LOCUS       Exported               14616 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Enhancing the lower-part mevalonate pathway; overexpressing yeast 
            mevalonate pathway genes under the control of GAL promoters; 
            integrated into ERG9 locus; .
ACCESSION   .
VERSION     .
KEYWORDS    pIMVAd40R
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14616)
  AUTHORS   Peng B, Nielsen LK, Kampranis SC, Vickers CE
  TITLE     Engineered protein degradation of farnesyl pyrophosphate synthase is
            an effective regulatory mechanism to increase monoterpene production
            in Saccharomyces cerevisiae.
  JOURNAL   Metab Eng. 2018 Feb 19. pii: S1096-7176(17)30214-8. doi: 
            10.1016/j.ymben.2018.02.005.
  PUBMED    29471044
REFERENCE   2  (bases 1 to 14616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14616
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(3948..3959)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(5642..6316)
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(6317..6598)
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     primer_bind     complement(6619..6638)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6738..6760
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6798..6816)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      6857..8199
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    7189..7236
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        8226..8330
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8331..9191
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8549..8568)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9362..9950
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9851..9870
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    10339..10372
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     terminator      10435..10632
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     promoter        complement(11025..11368)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     protein_bind    11423..11456
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     promoter        complement(12679..13120)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(12685..12708)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    13003..13120
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
ORIGIN
        1 caatggctgg atgtaactca ggctgactgg ggtgttagga aagaaaaaga tccggaaact
       61 tatcttgata aataatagtg atcgaattta cgtagcccaa tctaccactt ttttttttca
      121 ttttttaaag tgttatactt agttatgctc taggataatg aactactttt tttttttttt
      181 tttactgtta tcataaatat atatacctta ttgttgtttg caaccgtcgg ttaattcctt
      241 atcaaggttc cccaagttcg gatcattacc atcaatttcc aacattttca tgagttcttc
      301 ttcttcatta ccgtgtttta gggggctgtt cgcacttcta atagggctat caccaagctg
      361 ttctaattcg tccaaaagtt ttcgtgattc tcctgcagtg gaacaagtag aggtgtattc
      421 tgaggaggat gatgagtact ctactgatga cgacgaaacc gaaagtgatg atgaaagtga
      481 tgctagcgat tatactgata tcgatacgga tacagaagct gaagatgatg aatcaccaga
      541 atagatataa gtgattagta tagtttttca ttttagctct tagacgtata tatttcatct
      601 ttataaaaat agatacatgt gtatacaaag caaaaccaaa aaaaaaaaaa aaatttttca
      661 gccatctgtt aagaaatgtg tcactattat gaagtccatg gtaaattcgt gtttcctggc
      721 aataatagat cgtcaatttg ttgctttgtg gtagttttat tttcaaataa ttggaatact
