LOCUS Exported 10203 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION A Cas9-encoding plasmid containing the 5' portion of the ura4 marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-ura4 system . ACCESSION . VERSION . KEYWORDS pDB4280 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10203) AUTHORS Zhang XR, He JB, Wang YZ, Du LL TITLE A Cloning-Free Method for CRISPR/Cas9-Mediated Genome Editing in Fission Yeast. JOURNAL G3 (Bethesda). 2018 Apr 27. pii: g3.118.200164. doi: 10.1534/g3.118.200164. PUBMED 29703785 REFERENCE 2 (bases 1 to 10203) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10203 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 1..742 /label=adh1 promoter /note="promoter for the Schizosaccharomyces pombe adh1 gene encoding alcohol dehydrogenase" CDS 763..4866 /codon_start=1 /product="Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9 /note="generates RNA-guided double strand breaks in DNA" /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" CDS 4879..4899 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" terminator 4915..5162 /gene="S. cerevisiae CYC1" /label=CYC1 terminator /note="transcription terminator for CYC1" primer_bind complement(4931..4949) /label=CYC1 /note="CYC1 transcription termination signal, reverse primer" primer_bind 5209..5227 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(5265..5287) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 5387..5406 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" rep_origin 5777..6558 /label=ars1 /note="Schizosaccharomyces pombe autonomously replicating sequence ars1" promoter complement(7656..8012) /label=rrk1 promoter/leader /note="promoter and leader RNA for the Schizosaccharomyces pombe rrk1 gene encoding the K-RNA component of RNase P (Jacobs et al., 2014)" primer_bind complement(8049..8065) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(8049..8065) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(8062..8084) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 8073..8089 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(8097..8127) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 8142..8163 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(8280..8297) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(8451..9039) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(8531..8550) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(9210..10070) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 9833..9852 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(10071..10175) /gene="bla" /label=AmpR promoter ORIGIN 1 gccctacaac aactaagaaa atggctatca tgcggaagct ggtgagaaga acagcatcgg 61 gacaagggaa ggaagaacaa agacaaagaa aacaaaagaa agcaattgaa aacaaaacaa 121 aacaattttc attccttctc ttatcattcc ttttcttttc ttttctctca ttcaacgcac 181 tccatcgtat ccgtattcct cttatttttt ctctttctct atatccattt ctttctctct 241 aggtgtgtcc tctctctctc ttcaatttct ctactccgca ttccaacgca tccttccccc 301 aacctcccat ttcctcctta cggcccgata gcgatcgtct ttccctcgct atcactcgct 361 accggcccct cctctgcacc gtaacctcct acgtatttac catatcataa agttttttcc 421 gacgcttatc gctgaccccc tgtcgccctc ctattggctt ccggattatc ttcttgtcca 481 taaggtgatc catgcttcct gaagattccc gaaatgtgtc cactttggcg gggaatcatt 541 ccatccactt ctttctctct cgctttcctc attcggcgct ccccttccgc gtctcattgg 601 tcttccgctc cgtttttgct ttgccgatgt tacttgggga gaggtgcgat aatcctttcg 661 caaaaactcg gtttgacgcc tcccatggta taaatagtgg gtggtggaca ggtgccttcg 721 cttttcttta agcaagagaa ttgctgcagg tcgactctag agatggacaa gaagtactcc 781 attgggctcg atatcggcac aaacagcgtc ggttgggccg