LOCUS       Exported                 643 bp ds-DNA     linear   SYN 12-JUN-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pGEX-mE6-AP/2525
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Hatakeyama S, Jensen JP, Weissman AM
  TITLE     Subcellular localization and ubiquitin-conjugating enzyme (E2) 
            interactions of mammalian HECT family ubiquitin protein ligases.
  JOURNAL   J Biol Chem. 1997 Jun 13. 272(24):15085-92.
  PUBMED    9182527
REFERENCE   2  (bases 1 to 643)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             8..25
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 aatcggactg gttccgcgtg gatccccaac tgagggctgt ggaaatgagg cctgcacgaa
       61 tgagttttgt gcttcctgtc caacttttct tcgtatggat aacaatgcag cagctattaa
      121 agcccttgag ctttataaaa ttaatgcaaa actctgtgat cctcatccct ccaagaaagg
      181 agcaagctca gcttaccttg agaactcaaa aggtgcatct aacaactcag agataaaaat
      241 gaacaagaag gaaggaaaag attttaaaga tgtgatttac ctaactgaag agaaagtata
      301 tgaaatttat gaattttgta gagagagtga ggattattcc cctttaattc gtgtaattgg
      361 aagaatattt tctagtgctg aggcactggt tctgagcttt cggaaagtca aacagcacac
      421 aaaggaggaa ttgaaatctc ttcaagaaaa ggatgaagac aaggatgaag atgaaaagga
      481 aaaagctgca tgttctgctg ctgctatgga agaagactca gaagcatctt cttcaaggat
      541 gggtgatagt tcacagggag acaacaatgt acaaaaatta ggtcctgatg atgtgactgt
      601 ggatattgat gctattagaa gggtctacag cagtttgctc gct
//
