LOCUS       Exported                 755 bp ds-DNA     linear   SYN 13-JUN-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    pCMV5B-Flag-Smurf2 C716A
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Kavsak P, Rasmussen RK, Causing CG, Bonni S, Zhu H, Thomsen GH, 
            Wrana JL
  TITLE     Smad7 binds to Smurf2 to form an E3 ubiquitin ligase that targets 
            the TGF beta receptor for degradation.
  JOURNAL   Mol Cell. 2000 Dec . 6(6):1365-75.
  PUBMED    11163210
REFERENCE   2  (bases 1 to 755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             57..80
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gagctcgttt agtgaaccgt cagaattgat ctggtaccac gcgtatcgat accatggact
       61 acaaggacga cgatgacaag ggtcgacaat ctaaccccgg acgccggagg aacgggcccg
      121 tcaagctgcg cctgacagta ctctgtgcaa aaaacctggt gaaaaaggat tttttccgac
      181 ttcctgatcc atttgctaag gtggtggttg atggatctgg gcaatgccat tctacagata
      241 ctgtgaagaa tacgcttgat ccaaagtgga atcagcatta tgacctgtat attggaaagt
      301 ctgattcagt tacgatcagt gtatggaatc acaagaagat ccataagaaa caaggtgctg
      361 gatttctcgg ttgtgttcgt cttctttcca atgccatcaa ccgcctcaaa gacactggtt
      421 atcagaggtt ggatttatgc aaactcgggc caaatgacaa tgatacagtt agaggacaga
      481 tagtagtaag tcttcagtcc agagaccgaa taggcacagg aggacaagtt gtggactgca
      541 gtcgtttatt tgataacgat ttaccagacg gctgggaaga aaggagaacc gcctctggaa
      601 gaatccagta tctaaaccat ataacaagaa ctacgcaatg ggagcgccca acacgaccgg
      661 catccgaata ttctagccct ggcagacctc ttagctgctt tgttgatgag aacactccca
      721 ttagtggaac aaatggtgca acatgtggac agtct
//
