LOCUS       Exported                 570 bp ds-DNA     linear   SYN 07-JUL-2021
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human SBK2.
ACCESSION   .
VERSION     .
KEYWORDS    SBK2 gRNA (BRDN0001162192)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 7, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     57..76
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        156..231
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    287..404
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact
       61 atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
      121 aaggacgaaa caccgtgacc gacttcggcc acacggtttt agagctagaa atagcaagtt
      181 aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg cttttttaag
      241 cttggcgtaa ctagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag
      301 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
      361 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
      421 acagcagaga tccactttgg cgccggctcg agggggcccg ggtgcaaaga tggataaagt
      481 tttaaacaga gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa
      541 ttggctccgg tgcccgtcag tgggcagagc
//
