LOCUS Exported 10890 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Encodes Y2H Gal4 activation domain for Hsh155 P369E. ACCESSION . VERSION . KEYWORDS pGADT7 Hsh155 P369E SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10890) AUTHORS Carrocci TJ, Zoerner DM, Paulson JC, Hoskins AA TITLE SF3b1 mutations associated with myelodysplastic syndromes alter the fidelity of branchsite selection in yeast. JOURNAL Nucleic Acids Res. 2017 Jan 6. pii: gkw1349. doi: 10.1093/nar/gkw1349. PUBMED 28062854 REFERENCE 2 (bases 1 to 10890) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10890 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 771..1475 /gene="S. cerevisiae ADH1" /label=ADH1 promoter /note="promoter for alcohol dehydrogenase 1" CDS 1521..1541 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 1557..1898 /codon_start=1 /gene="Saccharomyces cerevisiae GAL4 (truncated)" /product="activation domain of the GAL4 transcriptional activator" /label=GAL4 activation domain /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD EDTPPNPKKE" primer_bind 1904..1923 /label=T7 /note="T7 promoter, forward primer" promoter 1904..1922 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 1929..1938 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1941..1967 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" terminator 5318..5505 /gene="S. cerevisiae ADH1" /label=ADH1 terminator /note="transcription terminator for the S. cerevisiae alcohol dehydrogenase 1 (ADH1) gene" primer_bind complement(5373..5397) /label=pHybLex-R /note="ADH terminator, reverse primer" CDS complement(5622..6716) /codon_start=1 /gene="S. cerevisiae LEU2" /product="3-isopropylmalate dehydrogenase, required for leucine biosynthesis" /label=LEU2 /note="yeast auxotrophic marker" /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL IGGAAIEATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG DLGGSNSTTEVGDAVAEEVKKILA" promoter complement(6717..7122) /gene="S. cerevisiae LEU2" /label=LEU2 promoter primer_bind complement(7164..7180) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7164..7180) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(7177..7199) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 7188..7204 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(7212..7242) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7257..7278 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." protein_bind complement(7333..7366) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." protein_bind complement(7444..7477) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." primer_bind complement(7546..7563) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(7717..8305) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(7797..7816) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(8476..9336) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 9099..9118 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(9337..9441) /gene="bla" /label=AmpR promoter primer_bind 9509..9527 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(9565..9587) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 9687..9706 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" rep_origin 9723..10887 /label=2u ori /note="yeast 2u plasmid origin of replication" ORIGIN 1 tgcatgcctg caggtcgaga tccgggatcg aagaaatgat ggtaaatgaa ataggaaatc 61 aaggagcatg aaggcaaaag acaaatataa gggtcgaacg aaaaataaag tgaaaagtgt 121 tgatatgatg tatttggctt tgcggcgccg aaaaaacgag tttacgcaat tgcacaatca 181 tgctgactct gtggcggacc cgcgctcttg ccggcccggc gataacgctg ggcgtgaggc 241 tgtgcccggc ggagtttttt gcgcctgcat tttccaaggt ttaccctgcg ctaaggggcg 301 agattggaga agcaataaga atgccggttg gggttgcgat gatgacgacc acgacaactg 361 gtgtcattat ttaagttgcc gaaagaacct gagtgcattt gcaacatgag tatactagaa 421 gaatgagcca agacttgcga gacgcgagtt tgccggtggt gcgaacaata gagcgaccat 481 gaccttgaag gtgagacgcg cataaccgct agagtacttt gaagaggaaa cagcaatagg 541 