgcgcttctg 1921 cgggcgattt gtgtacgccc gacagtcccg gctccggatc ggacgattgc gtcgcatcga 1981 ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca agctctgata gagttggtca 2041 agaccaatgc ggagcatata cgcccggagt cgtggcgatc ctgcaagctc cggatgcctc 2101 cgctcgaagt agcgcgtctg ctgctccata caagccaacc acggcctcca gaagaagatg 2161 ttggcgacct cgtattggga atccccgaac atcgcctcgc tccagtcaat gaccgctgtt 2221 atgcggccat tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg 2281 acttcggggc agtcctcggc ccaaagcatc agctcatcga gagcctgcgc gacggacgca 2341 ctgacggtgt cgtccatcac agtttgccag tgatacacat ggggatcagc aatcgcgcat 2401 atgaaatcac gccatgtagt gtattgaccg attccttgcg gtccgaatgg gccgaacccg 2461 ctcgtctggc taagatcggc cgcagcgatc gcatccatag cctccgcgac cggttgtaga 2521 acagcgggca gttcggtttc aggcaggtct tgcaacgtga caccctgtga acggcgggag 2581 atgcaatagg tcaggctctc gctaaactcc ccaatgtcaa gcacttccgg aatcgggagc 2641 gcggccgatg caaagtgccg ataaacataa cgatctttgt agaaaccatc ggcgcagcta 2701 tttacccgca ggacatatcc acgccctcct acatcgaagc tgaaagcacg agattcttcg 2761 ccctccgaga gctgcatcag gtcggacacg ctgtcgaact tttcgatcag aaacttctcg 2821 acagacgtcg cggtgagttc aggctttttc ataagcttct gcaaaagaga accagacaac 2881 agggtaagtg cctagcagta aacaaacaga actcatcaca agcaaacagc aacatcatat 2941 tcataccaac aggtcatgtg tgttcatcac atcattagta ctaagcatgc catcatccaa 3001 gtatatcaaa gtaagggcaa agagcatgca tgatcatcag gtgcacaaaa gaatcatcaa 3061 attgtagcag tacatatctt catctatcat gcatatctat ccataacagg acgatgcatg 3121 ttgaccaggt aaaagctaca ggatcctata ggaacagcag cgtatatatc ttcaccaatc 3181 ttagcatctt aatcatgtgg cacatgcagt ttcaatttaa gcacatgagc tagttgatta 3241 tgaggtacca gagaatcatc aaactgatgt agcagcatat atcctcatct atcatgcaat 3301 ctaatctaat ctaactaaac aggaaaggtg tgctattcag ttaaaagcta ccgcatcata 3361 tacaaacggc agcataagaa aaagcataat catcttaatc atgcaacaaa cgcagattca 3421 taataagccc aagagctagc ttgtgatgat cttattctac tctgatctac agcaatcaga 3481 taacgaccta accttgcaca tggcaacaaa acaatcgatc ggacgaatca gttgtttgtt 3541 cctagctagc accatcgaac cagataatag atgcacgtac agatcccgaa aacgaaccca 3601 aaaacagggc agacctagct gaacctaggc agcgacccag cagatcgtga gaacgatctc 3661 atctacgaac agcctagaag caaccccacg attcccggac aaacgaccta aaatccccac 3721 aaatcacatg agcatgacag gataaacagc ggaaccgatc agatctacac gaaaacccca 3781 cctcccagcc acccacgatc aggaaacacg cggatctagc atgatttcgt caacgcctca 3841 gcctagttcc tagccacaga ccaagcagaa ccaccaaacc acgccgagcg aggagatggg 3901 gcaagaggac gggggagacg atcgccgtac cttgaagcgg gggaaggatc gccgagggtc 3961 gcgaggagag caatttggat ttggaacccg ggggttgtgc gctccgaacg atgagacgat 4021 gtgagattgt gggaagaggc gcggagggcc ctgtatttat gggctgcgac ggggggagga 4081 gaggtgggga gggttgggga aggaatcccc cacccgtgcc gtgacggttc cgggccgtgt 4141 gagaggagcc cgctcgtctc cgccacgcaa tttccgcgat cggagcggag ctttcgagag 4201 gcggctggat ggttggtggc cgttagattt gtagacgccg ttaacgcctc gcctccaccg 4261 ggaagagttt tgagcagccg cttatgacaa tggcttaacg acgttagacg gagcgttagt 4321 ggcaggccat gatagaatag acgtattgca atgggattat aattaaataa ataagaatat 4381 aataagatat ggcaagtcgg cactcatgac atggtcttcg aaatgatagt gctcactttc 4441 ttagccgaga aagttgacgc gactgattta gaagttaaga tattatttct ctcttctttt 4501 ctttcctcgt catataagga tgaaataaac tttagagatt gccggtagtg attttggatt 4561 tcggcgatca ggcttggttt gccggtttcg gacggtgtgc cttaggccac ccgcagtgta 4621 tcttgtaatg ttcaaccgat aagcaagggt ggggctcaag caagtagtaa acaactatgt 4681 caaatgtcac catggttatg gtcttgttta gttggcttct tggccgattc attaatgcag 4741 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag 4801 