LOCUS Exported 10377 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION For the expression of NLS-tdMCP-mCherry. ACCESSION . VERSION . KEYWORDS pHR-TRE3G-NLS-tdMCP-mCherry SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10377) AUTHORS Shao S, Zhang W, Hu H, Xue B, Qin J, Sun C, Sun Y, Wei W, Sun Y TITLE Long-term dual-color tracking of genomic loci by modified sgRNAs of the CRISPR/Cas9 system. JOURNAL Nucleic Acids Res. 2016 May 19;44(9):e86. doi: 10.1093/nar/gkw066. Epub 2016 Feb 4. PUBMED 26850639 REFERENCE 2 (bases 1 to 10377) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10377 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(1..17) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2..18) /label=pBluescriptKS /note="For pBluescript vector" LTR 108..341 /label=3' LTR (Delta-U3) /note="self-inactivating 3' long terminal repeat (LTR) from HIV-1" promoter complement(366..384) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(367..384) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(674..693) /label=pBRrevBam /note="pBR322 vectors, tet region, downstream of BamHI, reverse primer" primer_bind complement(907..926) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 1026..1048 /label=pGEX 3' /note="pGEX vectors, reverse primer" promoter 1172..1276 /gene="bla" /label=AmpR promoter CDS 1277..2137 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(1495..1514) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 2308..2896 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2797..2816 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 3050..3067 /label=L4440 /note="L4440 vector, forward primer" primer_bind complement(3137..3157) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 3142..3471 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 3322..3457 /label=SV40 ori /note="SV40 origin of replication" primer_bind 3384..3403 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" intron 4605..4670 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 4800..4820 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" polyA_signal 5245..5379 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(5282..5301) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 5336..5355 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" LTR 5548..6181 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 6228..6353 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 6850..7082 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 7267..7311 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 7596..7713 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" promoter 7842..8156 /label=tight TRE promoter /note="Tet-responsive promoter PTight, consisting of seven tet operator sequences followed by the minimal CMV promoter" protein_bind 7850..7868 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 7886..7904 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 7921..7939 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 7957..7975 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 7993..8011 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 8028..8046 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" protein_bind 8064..8082 /gene="tetO" /label=tet operator /bound_moiety="tetracycline repressor TetR" /note="bacterial operator O2 for the tetR and tetA genes" primer_bind 8129..8153 /label=LNCX /note="Human CMV promoter, forward primer" CDS 8173..8193 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 8197..8223 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" CDS 8224..8235 /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" regulatory 9010..9019 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 9016..9726 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind complement(9161..9179) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 9429..9448 /label=mCherry-F /note="mCherry, forward primer" misc_feature 9787..10375 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(9840..9860) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(10258..10269) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" ORIGIN 1 cgataccgtc gacctcgagg gaattaattc gagctcggta cctttaagac caatgactta 61 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat 121 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca 181 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag 241 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag 301 atccctcaga cccttttagt cagtgtggaa aatctctagc agcatctaga attaattccg 361 tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg 421 tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg cttactgaag 481 cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc 541 tgagtttctg gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat cagcagggtc 601 atcgctagcc agatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt 661 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc 721 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg 781 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac 841 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg aatggtgcac 901 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc 961 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac 1021 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg 1081 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta 1141 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta 1201 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata 1261 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc 1321 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga 1381 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct 1441 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg 1501 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta 1561 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat 1621 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt 1681 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga 1741 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga 1801 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga 1861 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc 1921 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc 1981 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg 2041 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat 2101 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata 2161 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct 2221 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga 2281 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 2341 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc 2401 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtctttct 2461 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc 2521 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt 2581 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg 2641 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct 2701 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag 2761 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag 2821 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg 2881 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg 2941 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac 3001 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt 3061 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat 3121 tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca 3181 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc 3241 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata 3301 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg 3361 