      781 agggatttga ttttaagatc tttattcaaa ttttttgcgc ttaacaaaca gcagccagtc
      841 ccacccaagt ctgtttcaaa tgtctcgtaa ctaaaatcat cttgcaattt ctttttgaaa
      901 ctgtcaattt gctcttgagt aatgtctctt cgtaacaaag tcaaagagca accgccgcca
      961 ccagcaccgg taagttttgt ggagccaatt ctcaaatcat cgctcagatt tttaataagt
     1021 tctaatccag gatgagaaac accgattgag acaagcagtc catgatttat tcttatcaat
     1081 tccaatagtt gttcatacag ttcattatta gtttctacag cctcgtcatc ggtgccttta
     1141 catttactta acttagtcat gatctctaag ccttgtaggg cacattcacc catggcatct
     1201 agaattggct tcataacttc aggaaatttc tcggtgacca acacacgaac gcgagcaaca
     1261 agatcttttg tagaccttgg aattctagta taggttagga tcattggaat ggctgggaaa
     1321 tcatctaaga acttaaaatt gtttgtgttt attgttccat tatgtgagtc tttttcaaat
     1381 agcagggcat taccataagt ggccacagcg ttatctattc ctgaaggggt accgtgaata
     1441 cacttttcac ctatgaaggc ccattgattc actatatgct tatcgttttc tgacagcttt
     1501 tccaagtcat tagatcctat taaccccccc aagtaggcca tagctaaggc cagtgataca
     1561 gaaatagagg cgcttgagcc caacccagca ccgatgggta aagtagactt taaagaaaac
     1621 ttaatattct tggcatgggg gcataggcaa acaaacatat acaggaaaca aaacgctgca
     1681 tggtagtgga aggattcgga tagttgagct aacaacggat ccaaaagact aacgagttcc
     1741 tgagacaagc catcggtggc ttgttgagcc ttggccaatt tttgggagtt tacttgatcc
     1801 tcggtgatgg cattgaaatc attgatggac cacttatgat taaagctaat gtccgggaag
     1861 tccaattcaa tagtatctgg tgcagatgac tcgcttatta gcaggtaggt tctcaacgca
     1921 gacacactag cagcgacggc aggcttgttg tacacagcag agtgttcacc aaaaataata
     1981 acctttcccg gtgcagaagt taagaacggt aatgacatta tgaaagaatt atttttttta
     2041 ttatgttaat cttgtgttta cttaactatt gctattcttg atgataattg aataaggtgc
     2101 ataatgaaga gcaattcaca acaccaaatt ttcaatccaa ttactgattg tttatatatg
     2161 tctacaaaac taatcctatc tccacatttt agcctgcgaa atgtttgttt tttaaacaat
     2221 agctctccag aacattgtat aatttaagaa tatgtgcaca gttaactttc tagcaggagt
     2281 ataatgccat ttgctcccca tcttgagatg ggaagggctt aactaatctc ggttcggagt
     2341 gatccgcccc gatactgcct tctgccttaa tatcgtccaa ggcacatgga cccctgaacg
     2401 gcgcagatat ctccgcacgg acgaaagacc gccggtgcct tcctgaggca accgcccctt
     2461 tcgaatatag atcacgtgac ccatttttag ctactaatag aaaaagaaat tgcaacctac
     2521 ttaagccatt ccggaaggaa gctttccgaa ttgacgttga ataccttttc tgaaaagatt
     2581 ctaccgcatt taattggagt ttaatgcaaa gtagttgcca gttacaagtt gagtgcggct
     2641 acctgatgtt ttccagtcct atattgctgt gatctgaatc tggaaagttc ctctggtccg
     2701 taaacctcct tggattagta catgtttcaa ttacgcagga cccttttacg attgtttttc
     2761 atctctagag gcattttcaa catcgtattt tccgaagcgt tgcataatga aaatgtgata
     2821 ataaggcagt gagctacttt gtcacaacaa agtacgagaa cagatgaggt tccgtggtgt
     2881 ttttggggca actgcattat ttggagcgcc gtatatattt atatcctatt acaaaaaatc
     2941 ataaatgaca ctaaatttga cacctactag tgtcgtcaat cgagaagtag atcctttgtc
     3001 aagttgtgtg ctagtgtctc ccgtcttctg ttcactatta ttcctttggt agaccagtct
     3061 ttgcgtcaat caaagattcg tttgtttctt gtgggcctga accgacttga gttaaaatca
     3121 ctctggcaac atccttttgc aactcaagat ccaattcacg tgcagtaaag ttagatgatt
     3181 caaattgatg gttgaaagcc tcaagctgct cagtagtaaa tttcttgtcc catccaggaa
     3241 cagagccaaa caatttatag ataaatgcaa agagtttcga ctcattttca gctaagtagt
     3301 acaacacagc atttggacct gcatcaaacg tgtatgcaac gattgtttct ccgtaaaact
     3361 gattaatggt gtggcaccaa ctgatgatac gcttggaagt gtcattcatg tagaatattg
     3421 gagggaaaga gtccaaacat gtggcatgga aagagttgga atccatcatt gtttcctttg