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg 4861 tggaattgtg agcggataac aatttcacac aggaaacagc tatgacatga ttacgaattc 4921 gagctcggta cccggggatc tgcaggcgcg cctgcagtgc agcgtgaccc ggtcgtgccc 4981 ctctctagag ataatgagca ttgcatgtct aagttataaa aaattaccac atattttttt 5041 tgtcacactt gtttgaagtg cagtttatct atctttatac atatatttaa actttactct 5101 acgaataata taatctatag tactacaata atatcagtgt tttagagaat catataaatg 5161 aacagttaga catggtctaa aggacaattg agtattttga caacaggact ctacagtttt 5221 atctttttag tgtgcatgtg ttctcctttt tttttgcaaa tagcttcacc tatataatac 5281 ttcatccatt ttattagtac atccatttag ggtttagggt taatggtttt tatagactaa 5341 tttttttagt acatctattt tattctattt tagcctctaa attaagaaaa ctaaaactct 5401 attttagttt ttttatttaa taatttagat ataaaataga ataaaataaa gtgactaaaa 5461 attaaacaaa taccctttaa gaaattaaaa aaactaagga aacatttttc ttgtttcgag 5521 tagataatgc cagcctgtta aacgccgtcg acgagtctaa cggacaccaa ccagcgaacc 5581 agcagcgtcg cgtcgggcca agcgaagcag acggcacggc atctctgtcg ctgcctctgg 5641 acccctctcg agagttccgc tccaccgttg gacttgctcc gctgtcggca tccagaaatt 5701 gcgtggcgga gcggcagacg tgagccggca cggcaggcgg cctcctcctc ctctcacggc 5761 accggcagct acgggggatt cctttcccac cgctccttcg ctttcccttc ctcgcccgcc 5821 gtaataaata gacaccccct ccacaccctc tttccccaac ctcgtgttgt tcggagcgca 5881 cacacacaca accagatctc ccccaaatcc acccgtcggc acctccgctt caaggtacgc 5941 cgctcgtcct cccccccccc ctctctacct tctctagatc ggcgttccgg tccatggtta 6001 gggcccggta gttctacttc tgttcatgtt tgtgttagat ccgtgtttgt gttagatccg 6061 tgctgctagc gttcgtacac ggatgcgacc tgtacgtcag acacgttctg attgctaact 6121 tgccagtgtt tctctttggg gaatcctggg atggctctag ccgttccgca gacgggatcg 6181 atttcatgat tttttttgtt tcgttgcata gggtttggtt tgcccttttc ctttatttca 6241 atatatgccg tgcacttgtt tgtcgggtca tcttttcatg cttttttttg tcttggttgt 6301 gatgatgtgg tctggttggg cggtcgttct agatcggagt agaattaatt ctgtttcaaa 6361 ctacctggtg gatttattaa ttttggatct gtatgtgtgt gccatacata ttcatagtta 6421 cgaattgaag atgatggatg gaaatatcga tctaggatag gtatacatgt tgatgcgggt 6481 tttactgatg catatacaga gatgcttttt gttcgcttgg ttgtgatgat gtggtgtggt 6541 tgggcggtcg ttcattcgtt ctagatcgga gtagaatact gtttcaaact acctggtgta 6601 tttattaatt ttggaactgt atgtgtgtgt catacatctt catagttacg agtttaagat 6661 ggatggaaat atcgatctag gataggtata catgttgatg tgggttttac tgatgcatat 6721 acatgatggc atatgcagca tctattcata tgctctaacc ttgagtacct atctattata 6781 ataaacaagt atgttttata attattttga tcttgatata cttggatgat ggcatatgca 6841 gcagctatat gtggattttt ttagccctgc cttcatacgc tatttatttg cttggtactg 6901 tttcttttgt cgatgctcac cctgttgttt ggtgttactt cctgcagggc gatctattcg 6961 aatggacaag aagtactcga tcggcctcgc catcgggacg aactcagttg gctgggccgt 7021 gatcaccgac gagtacaagg tgccctctaa gaagttcaag gtcctgggga acaccgaccg 7081 ccattccatc aagaagaacc tcatcggcgc tctcctgttc gacagcgggg agaccgctga 7141 ggctacgagg ctcaagagaa ccgctaggcg ccggtacacg agaaggaaga acaggatctg 7201 ctacctccaa gagattttct ccaacgagat ggccaaggtt gacgattcat tcttccaccg 7261 cctggaggag tctttcctcg tggaggagga taagaagcac gagcggcatc ccatcttcgg 7321 caacatcgtg gacgaggttg cctaccacga gaagtaccct acgatctacc atctgcggaa 7381 gaagctcgtg gactccaccg ataaggcgga cctcagactg atctacctcg ctctggccca 7441 catgatcaag ttccgcggcc atttcctgat cgagggggat ctcaacccag acaacagcga 7501 tgttgacaag ctgttcatcc aactcgtgca gacctacaac caactcttcg aggagaaccc 7561 gatcaacgcc tctggcgtgg acgcgaaggc tatcctgtcc gcgaggctct cgaagtccag 7621 gaggctggag aacctgatcg ctcagctccc aggcgagaag aagaacggcc tgttcgggaa 7681 cctcatcgct ctcagcctgg