LOCUS Exported 10959 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains KanMx4 marker for yeast transformation.. ACCESSION . VERSION . KEYWORDS pML104-KanMx4 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10959) TITLE Patricia Wittkopp yeast CRISPR plasmids JOURNAL Unpublished REFERENCE 2 (bases 1 to 10959) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10959 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 41..59 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(97..119) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 219..238 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" gene 427..1783 /label=kanMX /note="yeast selectable marker conferring kanamycin resistance (Wach et al., 1994)" promoter 427..770 /label=TEF promoter /note="Ashbya gossypii TEF promoter" CDS 771..1580 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin" /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(838..857) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" terminator 1586..1783 /label=TEF terminator /note="Ashbya gossypii TEF terminator" promoter 1858..2524 /gene="S. cerevisiae TDH3" /label=GAP promoter /note="promoter for glyceraldehyde-3-phosphate dehydrogenase" primer_bind 2394..2417 /label=GPDpro-F /note="S. cerevisiae GPD promoter, forward primer" regulatory 2553..2562 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 2559..4277 /codon_start=1 /product="N-terminal portion of Streptococcus pyogenes Cas9 (Zetsche et al., 2015)" /label=Cas9(N) /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE" CDS 6672..6692 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" terminator 6720..6907 /gene="S. cerevisiae ADH1" /label=ADH1 terminator /note="transcription terminator for the S. cerevisiae alcohol dehydrogenase 1 (ADH1) gene" primer_bind complement(6775..6799) /label=pHybLex-R /note="ADH terminator, reverse primer" terminator 7025..7044 /label=SUP4 terminator /note="transcription terminator for the S. cerevisiae SUP4 tRNA gene" promoter complement(7126..7394) /label=SNR52 promoter /note="promoter for the S. cerevisiae small nucleolar RNA gene SNR52" primer_bind complement(7425..7445) /label=T3 /note="T3 promoter, forward primer" promoter complement(7425..7443) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(7464..7480) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7464..7480) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(7477..7499) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 7488..7504 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(7512..7542) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 7557..7578 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(7695..7712) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(7866..8454) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(7946..7965) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(8625..9485) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 9248..9267 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(9486..9590) /gene="bla" /label=AmpR promoter rep_origin 9617..10959 /label=2u ori /note="yeast 2u plasmid origin of replication" protein_bind complement(10580..10627) /label=FRT /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." ORIGIN 1 atactaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc 61 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga 121 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc 181 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact 241 gagagtgcac cataccacct tttcaattca tcattttttt