LOCUS       Exported                 570 bp ds-DNA     linear   SYN 07-SEP-2021
DEFINITION  3rd generation lentiviral gRNA plasmid targeting human PRKAR2B.
ACCESSION   .
VERSION     .
KEYWORDS    PRKAR2B gRNA (BRDN0001147707)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, 
            Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, 
            Root DE
  TITLE     Optimized sgRNA design to maximize activity and minimize off-target 
            effects of CRISPR-Cas9.
  JOURNAL   Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437.
  PUBMED    26780180
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 7, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     54..73
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        153..228
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    284..401
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa atggactatc
       61 atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
      121 gacgaaacac cgttggtaac tatgataatc gtgttttaga gctagaaata gcaagttaaa
      181 ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt ttttaagctt
      241 ggcgtaacta gatcttgaga caaatggcag tattcatcca caattttaaa agaaaagggg
      301 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
      361 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
      421 gcagagatcc actttggcgc cggctcgagg gggcccgggt gcaaagatgg ataaagtttt
      481 aaacagagag gaatctttgc agctaatgga ccttctaggt cttgaaagga gtgggaattg
      541 gctccggtgc ccgtcagtgg gcagagcgca
//