gttgctacca gtataaatag acaggtacat acaacactgg aaatggttgt ctgtttgagt 601 acgctttcaa ttcatttggg tgtgcacttt attatgttac aatatggaag ggaactttac 661 acttctccta tgcacatata ttaattaaag tccaatgcta gtagagaagg ggggtaacac 721 ccctccgcgc tcttttccga tttttttcta aaccgtggaa tatttcggat atccttttgt 781 tgtttccggg tgtacaatat ggacttcctc ttttctggca accaaaccca tacatcggga 841 ttcctataat accttcgttg gtctccctaa catgtaggtg gcggagggga gatatacaat 901 agaacagata ccagacaaga cataatgggc taaacaagac tacaccaatt acactgcctc 961 attgatggtg gtacataacg aactaatact gtagccctag acttgatagc catcatcata 1021 tcgaagtttc actacccttt ttccatttgc catctattga agtaataata ggcgcatgca 1081 acttcttttc tttttttttc ttttctctct cccccgttgt tgtctcacca tatccgcaat 1141 gacaaaaaaa tgatggaaga cactaaagga aaaaattaac gacaaagaca gcaccaacag 1201 atgtcgttgt tccagagctg atgaggggta tctcgaagca cacgaaactt tttccttcct 1261 tcattcacgc acactactct ctaatgagca acggtatacg gccttccttc cagttacttg 1321 aatttgaaat aaaaaaaagt ttgctgtctt gctatcaagt ataaatagac ctgcaattat 1381 taatcttttg tttcctcgtc attgttctcg ttccctttct tccttgtttc tttttctgca 1441 caatatttca agctatacca agcatacaat caactccaag ctttgcaaag atggataaag 1501 cggaattaat tcccgagcct ccaaaaaaga agagaaaggt cgaattgggt accgccgcca 1561 attttaatca aagtgggaat attgctgata gctcattgtc cttcactttc actaacagta 1621 gcaacggtcc gaacctcata acaactcaaa caaattctca agcgctttca caaccaattg 1681 cctcctctaa cgttcatgat aacttcatga ataatgaaat cacggctagt aaaattgatg 1741 atggtaataa ttcaaaacca ctgtcacctg gttggacgga ccaaactgcg tataacgcgt 1801 ttggaatcac tacagggatg tttaatacca ctacaatgga tgatgtatat aactatctat 1861 tcgatgatga agatacccca ccaaacccaa aaaaagagat ctttaatacg actcactata 1921 gggcgagcgc cgccatggag tacccatacg acgtaccaga ttacgctcat atggccatgg 1981 aggccagtga attccacccg ggaatgagtc atccgattca atttgtaaac gctaataata 2041 gcgataagag ccaccaatta ggcggtcaat attctattcc tcaggatttg agggaaaacc 2101 tgcagaaaga agctgcacgt attggtgaga acgaaaaaga tgtgctacag gaaaaaatgg 2161 aaactaggac agtgcaaaat cgagaggatt cataccacaa aagaaggttt gatatgaagt 2221 ttgagccgga ttccgataca caaactgtta cgtcttcaga aaatacccaa gacgctgtcg 2281 tgccaaggaa aagaaaatct cgttgggatg taaaagggta cgagccacct gatgaatctt 2341 caacagcggt caaagaaaac tctgatagtg ctttggttaa tgtagaagga atccatgatt 2401 tgatgttttt taaaccttct gaccataaat atttcgccga cgttatatcc aaaaaaccca 2461 tagatgagtt aaataaggat gaaaagaaag aaagaaccct gtcgatgtta cttctgaaaa 2521 taaaaaatgg caatacagcg agtaggagga cttctatgcg gattttaacc gataaagctg 2581 ttacatttgg tccagaaatg atatttaacc gcttattgcc catattacta gacagaagtt 2641 tggaagacca ggaaagacat ctaatgatca agaccataga tcgcgtcctt taccagctag 2701 gagatttaac aaaaccttac gttcacaaaa tacttgtagt agctgcccca ttgctaatag 2761 atgaagatcc aatggttcgt tcaacaggcc aggagataat caccaatctg tctacagtcg 2821 ctggcctaaa aaccattcta acagtaatga gacctgatat tgagaatgag gatgagtatg 2881 ttagaaatgt aacttcaaga gcggcggctg tggtggcaaa agcccttggt gttaatcaat 2941 tgcttccttt catcaatgca gcatgccact cacggaagtc ttggaaagca aggcatactg 3001 gcataaaaat tgtgcagcaa ataggtatac ttttgggaat aggtgtcttg aatcatctta 3061 ctgggctgat gagttgtatc aaagactgcc taatggatga tcacgttgaa gtgcgaattg 3121 ttacagcaca tactttatca acactagcag aaaattctta tccatatggt atcgaagttt 3181 tcaacgttgt actggagcca ttatggaagg gtataagaag tcatcgtggt aaagtgctat 3241 cttctttttt aaaagctgtt ggatctatga tcccattgat ggacccagag tatgccggtt 3301 attataccac agaagcaatg agaataatta gacgtgaatt cgactctccc gatgatgaaa 3361 tgaaaaagac cattctttta gtattacaaa aatgtagcgc tgtagaatcc ataacgccaa 3421 aatttctgag agaagaaatc gcgccagaat tctttcaaaa attttgggtc agacgagtgg 3481 cattggatag accattaaac aaagttgtta catatactac tgttacatta gcaaagaaat 3541 taggttgttc ttataccatt gataaattat taacaccatt gagagatgaa gctgagccgt 3601 tcagaacaat ggctgtccat gctgttacta gaacagtaaa cttattaggg acagcagatt 3661 tagatgagag attagaaacg aggcttattg atgcgctctt gatagcattt caagaacaaa 3721 caaatagtga ttctataatc tttaaagggt ttggggcagt gacggtgtca cttgacatcc 3781 gtatgaaacc gtttttagca cccattgtga gtacgatttt aaatcattta aagcataaaa 3841 ctcccttggt tcgtcaacat gcagcagatc