LOCUS       Exported                 570 bp ds-DNA     linear   SYN 07-SEP-2021
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human FGR.
ACCESSION   .
VERSION     .
KEYWORDS    FGR gRNA (BRDN0001147903)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 7, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     63..82
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        162..237
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    293..410
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa
       61 tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct
      121 tgtggaaagg acgaaacacc gagcttggat tgagtcaaca ggttttagag ctagaaatag
      181 caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt
      241 tttaagcttg gcgtaactag atcttgagac aaatggcagt attcatccac aattttaaaa
      301 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
      361 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
      421 acagggacag cagagatcca ctttggcgcc ggctcgaggg ggcccgggtg caaagatgga
      481 taaagtttta aacagagagg aatctttgca gctaatggac cttctaggtc ttgaaaggag
      541 tgggaattgg ctccggtgcc cgtcagtggg
//
