LOCUS Exported 570 bp ds-DNA linear SYN 12-JUN-2021 DEFINITION 3rd generation lentiviral gRNA plasmid targeting human TTK. ACCESSION . VERSION . KEYWORDS TTK gRNA (BRDN0001147779) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 570) AUTHORS Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, Root DE TITLE Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. JOURNAL Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437. PUBMED 26780180 REFERENCE 2 (bases 1 to 570) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..570 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 56..75 /label=LKO.1 5' /note="Human U6 promoter, forward primer" misc_RNA 154..229 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" misc_feature 285..402 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" ORIGIN 1 tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta 61 tcatatgctt accgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa 121 aggacgaaac accgaaatgc tggaaattgc cctgttttag agctagaaat agcaagttaa 181 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct tttttaagct 241 tggcgtaact agatcttgag acaaatggca gtattcatcc acaattttaa aagaaaaggg 301 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa 361 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac 421 agcagagatc cactttggcg ccggctcgag ggggcccggg tgcaaagatg gataaagttt 481 taaacagaga ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt 541 ggctccggtg cccgtcagtg ggcagagcgc //