tatgtgccat actaatacca gtcatcaaga 3901 actgtcacga attcgaaatg ttgaataaat tgaacattat attgtatgaa tccttaggcg 3961 aagtatatcc agaggtatta ggttctataa tcaacgctat gtactgtatt actagcgtaa 4021 tggatttgga taaactacaa cctcctatta atcaaatttt accaacctta actccaattt 4081 taagaaacaa gcatagaaaa gtcgaagtaa acactatcaa atttgttgga cttattggta 4141 aattagcacc tacctatgca cctcctaaag aatggatgag aatatgtttt gaactattag 4201 aacttttgaa aagtacaaat aaagaaataa gaagatcagc aaatgccacg tttggattca 4261 ttgctgaagc aattggacct cacgatgttc ttgttgcgtt attgaacaat ttaaaggttc 4321 aagaacgtca gttacgtgta tgcacggctg ttgctatcgg tatcgttgct aaagtgtgtg 4381 gtccttacaa cgtgctgcca gtaatcatga atgaatacac aacaccagag actaatgttc 4441 aaaacggtgt tctcaaggcc atgtcattca tgtttgagta cattggtaac atgtctaagg 4501 attacatata cttcataaca ccattattag aagatgcact caccgatagg gatttagttc 4561 atcgtcagac agcgtcgaac gtaataaccc acttagcttt aaactgttca ggtacaggtc 4621 acgaagatgc cttcattcat ttaatgaatc ttctaatacc caatattttt gaaacttcac 4681 cacatgctat tatgcgtatt ttagaaggtt tggaggcact gagtcaagct cttggccctg 4741 gactattcat gaactacata tgggcaggat tgttccatcc agcgaagaat gtaaggaaag 4801 cattttggag ggtgtataat aacatgtacg taatgtacca ggacgccatg gtaccttttt 4861 accccgttac accagacaac aatgaagaat atatagaaga actggattta gttctgtgag 4921 gatccatcga gctcgagctg cagatgaatc gtagatactg aaaaaccccg caagttcact 4981 tcaactgtgc atcgtgcacc atctcaattt ctttcattta tacatcgttt tgccttcttt 5041 tatgtaacta tactcctcta agtttcaatc ttggccatgt aacctctgat ctatagaatt 5101 ttttaaatga ctagaattaa tgcccatctt ttttttggac ctaaattctt catgaaaata 5161 tattacgagg gcttattcag aagctttgga cttcttcgcc agaggtttgg tcaagtctcc 5221 aatcaaggtt gtcggcttgt ctaccttgcc agaaatttac gaaaagatgg aaaagggtca 5281 aatcgttggt agatacgttg ttgacacttc taaataagcg aatttcttat gatttatgat 5341 ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa gtgactctta 5401 ggttttaaaa cgaaaattct tattcttgag taactctttc ctgtaggtca ggttgctttc 5461 tcaggtatag catgaggtcg ctcttattga ccacacctct accggccggt cgaaattccc 5521 ctaccctatg aacatattcc attttgtaat ttcgtgtcgt ttctattatg aatttcattt 5581 ataaagttta tgtacaaata tcataaaaaa agagaatctt tttaagcaag gattttctta 5641 acttcttcgg cgacagcatc accgacttcg gtggtactgt tggaaccacc taaatcacca 5701 gttctgatac ctgcatccaa aaccttttta actgcatctt caatggcctt accttcttca 5761 ggcaagttca atgacaattt caacatcatt gcagcagaca agatagtggc gatagggttg 5821 accttattct ttggcaaatc tggagcagaa ccgtggcatg gttcgtacaa accaaatgcg 5881 gtgttcttgt ctggcaaaga ggccaaggac gcagatggca acaaacccaa ggaacctggg 5941 ataacggagg cttcatcgga gatgatatca ccaaacatgt tgctggtgat tataatacca 6001 tttaggtggg ttgggttctt aactaggatc atggcggcag aatcaatcaa ttgatgttga 6061 accttcaatg taggaaattc gttcttgatg gtttcctcca cagtttttct ccataatctt 6121 gaagaggcca aaacattagc tttatccaag gaccaaatag gcaatggtgg ctcatgttgt 6181 agggccatga aagcggccat tcttgtgatt ctttgcactt ctggaacggt gtattgttca 6241 ctatcccaag cgacaccatc accatcgtct tcctttctct taccaaagta aatacctccc 6301 actaattctc tgacaacaac gaagtcagta cctttagcaa attgtggctt gattggagat 6361 aagtctaaaa gagagtcgga tgcaaagtta catggtctta agttggcgta caattgaagt 6421 tctttacgga tttttagtaa accttgttca ggtctaacac tacctgtacc ccatttagga 6481 ccacccacag cacctaacaa aacggcatca gccttcttgg aggcttccag cgcctcatct 6541 ggaagtggga cacctgtagc ttcgatagca gcaccaccaa ttaaatgatt ttcgaaatcg 6601 aacttgacat tggaacgaac atcagaaata gctttaagaa ccttaatggc ttcggctgtg 6661 atttcttgac caacgtggtc acctggcaaa acgacgatct tcttaggggc agacattaga 6721 atggtatatc cttgaaatat atatatatat tgctgaaatg taaaaggtaa gaaaagttag 6781 aaagtaagac gattgctaac cacctattgg aaaaaacaat aggtccttaa ataatattgt 6841 caacttcaag tattgtgatg caagcattta gtcatgaacg cttctctatt ctatatgaaa 6901 agccggttcc ggcgctctca cctttccttt ttctcccaat ttttcagttg aaaaaggtat 6961 atgcgtcagg cgacctctga aattaacaaa aaatttccag tcatcgaatt tgattctgtg 7021 cgatagcgcc cctgtgtgtt ctcgttatgt tgaggaaaaa aataatggtt gctaagagat 7081 tcgaactctt gcatcttacg atacctgagt attcccacag ttgggggatc tcgactctag 7141 ctagaggatc aattcgtaat catgtcatag ctgtttcctg tgtgaaattg ttatccgctc 