     3481 caaaggtggc gaaatctttt tcaacaatgg ctttacgcat gacttcaaat ctctttggta
     3541 cgacatgttc aattctttct ttaaatagtt cggaggttgc cacggtcaat tgcataccct
     3601 gagtggaact cacatccttt ttaatatcgc tgacaactag gacacaagct ttcatctgag
     3661 gccagtcaga gctgtctgcg atttgtactg ccatggaatc atgaccatct tcagcttttc
     3721 ccatttccca ggccacgtat ccgccaaaca acgatctaca agctgaacca gacccctttc
     3781 ttgctattct agatatttct gaagttgact gtggtaattg gtataactta gcaattgcag
     3841 agaccaatgc agcaaagcca gcagcggagg aagctaaacc agctgctgta ggaaagttat
     3901 tttcggagac aatgtggagt ttccattgag ataatgtggg caatgaggcg tccttcgatt
     3961 ccatttcctt tcttaattgg cgtaggtcgc gcagacaatt ttgagttctt tcattgtcga
     4021 tgctgtgtgg ttctccattt aaccacaaag tgtcgcgttc aaactcaggt gcagtagccg
     4081 cagaggtcaa cgttctgagg tcatcttgcg ataaagtcac tgatatggac gaattggtgg
     4141 gcagattcaa cttcgtgtcc cttttccccc aatacttaag ggttgcgatg ttgacgggtg
     4201 cggtaacgga tgctgtgtaa acggtcattt ttgagggaat attcaactgt ttttttttat
     4261 catgttgatg ctctgcataa taatgcccat aaatatttcc gacctgcttt tatatctttg
     4321 ctagccaaac taactgaaca tagctacaca ttattttcag cttggctatt ttgtgaacac
     4381 tgtatagcca gtccttcgga tcacggtcaa cagttgtccg agcgcttttt ggaccctttc
     4441 ccttattttt gggttaagga aaatgacaga aaatatatct aatgagcctt cgctcaacag
     4501 tgctccgaag tatagctttc caaaaggaga ggcaaagcaa tttaagaatg tatgaacaaa
     4561 ataaagggga aaaattaccc cctctacttt accaaacgaa tactaccaat aatatttaca
     4621 acttttcctt atgatttttt cactgaagcg cttcgcaata gttgtgagcg atatcaaaag
     4681 taacgaaatg aacttcgcgg ctcgtgctat attcttgttg ctaccgtcca tatctttcca
     4741 tagattttca atctttgatg tctccatggt ggtacagaga acttgtaaac aattcggtcc
     4801 ctacatgtga ggaaattcgc tgtgacactt ttatcactga actccaaatt taaaaaatag
     4861 cataaaattc gttatacagc aaatctatgt gttgcaatta agaactaaaa gatatagagt
     4921 gcatattttc aagaaggata gtaagctggc aaatcaaaat cattacgacc gagattcccg
     4981 ggtaataact gatataatta aattgaagct ctaatttgtg agtttagtat acatgcattt
     5041 acttataata cagtttttta gttttgctgg ccgcatcttc tcaaatatgc ttcccagcct
     5101 gcttttctgt aacgttcacc ctctacctta gcatcccttc cctttgcaaa tagtcctact
     5161 agtttatcac gctctgtgta aagtgtatat ataataaaac ccaagaagaa cggacaagat
     5221 gatagataaa accatattga acttgtactc ttcttcttgt tgggttggga ccaattcatc
     5281 atcgtatctg gatctttctt taactttcaa gaaaattgga gtttcatttg gcttcacgtt
     5341 aggaggtaat ttatcctggt acatttcttc catgaattgt tcgatcttgg agatttgaat
     5401 gttcaatttt aagaaatttg gatcttgcac agccaattta aataagggat tttgccgatt
     5461 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     5521 atattaacgc ttacaatttc ctgatgcggt attttctcct tacgcatctg tgcggtattt
     5581 cacaccgcat agatcggcaa gtgcacaaac aatacttaaa taaatactac tcagtaataa
     5641 cctatttctt agcatttttg acgaaatttg ctattttgtt agagtctttt acaccatttg
     5701 tctccacacc tccgcttaca tcaacaccaa taacgccatt taatctaagc gcatcaccaa
     5761 cattttctgg cgtcagtcca ccagctaaca taaaatgtaa gctttcgggg ctctcttgcc
     5821 ttccaaccca gtcagaaatc gagttccaat ccaaaagttc acctgtccca cctgcttctg
     5881 aatcaaacaa gggaataaac gaatgaggtt tctgtgaagc tgcactgagt agtatgttgc
     5941 agtcttttgg aaatacgagt cttttaataa ctggcaaacc gaggaactct tggtattctt
     6001 gccacgactc atctccatgc agttggacga tatcaatgcc gtaatcattg accagagcca
     6061 aaacatcctc cttaggttga ttacgaaaca cgccaaccaa gtatttcgga gtgcctgaac
     6121 tatttttata tgcttttaca agacttgaaa ttttccttgc aataaccggg tcaattgttc