ggctcacccc gaacttcaag tcgaacttcg atctcgctga 7741 ggacgccaag ctgcaactct ccaaggacac ctacgacgat gacctcgata acctcctggc 7801 ccagatcggc gatcaatacg cggacctgtt cctcgctgcc aagaacctgt cggacgccat 7861 cctcctgtca gatatcctcc gcgtgaacac cgagatcacg aaggctccac tctctgcctc 7921 catgatcaag cgctacgacg agcaccatca ggatctgacc ctcctgaagg cgctggtccg 7981 ccaacagctc ccggagaagt acaaggagat tttcttcgat cagtcgaaga acggctacgc 8041 tgggtacatc gacggcgggg cctcacaaga ggagttctac aagttcatca agccaatcct 8101 ggagaagatg gacggcacgg aggagctcct ggtgaagctc aacagggagg acctcctgcg 8161 gaagcagaga accttcgata acggcagcat cccccaccaa atccatctcg gggagctgca 8221 cgccatcctg agaaggcaag aggacttcta ccctttcctc aaggataacc gggagaagat 8281 cgagaagatc ctgaccttca gaatcccata ctacgtcggc cctctcgcgc gggggaactc 8341 aagattcgct tggatgaccc gcaagtctga ggagaccatc acgccgtgga acttcgagga 8401 ggtggtggac aagggcgcta gcgctcagtc gttcatcgag aggatgacca acttcgacaa 8461 gaacctgccc aacgagaagg tgctccctaa gcactcgctc ctgtacgagt acttcaccgt 8521 ctacaacgag ctcacgaagg tgaagtacgt caccgagggc atgcgcaagc cagcgttcct 8581 gtccggggag cagaagaagg ctatcgtgga cctcctgttc aagaccaacc ggaaggtcac 8641 ggttaagcaa ctcaaggagg actacttcaa gaagatcgag tgcttcgatt cggtcgagat 8701 cagcggcgtt gaggaccgct tcaacgccag cctcgggacc taccacgatc tcctgaagat 8761 catcaaggat aaggacttcc tggacaacga ggagaacgag gatatcctgg aggacatcgt 8821 gctgaccctc acgctgttcg aggacaggga gatgatcgag gagcgcctga agacgtacgc 8881 ccatctcttc gatgacaagg tcatgaagca actcaagcgc cggagataca ccggctgggg 8941 gaggctgtcc cgcaagctca tcaacggcat ccgggacaag cagtccggga agaccatcct 9001 cgacttcctg aagagcgatg gcttcgccaa caggaacttc atgcaactga tccacgatga 9061 cagcctcacc ttcaaggagg atatccaaaa ggctcaagtg agcggccagg gggactcgct 9121 gcacgagcat atcgcgaacc tcgctggctc ccccgcgatc aagaagggca tcctccagac 9181 cgtgaaggtt gtggacgagc tcgtgaaggt catgggccgg cacaagcctg agaacatcgt 9241 catcgagatg gccagagaga accaaaccac gcagaagggg caaaagaact ctagggagcg 9301 catgaagcgc atcgaggagg gcatcaagga gctggggtcc caaatcctca aggagcaccc 9361 agtggagaac acccaactgc agaacgagaa gctctacctg tactacctcc agaacggcag 9421 ggatatgtac gtggaccaag agctggatat caaccgcctc agcgattacg acgtcgatgc 9481 tatcgttccc cagtctttcc tgaaggatga ctccatcgac aacaaggtcc tcaccaggtc 9541 ggacaagaac cgcggcaagt cagataacgt tccatctgag gaggtcgtta agaagatgaa 9601 gaactactgg aggcagctcc tgaacgccaa gctgatcacg caaaggaagt tcgacaacct 9661 caccaaggct gagagaggcg ggctctcaga gctggacaag gccggcttca tcaagcggca 9721 gctggtcgag accagacaaa tcacgaagca cgttgcgcaa atcctcgact ctcggatgaa 9781 cacgaagtac gatgagaacg acaagctgat cagggaggtt aaggtgatca ccctgaagtc 9841 taagctcgtc tccgacttca ggaaggattt ccagttctac aaggttcgcg agatcaacaa 9901 ctaccaccat gcccatgacg cttacctcaa cgctgtggtc ggcaccgctc tgatcaagaa 9961 gtacccaaag ctggagtccg agttcgtgta cggggactac aaggtttacg atgtgcgcaa 10021 gatgatcgcc aagtcggagc aagagatcgg caaggctacc gccaagtact tcttctactc 10081 aaacatcatg aacttcttca agaccgagat cacgctggcc aacggcgaga tccggaagag 10141 accgctcatc gagaccaacg gcgagacggg ggagatcgtg tgggacaagg gcagggattt 10201 cgcgaccgtc cgcaaggttc tctccatgcc ccaggtgaac atcgtcaaga agaccgaggt 10261 ccaaacgggc gggttctcaa aggagtctat cctgcctaag cggaacagcg acaagctcat 10321 cgccagaaag aaggactggg acccaaagaa gtacggcggg ttcgacagcc ctaccgtggc 10381 ctactcggtc ctggttgtgg cgaaggttga gaagggcaag tccaagaagc tcaagagcgt 10441 gaaggagctc ctggggatca ccatcatgga gaggtccagc ttcgagaaga acccaatcga 10501 cttcctggag gccaagggct acaaggaggt gaagaaggac ctgatcatca agctcccgaa 10561 gtactctctc ttcgagctgg agaacggcag