7201 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga 7261 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 7321 tcgtgccagc tgataacttc gtataatgta tgctatacga agttattagg tctgaagagg 7381 agtttacgtc cagccaagct agcttggctg caggtcgagc ggccgcgatc cggaaccctt 7441 aatataactt cgtataatgt atgctatacg aagttatcag ctgcattaat gaatcggcca 7501 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 7561 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 7621 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 7681 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 7741 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 7801 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 7861 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 7921 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 7981 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 8041 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 8101 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac 8161 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 8221 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 8281 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 8341 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 8401 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 8461 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 8521 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 8581 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 8641 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 8701 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 8761 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 8821 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 8881 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 8941 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 9001 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 9061 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 9121 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 9181 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 9241 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 9301 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 9361 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 9421 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac 9481 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc 9541 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc 9601 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg 9661 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca 9721 taacgcattt aagcataaac acgcactatg ccgttcttct catgtatata tatatacagg 9781 caacacgcag atataggtgc gacgtgaaca gtgagctgta tgtgcgcagc tcgcgttgca 9841 ttttcggaag cgctcgtttt cggaaacgct ttgaagttcc tattccgaag ttcctattct 9901 ctagctagaa agtataggaa cttcagagcg cttttgaaaa ccaaaagcgc tctgaagacg 9961 cactttcaaa aaaccaaaaa cgcaccggac tgtaacgagc tactaaaata ttgcgaatac 10021 cgcttccaca aacattgctc aaaagtatct ctttgctata tatctctgtg ctatatccct 10081 atataaccta cccatccacc tttcgctcct tgaacttgca tctaaactcg acctctacat 10141 tttttatgtt tatctctagt attactcttt agacaaaaaa attgtagtaa gaactattca 10201 tagagtgaat cgaaaacaat acgaaaatgt aaacatttcc tatacgtagt atatagagac 10261 aaaatagaag aaaccgttca taattttctg accaatgaag aatcatcaac gctatcactt 10321 tctgttcaca aagtatgcgc aatccacatc ggtatagaat ataatcgggg atgcctttat 10381 cttgaaaaaa tgcacccgca gcttcgctag taatcagtaa acgcgggaag tggagtcagg 10441 ctttttttat ggaagagaaa atagacacca aagtagcctt cttctaacct taacggacct 10501 acagtgcaaa aagttatcaa gagactgcat tatagagcgc acaaaggaga aaaaaagtaa 10561 tctaagatgc tttgttagaa aaatagcgct ctcgggatgc atttttgtag aacaaaaaag 10621 aagtatagat tctttgttgg taaaatagcg ctctcgcgtt gcatttctgt tctgtaaaaa 10681 tgcagctcag attctttgtt tgaaaaatta gcgctctcgc gttgcatttt tgttttacaa 10741 aaatgaagca cagattcttc gttggtaaaa tagcgctttc gcgttgcatt tctgttctgt 10801 aaaaatgcag ctcagattct ttgtttgaaa aattagcgct ctcgcgttgc atttttgttc 10861 tacaaaatga agcacagatg cttcgttgct //