     6181 tctttctatt gggcacacat ataataccca gcaagtcagc atcggaatct agagcacatt
     6241 ctgcggcctc tgtgctctgc aagccgcaaa ctttcaccaa tggaccagaa ctacctgtga
     6301 aattaataac agacatactc caagctgcct ttgtgtgctt aatcacgtat actcacgtgc
     6361 tcaatagtca ccaatgccct ccctcttggc cctctccttt tcttttttcg accgaattaa
     6421 ttcttaatcg gcaaaaaaag aaaagctccg gatcaagatt gtacgtaagg tgacaagcta
     6481 tttttcaata aagaatatct tccactactg ccatctggcg tcataactgc aaagtacaca
     6541 tatattacga tgctgttcta ttaaatgctt cctatattat atatatagta atgtcgttga
     6601 tctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     6661 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     6721 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     6781 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     6841 aatggtttct tagtatgatc caatatcaaa ggaaatgata gcattgaagg atgagactaa
     6901 tccaattgag gagtggcagc atatagaaca gctaaagggt agtgctgaag gaagcatacg
     6961 ataccccgca tggaatggga taatatcaca ggaggtacta gactaccttt catcctacat
     7021 aaatagacgc atataagtac gcatttaagc ataaacacgc actatgccgt tcttctcatg
     7081 tatatatata tacaggcaac acgcagatat aggtgcgacg tgaacagtga gctgtatgtg
     7141 cgcagctcgc gttgcatttt cggaagcgct cgttttcgga aacgctttga agttcctatt
     7201 ccgaagttcc tattctctag aaagtatagg aacttcagag cgcttttgaa aaccaaaagc
     7261 gctctgaaga cgcactttca aaaaaccaaa aacgcaccgg actgtaacga gctactaaaa
     7321 tattgcgaat accgcttcca caaacattgc tcaaaagtat ctctttgcta tatatctctg
     7381 tgctatatcc ctatataacc tacccatcca cctttcgctc cttgaacttg catctaaact
     7441 cgacctctac attttttatg tttatctcta gtattactct ttagacaaaa aaattgtagt
     7501 aagaactatt catagagtga atcgaaaaca atacgaaaat gtaaacattt cctatacgta
     7561 gtatatagag acaaaataga agaaaccgtt cataattttc tgaccaatga agaatcatca
     7621 acgctatcac tttctgttca caaagtatgc gcaatccaca tcggtataga atataatcgg
     7681 ggatgccttt atcttgaaaa aatgcacccg cagcttcgct agtaatcagt aaacgcggga
     7741 agtggagtca ggcttttttt atggaagaga aaatagacac caaagtagcc ttcttctaac
     7801 cttaacggac ctacagtgca aaaagttatc aagagactgc attatagagc gcacaaagga
     7861 gaaaaaaagt aatctaagat gctttgttag aaaaatagcg ctctcgggat gcatttttgt
     7921 agaacaaaaa agaagtatag attctttgtt ggtaaaatag cgctctcgcg ttgcatttct
     7981 gttctgtaaa aatgcagctc agattctttg tttgaaaaat tagcgctctc gcgttgcatt
     8041 tttgttttac aaaaatgaag cacagattct tcgttggtaa aatagcgctt tcgcgttgca
     8101 tttctgttct gtaaaaatgc agctcagatt ctttgtttga aaaattagcg ctctcgcgtt
     8161 gcatttttgt tctacaaaat gaagcacaga tgcttcgttc aggtggcact tttcggggaa
     8221 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     8281 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     8341 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     8401 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     8461 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     8521 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     8581 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     8641 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     8701 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     8761 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     8821 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     8881 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     8941 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     9001 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     9061 