LOCUS       Exported                9404 bp ds-DNA     circular SYN 10-JUN-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    TBX5-FLAG in pLX303
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9404)
  AUTHORS   Rosenbluh J, Nijhawan D, Cox AG, Li X, Neal JT, Schafer EJ, Zack TI,
            Wang X, Tsherniak A, Schinzel AC, Shao DD, Schumacher SE, Weir BA, 
            Vazquez F, Cowley GS, Root DE, Mesirov JP, Beroukhim R, Kuo CJ, 
            Goessling W, Hahn WC
  TITLE     beta-Catenin-Driven Cancers Require a YAP1 Transcriptional Complex 
            for Survival and Tumorigenesis.
  JOURNAL   Cell. 2012 Dec 21;151(7):1457-73. doi: 10.1016/j.cell.2012.11.026. 
            Epub 2012 Dec 13.
  PUBMED    23245941
REFERENCE   2  (bases 1 to 9404)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 10, 2022 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9404
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    531..555
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2155..2178
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2191..2235
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     protein_bind    complement(2280..2304)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    2347..2935
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2400..2420)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2818..2829)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3007..3240
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3318..3439
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3355..3374)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3409..3428
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3479..3614
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3541..3560
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(3634..3653)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3635..3653)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3663..3680)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3663..3679)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3672..3694)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3821..4276
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3908..3927)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4118..4139
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4302..4406
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4407..5267
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4625..4644)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5438..6026
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5927..5946
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6180..6197
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6314..6335
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6350..6380
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6388..6404
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6393..6415
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6412..6428
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6412..6428
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6447..6467
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6449..6467
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6495..6721
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6722..6902
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6949..7074
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7567..7800
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7985..8029
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8204..8704
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8623..8641
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8716..9111
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    9171..9288
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctct ctggctaact gtcgggatca acaagtttgt
      541 acaaaaaagc aggctcttta aaggaaccaa ttcagtcgac gccgccgcga tcgcatggcc
      601 gacgcagacg agggctttgg cctggcgcac acgcctctgg agcctgacgc aaaagacctg
      661 ccctgcgatt cgaaacccga gagcgcgctc ggggccccca gcaagtcccc gtcgtccccg
      721 caggccgcct tcacccagca gggcatggag ggaatcaaag tgtttctcca tgaaagagaa
      781 ctgtggctaa aattccacga agtgggcacg gaaatgatca taaccaaggc tggaaggcgg
      841 atgtttccca gttacaaagt gaaggtgacg ggccttaatc ccaaaacgaa gtacattctt
      901 ctcatggaca ttgtacctgc cgacgatcac agatacaaat tcgcagataa taaatggtct
      961 gtgacgggca aagctgagcc cgccatgcct ggccgcctgt acgtgcaccc agactccccc
     1021 gccaccgggg cgcattggat gaggcagctc gtctccttcc agaaactcaa gctcaccaac
     1081 aaccacctgg acccatttgg gcatattatt ctaaattcca tgcacaaata ccagcctaga
     1141 ttacacatcg tgaaagcgga tgaaaataat ggatttggct caaaaaatac agcgttctgc
     1201 actcacgtct ttcctgagac tgcgtttata gcagtgactt cctaccagaa ccacaagatc
     1261 acgcaattaa agattgagaa taatcccttt gccaaaggat ttcggggcag tgatgacatg
     1321 gagctgcaca gaatgtcaag aatgcaaagt aaagaatatc ccgtggtccc caggagcacc
     1381 gtgaggcaaa aagtggcctc caaccacagt cctttcagca gcgagtctcg agctctctcc
     1441 acctcatcca atttggggtc ccaataccag tgtgagaatg gtgtttccgg cccctcccag
     1501 gacctcctgc ctccacccaa cccataccca ctgccccagg agcatagcca aatttaccat
     1561 tgtaccaaga ggaaagagga agaatgttcc accacagacc atccctataa gaagccctac
     1621 atggagacat cacccagtga agaagattcc ttctaccgct ctagctatcc acagcagcag
     1681 ggcctgggtg cctcctacag gacagagtcg gcacagcggc aagcttgcat gtatgccagc
     1741 tctgcgcccc ccagcgagcc tgtgcccagc ctagaggaca tcagctgcaa cacgtggcca
     1801 agcatgcctt cctacagcag ctgcaccgtc accaccgtgc agcccatgga caggctaccc
     1861 taccagcact tctccgctca cttcacctcg gggcccctgg tccctcggct ggctggcatg
     1921 gccaaccatg gctccccaca gctgggagag ggaatgttcc agcaccagac ctccgtggcc
     1981 caccagcctg tggtcaggca gtgtgggcct cagactggcc tgcagtcccc tggcaccctt
     2041 cagccccctg agttcctcta ctctcatggc gtgccaagga ctctatcccc tcatcagtac
     2101 cactctgtgc acggagttgg catggtgcca gagtggagcg acaatagcac gcgtgactac
     2161 aaagacgatg acgataaagg tgatgggtcc ggcctgaacg acatcttcga ggctcagaaa
     2221 atcgaatggc acgaaggcgc gccgagctcg aggtaggcgg ccgcactcga gatatctaga
     2281 cccagctttc ttgtacaaag tggtttagta atgagctagc gctaaccggt ggcgcgttaa
     2341 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     2401 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     2461 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     2521 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     2581 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     2641 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     2701 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     2761 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     2821 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     2881 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga
     2941 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     3001 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     3061 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     3121 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     3181 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     3241 gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     3301 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     3361 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     3421 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     3481 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     3541 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     3601 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     3661 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     3721 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     3781 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     3841 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     3901 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     3961 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     4021 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     4081 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     4141 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     4201 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     4261 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     4321 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     4381 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     4441 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     4501 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     4561 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     4621 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     4681 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     4741 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     4801 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     4861 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     4921 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     4981 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     5041 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     5101 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     5161 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     5221 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     5281 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     5341 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     5401 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     5461 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     5521 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     5581 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     5641 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     5701 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     5761 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     5821 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     5881 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     5941 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     6001 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     6061 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     6121 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     6181 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     6241 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     6301 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     6361 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     6421 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     6481 agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat
     6541 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt
     6601 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg
     6661 aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa
     6721 cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     6781 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     6841 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     6901 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     6961 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     7021 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     7081 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     7141 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     7201 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     7261 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     7321 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     7381 caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg
     7441 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     7501 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     7561 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     7621 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     7681 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     7741 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     7801 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     7861 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     7921 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     7981 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     8041 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     8101 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     8161 accattatcg tttcagaccc acctcccaac cccgagggga cccttgcgcc ttttccaagg
     8221 cagccctggg tttgcgcagg gacgcggctg ctctgggcgt ggttccggga aacgcagcgg
     8281 cgccgaccct gggtctcgca cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc
     8341 cgctaccctt gtgggccccc cggcgacgct tcctgctccg cccctaagtc gggaaggttc
     8401 cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc gcacgtctca ctagtaccct
     8461 cgcagacgga cagcgccagg gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa
     8521 tagcggctgc tcagcagggc gcgccgagag cagcggccgg gaaggggcgg tgcgggaggc
     8581 ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg cggtgttccg cattctgcaa
     8641 gcctccggag cgcacgtcgg cagtcggctc cctcgttgac cgaatcaccg acctctctcc
     8701 ccagggggta ccaccatggc caagcctttg tctcaagaag aatccaccct cattgaaaga
     8761 gcaacggcta caatcaacag catccccatc tctgaagact acagcgtcgc cagcgcagct
     8821 ctctctagcg acggccgcat cttcactggt gtcaatgtat atcattttac tgggggacct
     8881 tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg cagctggcaa cctgacttgt
     8941 atcgtcgcga tcggaaatga gaacaggggc atcttgagcc cctgcggacg gtgccgacag
     9001 gtgcttctcg atctgcatcc tgggatcaaa gccatagtga aggacagtga tggacagccg
     9061 acggcagttg ggattcgtga attgctgccc tctggttatg tgtgggaggg cctgcagctg
     9121 cagtagtaag aattctagat cttgagacaa atggcagtat tcatccacaa ttttaaaaga
     9181 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     9241 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
     9301 agggacagca gagatccact ttggcgccgg ctcgaggggg cccggggtta ttaatagtaa
     9361 tcaattacgg ggtcattagt tcatagccca tatatggagt tccg
//