tttattcttt tttttgattt 301 cggtttcctt gaaatttttt tgattcggta atctccgaac agaaggaaga acgaaggaag 361 gagcacagac ttagattggt atatatacgc aagcttgcct cgtccccgcc gggtcacccg 421 gccagcgaca tggaggccca gaataccctc cttgacagtc ttgacgtgcg cagctcaggg 481 gcatgatgtg actgtcgccc gtacatttag cccatacatc cccatgtata atcatttgca 541 tccatacatt ttgatggccg cacggcgcga agcaaaaatt acggctcctc gctgcagacc 601 tgcgagcagg gaaacgctcc cctcacagac gcgttgaatt gtccccacgc cgcgcccctg 661 tagagaaata taaaaggtta ggatttgcca ctgaggttct tctttcatat acttcctttt 721 aaaatcttgc taggatacag ttctcacatc acatccgaac ataaacaacc atgggtaagg 781 aaaagactca cgtttcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt 841 ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcga ttgtacggga 901 agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta 961 cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc 1021 attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc ggcaaaacag 1081 cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag 1141 tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg 1201 tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt 1261 ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataagcttt 1321 tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt 1381 ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat 1441 accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac 1501 ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga 1561 tgctcgatga gtttttctaa tcagtactga caataaaaag attcttgttt tcaagaactt 1621 gtcatttgta tagttttttt atattgtagt tgttctattt taatcaaatg ttagcgtgat 1681 ttatattttt tttcgcctcg acatcatctg cccagatgcg aagttaagtg cgcagaaagt 1741 aatatcatgc gtcaatcgta tgtgaatgct ggtcgctata ctgctgtcga ttcgatacta 1801 acgccgcctc cagtgtcgaa tgatatcgaa ttcagtctac tagtcagttc gagtttatca 1861 ttatcaatac tgccatttca aagaatacgt aaataattaa tagtagtgat tttcctaact 1921 ttatttagtc aaaaaattag ccttttaatt ctgctgtaac ccgtacatgc ccaaaatagg 1981 gggcgggtta cacagaatat ataacatcgt aggtgtctgg gtgaacagtt tattcctggc 2041 atccactaaa tataatggag cccgcttttt aagctggcat ccagaaaaaa aaagaatccc 2101 agcaccaaaa tattgttttc ttcaccaacc atcagttcat aggtccattc tcttagcgca 2161 actacagaga acaggggcac aaacaggcaa aaaacgggca caacctcaat ggagtgatgc 2221 aacctgcctg gagtaaatga tgacacaagg caattgaccc acgcatgtat ctatctcatt 2281 ttcttacacc ttctattacc ttctgctctc tctgatttgg aaaaagctga aaaaaaaggt 2341 tgaaaccagt tccctgaaat tattccccta cttgactaat aagtatataa agacggtagg 2401 tattgattgt aattctgtaa atctatttct taaacttctt aaattctact tttatagtta 2461 gtcttttttt tagttttaaa acaccaagaa cttagtttcg aataaacaca cataaacaaa 2521 caaaagatct gatatggatc gaattagatc tcgccaccat ggacaagaag tattctatcg 2581 gactggacat cgggactaat agcgtcgggt gggccgtcat cactgacgag tacaaggtgc 2641 cctctaagaa gttcaaggtg ctcgggaaca ccgaccggca ttccatcaag aaaaatctga 2701 tcggagctct cctctttgat tcaggggaga ccgctgaagc aacccgcctc aagcggactg 2761 ctagacggcg gtacaccagg aggaagaacc ggatttgtta ccttcaagag atattctcca 2821 acgaaatggc aaaggtcgac gacagcttct tccataggct ggaagaatca ttcctcgtgg 2881 aagaggataa gaagcatgaa cggcatccca tcttcggtaa tatcgtcgac gaggtggcct 2941 atcacgagaa atacccaacc atctaccatc ttcgcaaaaa gctggtggac tcaaccgaca 3001 aggcagacct ccggcttatc tacctggccc tggcccacat gattaagttc agaggccact 3061 tcctgatcga gggcgacctc aatcctgaca atagcgatgt ggataaactg ttcatccagc 3121 tggtgcagac ttacaaccag ctctttgaag agaaccccat caatgcaagc ggagtcgatg 3181 ccaaggccat tctgtcagcc cggctgtcaa agagccgcag acttgagaat cttatcgctc 3241 agctgccggg tgaaaagaaa aatggactgt tcgggaacct gattgctctt tcacttgggc 3301 tgactcccaa tttcaagtct aatttcgacc