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     9121 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     9181 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     9241 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     9301 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     9361 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     9421 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     9481 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     9541 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     9601 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     9661 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     9721 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     9781 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     9841 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     9901 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     9961 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg tatttaaatg
    10021 agggaaggtt cagtgaacag gacggcgaaa ctttaatacg atttgggtcg ttacagattg
    10081 acggagagag ggccacattg tttgtcggca agaaacaacg tttactgggg aaggtgacaa
    10141 agctagacgt accgatgggt ataatgcatt tcaattcgaa agataataag gtcgaattag
    10201 ttgatgtaat gaaatataag gttatcttta aggataggcc tctacctatt atgtaagtac
    10261 ttagttattg ttcggagttg tttgtttatg ttatttggcg cagactgcat aggccactag
    10321 tggatctgat atcacctaat aacttcgtat agcatacatt atacgaagtt atattaaggg
    10381 ttctcgagag ctcgttttcg acactggatg gcggcgttag tatcgaatcg acagcagtat
    10441 agcgaccagc attcacatac gattgacgca tgatattact ttctgcgcac ttaacttcgc
    10501 atctgggcag atgatgtcga ggcgaaaaaa aatataaatc acgctaacat ttgattaaaa
    10561 tagaacaact acaatataaa aaaactatac aaatgacaag ttcttgaaaa caagaatctt
    10621 tttattgtca gtactgatca tgagatgcct gcaagcaatt cgttctgtat caggcgcagg
    10681 agcgtcccgt ccgggtcgac caaagcggcc atcgtgcctc cccactcctg cagttcgggg
    10741 gcatggatgc gcggatagcc gctgctggtt tcctggatgc cgacggattt gcactgccgg
    10801 tagaactccg cgaggtcgtc cagcctcagg cagcagctga accaactcgc gaggggatcg
    10861 agcccggggt gggcgaagaa ctccagcatg agatccccgc gctggaggat catccagccg
    10921 gcgtcccgga aaacgattcc gaagcccaac ctttcataga aggcggcggt gggatcgaaa
    10981 tctcgtgatg gcaggttggg cgtcgcttgg tcggtcatac ccatggttgt ttatgttcgg
    11041 atgtgatgtg agaactgtat cctagcaaga ttttaaaagg aagtatatga aagaagaacc
    11101 tcagtggcaa atcctaacct tttatatttc tctacagggg cgcggcgtgg ggacaattca
    11161 acgcgtctgt gaggggagcg tttccctgct cgcaggtctg cagcgaggag ccgtaatttt
    11221 tgcttcgcgc cgtgcggcca tcaaaatgta tggatgcaaa tgattataca tggggatgta
    11281 tgggctaaat gtacgggcga cagtcacatc atgcccctga gctgcgcacg tcaagactgt
    11341 caaggagggt attctgggcc tccatgtcgc tggccgggtg acccggcggg gacgaggcaa
    11401 gctaaacaga tctctagacc taataacttc gtatagcata cattatacga agttatatta
    11461 agggttgtcg acctgcagcg tacgaagctt cagctgtggt agtgccacca cctatgtaag
    11521 ctgctagcat tggtatacgt agaacaacta ggactccctc gtccgctcct tgatacaggt
    11581 agataagttc tgcctgaggg gaggattgag aagagggggt aggggtttgc ttgatagcag
    11641 aataaaagta cagctctagg tctataaaaa aaaattggtt aaagaaaaaa tatacagttt
    11701 tgtatataga atcattaatt aaaaagataa tagtataaaa cacatcagat aaacaaaagt
    11761 ttgcccaata aaattatatt ttccatcaac cagctcacta ttatagcatt ctatgaattt
    11821 gcctgtcatt ttccacttca gaaaggtcat ctaattgctc ccaccagttg aataagtaat
    11881 tctcgcaaat aatcttaaac caaggcgtaa acttgtaact tgggtcagca aacatagttt