gaagagaatg ctggcttccg ctggcgagct 10621 ccagaagggg aacgagctcg cgctgccaag caagtacgtg aacttcctct acctggcttc 10681 ccactacgag aagctcaagg gcagcccgga ggacaacgag caaaagcagc tgttcgtcga 10741 gcagcacaag cattacctcg acgagatcat cgagcaaatc tccgagttca gcaagcgcgt 10801 gatcctcgcc gacgcgaacc tggataaggt cctctccgcc tacaacaagc accgggacaa 10861 gcccatcaga gagcaagcgg agaacatcat ccatctcttc accctgacga acctcggcgc 10921 tcctgctgct ttcaagtact tcgacaccac gatcgatcgg aagagataca cctccacgaa 10981 ggaggtcctg gacgcgaccc tcatccacca gtcgatcacc ggcctgtacg agacgaggat 11041 cgacctctca caactcggcg gggataagag acccgcagca accaagaagg cagggcaagc 11101 aaagaagaag aagtgactcg agatatgaag atgaagatga aatatttggt gtgtcaaata 11161 aaaagcttgt gtgcttaagt ttgtgttttt ttcttggctt gttgtgttat gaatttgtgg 11221 ctttttctaa tattaaatga atgtaagatc acattataat gaataaacaa atgtttctat 11281 aatccattgt gaatgttttg ttggatctct tctgcagcat ataactactg tatgtgctat 11341 ggtatggact atggaatatg attaaagata aggagctccg gtgacggact ggcactggcc 11401 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca 11461 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc 11521 caacagttgc gcagcctgaa tggcgaatgc tagagcagct tgagcttgga tcagattgtc 11581 gtttcccgcc ttcagtttaa actatcagtg tttgacagga tatattggcg ggtaaaccta 11641 agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc 11701 gttcgtccat ttgtatgtgc atgccaacca cagggttccc ctcgggatca aagtactttg 11761 atccaacccc tccgctgcta tagtgcagtc ggcttctgac gttcagtgca gccgtcttct 11821 gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct gcccttttcc 11881 tggcgttttc ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga ctagaaccgg 11941 agacattacg ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc 12001 accgacgacc aggacttgac caaccaacgg gccgaactgc acgcggccgg ctgcaccaag 12061 ctgttttccg agaagatcac cggcaccagg cgcgaccgcc cggagctggc caggatgctt 12121 gaccacctac gccctggcga cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc 12181 acccgcgacc tactggacat tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc 12241 ctggcagagc cgtgggccga caccaccacg ccggccggcc gcatggtgtt gaccgtgttc 12301 gccggcattg ccgagttcga gcgttcccta atcatcgacc gcacccggag cgggcgcgag 12361 gccgccaagg cccgaggcgt gaagtttggc ccccgcccta ccctcacccc ggcacagatc 12421 gcgcacgccc gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg 12481 cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc gcagcgagga agtgacgccc 12541 accgaggcca ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc cgacgccctg 12601 gcggccgccg agaatgaacg ccaagaggaa caagcatgaa accgcaccag gacggccagg 12661 acgaaccgtt tttcattacc gaagagatcg aggcggagat gatcgcggcc gggtacgtgt 12721 tcgagccgcc cgcgcacggc tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg 12781 atgccaagct ggcggcctgg ccggccagct tggccgctga agaaaccgag cgccgccgtc 12841 taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga 12901 tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa 12961 ccagaaaggc gggtcaggca agacgaccat cgcaacccat ctagcccgcg ccctgcaact 13021 cgccggggcc gatgttctgt tagtcgattc cgatccccag ggcagtgccc gcgattgggc 13081 ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg 13141 cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc 13201 ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag 13261 cccttacgac atatgggcca ccgccgacct ggtggagctg gttaagcagc gcattgaggt 13321 cacggatgga aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat 13381 cggcggtgag gttgccgagg cgctggccgg gtacgagctg cccattcttg agtcccgtat 13441 