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag 3421 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttgga 3481 cacaagacag gcttgcgaga tatgtttgag aataccactt tatcccgcgt cagggagagg 3541 cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc cagatctcta 3601 taatctcgcg caacctattt tcccctcgaa cactttttaa gccgtagata aacaggctgg 3661 gacacttcac atgagcgaaa aatacatcgt cacctgggac atgttgcaga tccatgcacg 3721 taaactcgca agccgactga tgccttctga acaatggaaa ggcattattg ccgtaagccg 3781 tggcggtctg taccgggtgc gttactggcg cgtgaactgg gtattcgtca tgtcgatacc 3841 gtttgtattt ccagctacga tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca 3901 gaaggcgatg gcgaaggctt catcgttatt gatgacctgg tggataccgg tggtactgcg 3961 gttgcgattc gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc aaaaccggct 4021 ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc aagatacctg gattgaacag 4081 ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc 4141 ctggcactgc cgggcgttgt tctttttaac ttcaggcggg ttacaatagt ttccagtaag 4201 tattctggag gctgcatcca tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg 4261 ctttaaacat cctgaaacct cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc 4321 tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct 4381 gatgtggatc tggcgcggca ttgacccacg cgaaatcctc gacgtccagg cacgtattgt 4441 gatgagcgat gccgaacgta ccgacgatga tttatacgat acggtgattg gctaccgtgg 4501 cggcaactgg atttatgagt gggccccgga tctttgtgaa ggaaccttac ttctgtggtg 4561 tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt 4621 taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct 4681 atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct 4741 cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc 4801 caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt 4861 tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg 4921 aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta 4981 ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt 5041 ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg 5101 ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca 5161 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat 5221 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa 5281 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 5341 ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac 5401 caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta cctagtggtt 5461 tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg 5521 ttaaatatgt actacaaact tagtagttgg aagggctaat tcactcccaa agaagacaag 5581 atatccttga tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca 5641 caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag 5701 ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg 5761 tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc 5821 gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat 5881 atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg 5941 ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact 6001 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca 6061 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg 6121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc 6181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca 6241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc 6301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta 6361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa 6421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc 6481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc 6541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg 6601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc 6661 aaaacaaaag taagaccacc gcacagcaag cggccggccg ctgatcttca gacctggagg 6721 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga 6781 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc 6841 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc 6901 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca 6961 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg 7021 catcaagcag ctccaggcaa gaatcctggc tgtgaaagat acctaaagga tcaacagctc 7081 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct 7141 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac 7201 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag 7261 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg 7321 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg 7381 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat 7441 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga 7501 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct 7561 cgacggtatc gccaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg 7621 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa 7681 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat 7741 ccagtttgga tcgataagct tgatatcgaa ttcgaaacca ttattatcat gacattaacc 7801 tataaaaata ggcgtatcac gaggcccttt cgtcttcact cgagtttact ccctatcagt 7861 gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac 7921 tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta 7981 tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga 8041 tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc 8101 gtgtacggtg ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga 8161 acgcgtatgg gcccaaaaaa gaaaagaaaa gttggctacc cctacgacgt gcccgactac 8221 gccatcgaag gccgccatat gctagccgtt aaaatggctt ctaactttac tcagttcgtt 8281 ctcgtcgaca atggcggaac tggcgacgtg actgtcgccc caagcaactt cgctaacggg 8341 atcgctgaat ggatcagctc taactcgcgt tcacaggctt acaaagtaac ctgtagcgtt 8401 cgtcagagct ctgcgcagaa tcgcaaatac accatcaaag tcgaggtgcc taaaggcgcc 8461 tggcgttcgt acttaaatat ggaactaacc attccaattt tcgccacgaa ttccgactgc 8521 gagcttattg ttaaggcaat gcaaggtctc ctaaaagatg gaaacccgat tccctcagca 8581 atcgcagcaa actccggcat ctacgccatg gcttctaact ttactcagtt cgttctcgtc 8641 gacaatggcg gaactggcga cgtgactgtc gccccaagca acttcgctaa cgggatcgct 8701 gaatggatca gctctaactc gcgttcacag gcttacaaag taacctgtag cgttcgtcag 8761 agctctgcgc agaatcgcaa atacaccatc aaagtcgagg tgcctaaagg cgcctggcgt 8821 tcgtacttaa atatggaact aaccattcca attttcgcca cgaattccga ctgcgagctt 8881 attgttaagg caatgcaagg tctcctaaaa gatggaaacc cgattccctc agcaatcgca 8941 gcaaactccg gcatctacgc ggattctaga atggtgagca agggcgagga gctgttggat 9001 ccaccggtcg ccaccatggt gagcaagggc gaggaggata acatggccat catcaaggag 9061 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag 9121 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag 9181 ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag 9241 gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc 9301 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac 9361 tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc 9421 tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg 9481 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc 9541 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc 9601 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc 9661 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac 9721 aagtaaagcg gccgcgactc tagagtcgac ctgcaggcat gcaagcttga tatcaagctt 9781 atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat 9841 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct 9901 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag 9961 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc 10021 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc 10081 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct 10141 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg 10201 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg 10261 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg 10321 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcat //