LOCUS Exported 570 bp ds-DNA linear SYN 31-MAY-2021 DEFINITION 3rd generation lentiviral gRNA plasmid targeting human ALPK1. ACCESSION . VERSION . KEYWORDS ALPK1 gRNA (BRDN0001145294) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 570) AUTHORS Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, Root DE TITLE Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. JOURNAL Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437. PUBMED 26780180 REFERENCE 2 (bases 1 to 570) AUTHORS . TITLE Direct Submission JOURNAL Exported May 31, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..570 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 59..78 /label=LKO.1 5' /note="Human U6 promoter, forward primer" misc_RNA 158..233 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" misc_feature 289..406 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" ORIGIN 1 acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga 61 ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg 121 gaaaggacga aacaccgcat cctcgctcgg gactgtggtt ttagagctag aaatagcaag 181 ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgctttttta 241 agcttggcgt aactagatct tgagacaaat ggcagtattc atccacaatt ttaaaagaaa 301 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat 361 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag 421 ggacagcaga gatccacttt ggcgccggct cgagggggcc cgggtgcaaa gatggataaa 481 gttttaaaca gagaggaatc tttgcagcta atggaccttc taggtcttga aaggagtggg 541 aattggctcc ggtgcccgtc agtgggcaga //