cacgcagcgc gtgagctacc caggcactgc cgccgccggc acaaccgttc ttgaatcaga 13501 acccgagggc gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact 13561 catttgagtt aatgaggtaa agagaaaatg agcaaaagca caaacacgct aagtgccggc 13621 cgtccgagcg cacgcagcag caaggctgca acgttggcca gcctggcaga cacgccagcc 13681 atgaagcggg tcaactttca gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg 13741 gtacgccaag gcaagaccat taccgagctg ctatctgaat acatcgcgca gctaccagag 13801 taaatgagca aatgaataaa tgagtagatg aattttagcg gctaaaggag gcggcatgga 13861 aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg 13921 ttggccaggc gtaagcggct gggttgcctg ccggccctgc aatggcactg gaacccccaa 13981 gcccgaggaa tcggcgtgag cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg 14041 ctgggtgatg acctggtgga gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc 14101 gaggcagaag cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa 14161 tcccggcaac cgccggcagc cggtgcgccg tcgattagga agccgcccaa gggcgacgag 14221 caaccagatt ttttcgttcc gatgctctat gacgtgggca cccgcgatag tcgcagcatc 14281 atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc 14341 tacgagcttc cagacgggca cgtagaggtt tccgcagggc cggccggcat ggccagtgtg 14401 tgggattacg acctggtact gatggcggtt tcccatctaa ccgaatccat gaaccgatac 14461 cgggaaggga agggagacaa gcccggccgc gtgttccgtc cacacgttgc ggacgtactc 14521 aagttctgcc ggcgagccga tggcggaaag cagaaagacg acctggtaga aacctgcatt 14581 cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa cggccgcctg 14641 gtgacggtat ccgagggtga agccttgatt agccgctaca agatcgtaaa gagcgaaacc 14701 gggcggccgg agtacatcga gatcgagcta gctgattgga tgtaccgcga gatcacagaa 14761 ggcaagaacc cggacgtgct gacggttcac cccgattact ttttgatcga tcccggcatc 14821 ggccgttttc tctaccgcct ggcacgccgc gccgcaggca aggcagaagc cagatggttg 14881 ttcaagacga tctacgaacg cagtggcagc gccggagagt tcaagaagtt ctgtttcacc 14941 gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg atttgaagga ggaggcgggg 15001 caggctggcc cgatcctagt catgcgctac cgcaacctga tcgagggcga agcatccgcc 15061 ggttcctaat gtacggagca gatgctaggg caaattgccc tagcagggga aaaaggtcga 15121 aaaggcctct ttcctgtgga tagcacgtac attgggaacc caaagccgta cattgggaac 15181 cggaacccgt acattgggaa cccaaagccg tacattggga accggtcaca catgtaagtg 15241 actgatataa aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa 15301 actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc ccaagagctg 15361 caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct 15421 atcgcggccg ctggccgctc aaaaatggct ggcctacggc caggcaatct accagggcgc 15481 ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac cctgcctcgc 15541 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaaa cggtcacagc 15601 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg 15661 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt 15721 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg 15781 cacagatgcg taaggagaaa ataccgcatc aggccctctt ccgcttcctc gctcactgac 15841 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 15901 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 15961 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 16021 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 16081 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 16141 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 16201 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 16261 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 16321 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 16381 tat //