    11941 tcaaatcatt tggtgaaacc catttgaagt ctctaacttc attgacgttt gggttgacag
    12001 tcaagttttc tttagcgttg atcttataaa ataggatgta atcaatttca tgttcacccc
    12061 atggttcatt gcttggtgcc atgtaatgga ttctgtttaa aaagtgaaac ttaccccttg
    12121 tcttagtttc atcttctgga atacctaatt catgatctag ttttctcacc gccgcagtaa
    12181 tagcgccctt aatcttatcg tctagcttac ccttcaaacc taattcgtca tcaatacata
    12241 gtggatgaga gcagcatgtg ttagtccaaa gatcagggaa agttattttt tcagtggctc
    12301 tttgttgtaa aagtaattca ccttgttcat tgaaaataaa gacggagaat gcacgatgta
    12361 gtaaaccctt ttcaatattt tccattaaat gacaaacttt cttggtaccg gcaccaatag
    12421 cattatcgtc ccaatccaaa acaatacaat tttcattcat taacttaatt tgctcctcat
    12481 catgaccaga aaaacatgtt tctccgcttt cgtcatttga cgtctcacta gatcgggtat
    12541 taggtctttg ttgtaatgga ataatttcag gaaactcttc caaaatgtct tcaggtgttt
    12601 ggttttgcac taatttggcg taactagata ctgcaccatg gggcatacta ttgttgtcgg
    12661 cagtcattat agttttttct ccttgacgtt aaagtataga ggtatattaa caattttttg
    12721 ttgatacttt tatgacattt gaataagaag taatacaaac cgaaaatgtt gaaagtatta
    12781 gttaaagtgg ttatgcagct tttgcattta tatatctgtt aatagatcaa aaatcatcgc
    12841 ttcgctgatt aattacccca gaaataaggc taaaaaacta atcgcattat tatcctatgg
    12901 ttgttaattt gattcgttga tttgaaggtt tgtggggcca ggttactgcc aatttttcct
    12961 cttcataacc ataaaagcta gtattgtaga atctttattg ttcggagcag tgcggcgcga
    13021 ggcacatctg cgtttcagga acgcgaccgg tgaagaccag gacgcacgga ggagagtctt
    13081 ccgtcggagg gctgtcgccc gctcggcggc ttctaatccg tacttcaata tagcaatgag
    13141 cagttaagcg tattactgaa agttccaaag agaaggtttt tttaggctaa gataatgggg
    13201 ctctttacat ttccacaaca tataagtaag attagatatg gatatgtata tggtggtatt
    13261 gccatgtaat atgattatta aacttctttg cgtccatcca aaaaaaaagt aagaattttt
    13321 gaaaattcaa tataaatgtc agagttgaga gccttcagtg ccccagggaa agcgttacta
    13381 gctggtggat atttagtttt agatccgaaa tatgaagcat ttgtagtcgg attatcggca
    13441 agaatgcatg ctgtagccca tccttacggt tcattgcaag agtctgataa gtttgaagtg
    13501 cgtgtgaaaa gtaaacaatt taaagatggg gagtggctgt accatataag tcctaaaact
    13561 ggcttcattc ctgtttcgat aggcggatct aagaaccctt tcattgaaaa agttatcgct
    13621 aacgtattta gctactttaa gcctaacatg gacgactact gcaatagaaa cttgttcgtt
    13681 attgatattt tctctgatga tgcctaccat tctcaggagg acagcgttac cgaacatcgt
    13741 ggcaacagaa gattgagttt tcattcgcac agaattgaag aagttcccaa aacagggctg
    13801 ggctcctcgg caggtttagt cacagtttta actacagctt tggcctcctt ttttgtatcg
    13861 gacctggaaa ataatgtaga caaatataga gaagttattc ataatttatc acaagttgct
    13921 cattgtcaag ctcagggtaa aattggaagc gggtttgatg tagcggcggc agcatatgga
    13981 tctatcagat atagaagatt cccacccgca ttaatctcta atttgccaga tattggaagt
    14041 gctacttacg gcagtaaact ggcgcatttg gttaatgaag aagactggaa tataacgatt
    14101 aaaagtaacc atttaccttc gggattaact ttatggatgg gcgatattaa gaatggttca
    14161 gaaacagtaa aactggtcca gaaggtaaaa aattggtatg attcgcatat gccggaaagc
    14221 ttgaaaatat atacagaact cgatcatgca aattctagat ttatggatgg actatctaaa
    14281 ctagatcgct tacacgagac tcatgacgat tacagcgatc agatatttga gtctcttgag
    14341 aggaatgact gtacctgtca aaagtatcct gagatcacag aagttagaga tgcagttgcc
    14401 acaattagac gttcctttag aaaaataact aaagaatctg gtgccgatat cgaacctccc
    14461 gtacaaacta gcttattgga tgattgccag accttaaaag gagttcttac ttgcttaata
    14521 cctggtgctg gtggttatga cgccattgca gtgattgcta agcaagatgt tgatcttagg
    14581 gctcaaaccg ctgatgacaa aagattttct aaggtt
//