tcattacgga cgagtacaag 841 gtgccgagca aaaaattcaa agttctgggc aataccgatc gccacagcat aaagaagaac 901 ctcattggcg ccctcctgtt cgactccggg gagacggccg aagccacgcg gctcaaaaga 961 acagcacggc gcagatatac ccgcagaaag aatcggatct gctacctgca ggagatcttt 1021 agtaatgaga tggctaaggt ggatgactct ttcttccata ggctggagga gtcctttttg 1081 gtggaggagg ataaaaagca cgagcgccac ccaatctttg gcaatatcgt ggacgaggtg 1141 gcgtaccatg aaaagtaccc aaccatatat catctgagga agaagcttgt agacagtact 1201 gataaggctg acttgcggtt gatctatctc gcgctggcgc atatgatcaa atttcgggga 1261 cacttcctca tcgaggggga cctgaaccca gacaacagcg atgtcgacaa actctttatc 1321 caactggttc agacttacaa tcagcttttc gaagagaacc cgatcaacgc atccggagtt 1381 gacgccaaag caatcctgag cgctaggctg tccaaatccc ggcggctcga aaacctcatc 1441 gcacagctcc ctggggagaa gaagaacggc ctgtttggta atcttatcgc cctgtcactc 1501 gggctgaccc ccaactttaa atctaacttc gacctggccg aagatgccaa gcttcaactg 1561 agcaaagaca cctacgatga tgatctcgac aatctgctgg cccagatcgg cgaccagtac 1621 gcagaccttt ttttggcggc aaagaacctg tcagacgcca ttctgctgag tgatattctg 1681 cgagtgaaca cggagatcac caaagctccg ctgagcgcta gtatgatcaa gcgctatgat 1741 gagcaccacc aagacttgac tttgctgaag gcccttgtca gacagcaact gcctgagaag 1801 tacaaggaaa ttttcttcga tcagtctaaa aatggctacg ccggatacat tgacggcgga 1861 gcaagccagg aggaatttta caaatttatt aagcccatct tggaaaaaat ggacggcacc 1921 gaggagctgc tggtaaagct taacagagaa gatctgttgc gcaaacagcg cactttcgac 1981 aatggaagca tcccccacca gattcacctg ggcgaactgc acgctatcct caggcggcaa 2041 gaggatttct accccttttt gaaagataac agggaaaaga ttgagaaaat cctcacattt 2101 cggataccct actatgtagg ccccctcgcc cggggaaatt ccagattcgc gtggatgact 2161 cgcaaatcag aagagaccat cactccctgg aacttcgagg aagtcgtgga taagggggcc 2221 tctgcccagt ccttcatcga aaggatgact aactttgata aaaatctgcc taacgaaaag 2281 gtgcttccta aacactctct gctgtacgag tacttcacag tttataacga gctcaccaag 2341 gtcaaatacg tcacagaagg gatgagaaag ccagcattcc tgtctggaga gcagaagaaa 2401 gctatcgtgg acctcctctt caagacgaac cggaaagtta ccgtgaaaca gctcaaagaa 2461 gactatttca aaaagattga atgtttcgac tctgttgaaa tcagcggagt ggaggatcgc 2521 ttcaacgcat ccctgggaac gtatcacgat ctcctgaaaa tcattaaaga caaggacttc 2581 ctggacaatg aggagaacga ggacattctt gaggacattg tcctcaccct tacgttgttt 2641 gaagataggg agatgattga agaacgcttg aaaacttacg ctcatctctt cgacgacaaa 2701 gtcatgaaac agctcaagag gcgccgatat acaggatggg ggcggctgtc aagaaaactg 2761 atcaatggga tccgagataa gcagtctgga aagacaatcc tggattttct taagtccgat 2821 ggatttgcca accggaactt catgcagttg atccatgatg actctctcac ctttaaggag 2881 gacatccaga aagcacaagt ttctggccag ggggacagtc ttcacgagca catcgctaat 2941 cttgcaggta gcccagctat caaaaaggga atactgcaga ccgttaaggt cgtggatgaa 3001 ctcgtcaaag taatgggaag gcataagccc gagaatatcg ttatcgagat ggcccgagag 3061 aaccaaacta cccagaaggg acagaagaac agtagggaaa ggatgaagag gattgaagag 3121 ggtataaaag aactggggtc ccaaatcctt aaggaacacc cagttgaaaa cacccagctt 3181 cagaatgaga agctctacct gtactacctg cagaacggca gggacatgta cgtggatcag 3241 gaactggaca tcaatcggct ctccgactac gacgtggatc atatcgtgcc ccagtctttt 3301 ctcaaagatg attctattga taataaagtg ttgacaagat ccgataaaaa tagagggaag 3361 agtgataacg tcccctcaga agaagttgtc aagaaaatga aaaattattg gcggcagctg 3421 ctgaacgcca aactgatcac acaacggaag ttcgataatc tgactaaggc tgaacgaggt 3481 ggcctgtctg agttggataa agccggcttc atcaaaaggc agcttgttga gacacgccag 3541 atcaccaagc acgtggccca aattctcgat tcacgcatga acaccaagta cgatgaaaat 3601 gacaaactga ttcgagaggt gaaagttatt actctgaagt ctaagctggt ctcagatttc 3661 agaaaggact ttcagtttta taaggtgaga gagatcaaca attaccacca tgcgcatgat 3721 gcctacctga atgcagtggt aggcactgca cttatcaaaa aatatcccaa gcttgaatct 3781 gaatttgttt acggagacta taaagtgtac gatgttagga aaatgatcgc