LOCUS       Exported                 733 bp ds-DNA     linear   SYN 17-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    H2B-GFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Kanda T, Sullivan KF, Wahl GM
  TITLE     Histone-GFP fusion protein enables sensitive analysis of chromosome 
            dynamics in living mammalian cells.
  JOURNAL   Curr Biol. 1998 Mar 26. 8(7):377-85.
  PUBMED    9545195
REFERENCE   2  (bases 1 to 733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 17, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     regulatory      26..35
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             complement(48..425)
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     promoter        complement(514..717)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(543..563)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 cggtgacgct cctcgccctt gctcaccatg gtggcgaccg gtggatcctt agcgctggtg
       61 tacttggtga tggccttagt accctcggac acggcgtgct tggccaactc cccaggcagc
      121 agcaggcgca cggccgtctg gatctccctg gaggtgatgg tcgagcgctt gttgtaatgc
      181 gccaggcggg aagcctcacc tgcgatgcgc tcgaaaatgt cgttcacaaa cgaattcatg
      241 atgcccatgg ccttggacga aatgccggtg tcagggtgga cctgcttcag aaccttgtac
      301 acatagatgg aatagctctc cttgcggctg cgcttgcgct tcttgccgcc tttcttctgc
      361 gccttagtca ccgccttctt ggagcccttt ttcggggcgg gagcagactt cgctggctct
      421 ggcatggtgg cggtaccgtc gactgcagaa ttcgaagctt gagctcgaga tctgagtccg
      481 gtagcgctag cggatctgac ggttcactaa accagctctg cttatataga cctcccaccg
      541 tacacgccta ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc
      601 ccgttgattt tggtgccaaa acaaactccc attgacgtca atggggtgga gacttggaaa
      661 tccccgtgag tcaaaccgct atccacgccc attgatgtac tgccaaaacc gcatcaccat
      721 ggtaatagcg atg
//
