LOCUS Exported 570 bp ds-DNA linear SYN 06-JUL-2021 DEFINITION 3rd generation lentiviral gRNA plasmid targeting human NMRK1. ACCESSION . VERSION . KEYWORDS NMRK1 gRNA (BRDN0001148142) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 570) AUTHORS Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, Root DE TITLE Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. JOURNAL Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437. PUBMED 26780180 REFERENCE 2 (bases 1 to 570) AUTHORS . TITLE Direct Submission JOURNAL Exported Jul 6, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..570 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 62..81 /label=LKO.1 5' /note="Human U6 promoter, forward primer" misc_RNA 161..236 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" misc_feature 292..409 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" ORIGIN 1 gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat 61 ggactatcat atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt 121 gtggaaagga cgaaacaccg aaacagtggc aaaacaacac gttttagagc tagaaatagc 181 aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt 241 ttaagcttgg cgtaactaga tcttgagaca aatggcagta ttcatccaca attttaaaag 301 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga 361 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta 421 cagggacagc agagatccac tttggcgccg gctcgagggg gcccgggtgc aaagatggat 481 aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct tgaaaggagt 541 gggaattggc tccggtgccc gtcagtgggc //