aaagtctgag 3841 caggaaatag gcaaggccac cgctaagtac ttcttttaca gcaatattat gaattttttc 3901 aagaccgaga ttacactggc caatggagag attcggaagc gaccacttat cgaaacaaac 3961 ggagaaacag gagaaatcgt gtgggacaag ggtagggatt tcgcgacagt ccggaaggtc 4021 ctgtccatgc cgcaggtgaa catcgttaaa aagaccgaag tacagaccgg aggcttctcc 4081 aaggaaagta tcctcccgaa aaggaacagc gacaagctga tcgcacgcaa aaaagattgg 4141 gaccccaaga aatacggcgg attcgattct cctacagtcg cttacagtgt actggttgtg 4201 gccaaagtgg agaaagggaa gtctaaaaaa ctcaaaagcg tcaaggaact gctgggcatc 4261 acaatcatgg agcgatcaag cttcgaaaaa aaccccatcg actttctcga ggcgaaagga 4321 tataaagagg tcaaaaaaga cctcatcatt aagcttccca agtactctct ctttgagctt 4381 gaaaacggcc ggaaacgaat gctcgctagt gcgggcgagc tgcagaaagg taacgagctg 4441 gcactgccct ctaaatacgt taatttcttg tatctggcca gccactatga aaagctcaaa 4501 gggtctcccg aagataatga gcagaagcag ctgttcgtgg aacaacacaa acactacctt 4561 gatgagatca tcgagcaaat aagcgaattc tccaaaagag tgatcctcgc cgacgctaac 4621 ctcgataagg tgctttctgc ttacaataag cacagggata agcccatcag ggagcaggca 4681 gaaaacatta tccacttgtt tactctgacc aacttgggcg cgcctgcagc cttcaagtac 4741 ttcgacacca ccatagacag aaagcggtac acctctacaa aggaggtcct ggacgccaca 4801 ctgattcatc agtcaattac ggggctctat gaaacaagaa tcgacctctc tcagctcggt 4861 ggagacagca gggctgaccc caagaagaag aggaaggtgt gatctcttct cgagtcatgt 4921 aattagttat gtcacgctta cattcacgcc ctccccccac atccgctcta accgaaaagg 4981 aaggagttag acaacctgaa gtctaggtcc ctatttattt ttttatagtt atgttagtat 5041 taagaacgtt atttatattt caaatttttc ttttttttct gtacagacgc gtgtacgcat 5101 gtaacattat actgaaaacc ttgcttgaga aggttttggg acgctcgaag gctttaattt 5161 gcgatccccg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc 5221 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag 5281 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag 5341 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag 5401 attgtactga gagtgcacca taccatcgat ggccaacctt ccaattcatt aaatcgagtc 5461 ctccatcaga tgatttacaa ggttttgcat agaatcccta agtaagagaa gttgttagtg 5521 atttcttcaa gtttactaaa atttacatac aaatggatga tctcttctcc attgtttcct 5581 ttcttcttgc aaacgcgttt tacaaagaga tgacatgcta ttttctgatt atttttttct 5641 atagttttct attttgtacg tccttgggag tccgaaatgt aaaatcgggg tatggttagt 5701 agcggcagcg ttcattatgg tatataaaag ataaaaagta ggggcaagcg agacagaatg 5761 ggatacaagg gcatcgtcta taattatagc taaaaattgt attttaattt gtattttttg 5821 taattttatt tctcattgtt ttacttaaaa tgaatgcgaa ttagagaaaa cttcaacgaa 5881 atgtcaaaat aagctcagca aaatatacaa ttttagggaa agcgatcagc aacttattct 5941 ccgttgtaga ttctttttct tcataaattc caacaatgta actgccaatt gatttttctc 6001 tagatgctaa taaattgaaa ctaatgaata taataacaca ggttttgata aattgtaaac 6061 tcttactcaa aaaaagaatg ttaaaactaa tttaaccttt ttataaaggc tcatggcgtt 6121 aaagaggata gtaaaaattc tacaattttt taatttaatg tcacaattgt cttgcgaata 6181 taattctatt aattttcgct aactgctgtt ttagtactat tactttcaat atgccttata 6241 tgcaacttta attcatgaag aatgcaatat ttttgcaatc tttcttgtac tgagcgcttt 6301 ttatcattta gtactcatta tttaattttt cagtaaaggg caataaataa atttgtagaa 6361 gatgcaatgt aatctcctct atccttttgc tcatatgttt atgagtatac ctagtctaga 6421 aaggcttgta tttaaaatat tattcaataa aaattcacaa tttttacgac atgtgctacc 6481 attcacttaa cttcctgatt ataaaattgg ttcgtttata ctaattactt aagtaccttt 6541 aactaaacaa aatgcctata tatatattaa tttacaatga gtgtcagata agtcactatg 6601 tccgagtggt taaggagtta gactcgccat cgatgagctt agctacaaat cccactggct 6661 atatgtatgc atttgtgtta aaaaagtttg tatagattat ttaatctact cagcattctt 6721 tctctaaata ggaatttgtt acttaatgga gaaaaaaatg tttcgattta cctagtgtat 6781 ttgtttgtat actcacgttt aatttcaaac atccattcta tcttgtgtaa tttttggcat 6841 ggtgaaaaag ataatcagcc