LOCUS Exported 570 bp ds-DNA linear SYN 06-JUL-2021 DEFINITION 3rd generation lentiviral gRNA plasmid targeting human CAMK2G. ACCESSION . VERSION . KEYWORDS CAMK2G gRNA (BRDN0001147974) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 570) AUTHORS Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, Root DE TITLE Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9. JOURNAL Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437. PUBMED 26780180 REFERENCE 2 (bases 1 to 570) AUTHORS . TITLE Direct Submission JOURNAL Exported Jul 6, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..570 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 54..73 /label=LKO.1 5' /note="Human U6 promoter, forward primer" misc_RNA 153..228 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" misc_feature 284..401 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" ORIGIN 1 gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa atggactatc 61 atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag 121 gacgaaacac cgctcagggg acaagtaacc tggttttaga gctagaaata gcaagttaaa 181 ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt ttttaagctt 241 ggcgtaacta gatcttgaga caaatggcag tattcatcca caattttaaa agaaaagggg 301 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa 361 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca 421 gcagagatcc actttggcgc cggctcgagg gggcccgggt gcaaagatgg ataaagtttt 481 aaacagagag gaatctttgc agctaatgga ccttctaggt cttgaaagga gtgggaattg 541 gctccggtgc ccgtcagtgg gcagagcgca //