LOCUS Exported 756 bp ds-DNA linear SYN 20-MAY-2021 DEFINITION Gateway destination vector for bacterial expression; His,GST tag. ACCESSION . VERSION . KEYWORDS pDest-565 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 756) TITLE Gateway Destination vectors for E. coli expression REFERENCE 2 (bases 1 to 756) AUTHORS . TITLE Direct Submission JOURNAL Exported May 20, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..756 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 37..54 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" CDS 61..708 /codon_start=1 /product="glutathione S-transferase from Schistosoma japonicum" /label=GST /translation="PILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFE LGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGV SRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYM DPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGW?ATFGGGDHPPK" CDS 715..732 /codon_start=1 /product="thrombin recognition and cleavage site" /label=thrombin site /translation="LVPRGS" ORIGIN 1 ttaactttta agaaggagat atacatatcg agatcccatc accatcacca tcacggcgcc 61 cctatactag gttattggaa aattaagggc cttgtgcaac ccactcgact tcttttggaa 121 tatcttgaag aaaaatatga agagcatttg tatgagcgcg atgaaggtga taaatggcga 181 aacaaaaagt ttgaattggg tttggagttt cccaatcttc cttattatat tgatggtgat 241 gttaaattaa cacagtctat ggccatcata cgttatatag ctgacaagca caacatgttg 301 ggtggttgtc caaaagagcg tgcagagatt tcaatgcttg aaggagcggt tttggatatt 361 agatacggtg tttcgagaat tgcatatagt aaagactttg aaactctcaa agttgatttt 421 cttagcaagc tacctgaaat gctgaaaatg ttcgaagatc gtttatgtca taaaacatat 481 ttaaatggtg atcatgtaac ccatcctgac ttcatgttgt atgacgctct tgatgttgtt 541 ttatacatgg acccaatgtg cctggatgcg ttcccaaaat tagtttgttt taaaaaacgt 601 attgaagcta tcccacaaat tgataagtac ttgaaatcca gcaagtatat agcatggcct 661 ttgcagggct ggncagccac gtttggtggt ggcgaccatc ctccaaaatc ggatctggtt 721 ccgcgtggat ctgatatcac aagtttgtac aaaaag //