ttataatctt tacaaaagta agaaattctg taaataagcc 6901 ttaatgccct tgctttaaat taaaatggtt ctttttcatg ataatgtttg cactttgtga 6961 atatatttta gatagttctg tgaggtataa ttaagatgtt ttagagactt atacaatttt 7021 gtctttataa attcttaatt gattttacca tcccagttta actatgcttc gtcggcatct 7081 ctgcacatgt cgtgttttct taccgtattg tcctaccaag aacctctttt ttgcttggat 7141 cgaaattaaa ggtttaaaag caaagttatg gatgctagag tatttcaaag ctattcagct 7201 agagctgagg ggatgaaaaa tcccattgcc aaggaattgt tggctttgat ggaagaaaag 7261 caaagcaact tgtcagtcgc ggtcgatttg acgaagaaat ccgaaatctt agaattggta 7321 gataaaattg gaccctatgt ctgtgttatc aagacacata ttgacgttgt cgaggatttc 7381 gaccaggata tggtagaaaa actggtggcc ttaggtaaaa agcatcgttt tcttatcttt 7441 gaggatcgca aattcgcaga cattggaaat accgtcaagc tacaatatgc atctggtgtg 7501 tacaaaattg cttcttgggc tcatatcaca aattgccata cagtgccagg cgagggtatt 7561 atacaaggcc tcaaagaagt tggtttacct ttgggacgtg gtctcttgct tttggctgaa 7621 atgtcttcca aaggctcttt ggctactgcg gccgcttctt cggtacaggt tatgtttttt 7681 ggcaacagca actattcaaa tcaactgaaa gttttttaga attcaacaac ttccaatact 7741 caagtgtcca agctagcaaa ctgtttaata atcgtgaccc aacattcctt tcatgcgcac 7801 cctcagcgtc acataaatca acactttaca acacctattc aaagcttcta tatgccttcg 7861 tcatttaaat gtctaacatt tcaaaaaagt gtttgctaat ggtaaattct tgagttttct 7921 tggaagcaca tatataacga gtgtgactgt tctacgctcc ttgctgacta gtcatcagtc 7981 tacgcatgcc aactaccacc aacataagca aaaggtaccc ggggtaccga gctcgaattc 8041 gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac 8101 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca 8161 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat 8221 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc 8281 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 8341 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 8401 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 8461 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 8521 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 8581 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 8641 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 8701 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 8761 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 8821 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 8881 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 8941 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 9001 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 9061 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 9121 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa 9181 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc 9241 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact 9301 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc 9361 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt 9421 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 9481 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg 9541 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt 9601 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc 9661 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt 9721 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc 9781 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc 9841 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa 9901 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac 9961 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa 10021 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt 10081 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa 10141 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct 10201 gac //