tggcagagga tgccaagctg caactgtcca 3361 aggacaccta tgatgacgat ctcgacaacc tcctggccca gatcggtgac caatacgccg 3421 accttttcct tgctgctaag aatctttctg acgccatcct gctgtctgac attctccgcg 3481 tgaacactga aatcaccaag gcccctcttt cagcttcaat gattaagcgg tatgatgagc 3541 accaccagga cctgaccctg cttaaggcac tcgtccggca gcagcttccg gagaagtaca 3601 aggaaatctt ctttgaccag tcaaagaatg gatacgccgg ctacatcgac ggaggtgcct 3661 cccaagagga attttataag tttatcaaac ctatccttga gaagatggac ggcaccgaag 3721 agctcctcgt gaaactgaat cgggaggatc tgctgcggaa gcagcgcact ttcgacaatg 3781 ggagcattcc ccaccagatc catcttgggg agcttcacgc catccttcgg cgccaagagg 3841 acttctaccc ctttcttaag gacaacaggg agaagattga gaaaattctc actttccgca 3901 tcccctacta cgtgggaccc ctcgccagag gaaatagccg gtttgcttgg atgaccagaa 3961 agtcagaaga aactatcact ccctggaact tcgaagaggt ggtggacaag ggagccagcg 4021 ctcagtcatt catcgaacgg atgactaact tcgataagaa cctccccaat gagaaggtcc 4081 tgccgaaaca ttccctgctc tacgagtact ttaccgtgta caacgagctg accaaggtga 4141 aatatgtcac cgaagggatg aggaagcccg cattcctgtc aggcgaacaa aagaaggcaa 4201 ttgtggacct tctgttcaag accaatagaa aggtgaccgt gaagcagctg aaggaggact 4261 atttcaagaa aattgaatgc ttcgactctg tggagattag cggggtcgaa gatcggttca 4321 acgcaagcct gggtacctac catgatctgc ttaagatcat caaggacaag gattttctgg 4381 acaatgagga gaaagaggac atccttgagg acattgtcct gactctcact ctgttcgagg 4441 accgggaaat gatcgaggag aggcttaaga cctacgccca tctgttcgac gataaagtga 4501 tgaagcaact taaacggaga agatataccg gatggggacg ccttagccgc aaactcatca 4561 acggaatccg ggacaaacag agcggaaaga ccattcttga tttccttaag agcgacggat 4621 tcgctaatcg caacttcatg caacttatcc atgatgattc cctgaccttt aaggaggaca 4681 tccagaaggc ccaagtgtct ggacaaggtg actcactgca cgagcatatc gcaaatctgg 4741 ctggttcacc cgctattaag aagggtattc tccagaccgt gaaagtcgtg gacgagctgg 4801 tcaaggtgat gggtcgccat aaaccagaga acattgtcat cgagatggcc agggaaaacc 4861 agactaccca gaagggacag aagaacagca gggagcggat gaaaagaatt gaggaaggga 4921 ttaaggagct cgggtcacag atccttaaag agcacccggt ggaaaacacc cagcttcaga 4981 atgagaagct ctatctgtac taccttcaaa atggacgcga tatgtatgtg gaccaagagc 5041 ttgatatcaa caggctctca gactacgacg tggaccacat cgtccctcag agcttcctca 5101 aagacgactc aattgacaat aaggtgctga ctcgctcaga caagaaccgg ggaaagtcag 5161 ataacgtgcc ctcagaggaa gtcgtgaaaa agatgaagaa ctattggcgc cagcttctga 5221 acgcaaagct aatcactcag cggaagttcg acaatctcac taaggctgag aggggcggac 5281 tgagcgaact ggacaaagca ggattcatta aacggcaact tgtggagact cggcagatta 5341 ctaaacatgt agcccaaatc cttgactcac gcatgaatac caagtacgac gaaaacgaca 5401 aacttatccg cgaggtgaag gtgattaccc tgaagtccaa gctggtcagc gatttcagaa 5461 aggactttca attctacaaa gtgcgggaga tcaataacta tcatcatgct catgacgcat 5521 atctgaatgc cgtggtggga accgccctaa tcaagaagta cccaaagctg gaaagcgagt 5581 tcgtgtacgg agactacaag gtctacgacg tgcgcaagat gattgccaaa tctgagcagg 5641 agatcggaaa ggccaccgca aagtacttct tctacagcaa catcatgaat ttcttcaaga 5701 ccgaaatcac ccttgcaaac ggtgagatcc ggaagaggcc gctcatcgag actaatgggg 5761 agactggcga aatcgtgtgg gacaagggca gagatttcgc taccgtgcgc aaagtgcttt 5821 ctatgcctca agtgaacatc gtgaagaaaa ccgaggtgca aaccggaggc ttttctaagg 5881 aatcaatcct ccccaagcgc aactccgaca agctcattgc aaggaagaag gattgggacc 5941 ctaagaagta cggcggattc gattcaccaa ctgtggctta ttctgtcctg gtcgtggcta 6001 aggtggaaaa aggaaagtct aagaagctca agagcgtgaa ggaactgctg ggtatcacca 6061 ttatggagcg cagctccttc gagaagaacc caattgactt tctcgaagcc aaaggttaca 6121 aggaagtcaa gaaggacctt atcatcaagc tcccaaagta tagcctgttc gaactggaga 6181 atgggcggaa gcggatgctc gcctccgctg gcgaacttca gaagggtaat gagctggctc 6241 tcccctccaa gtacgtgaat ttcctctacc ttgcaagcca ttacgagaag ctgaagggga 6301 gccccgagga caacgagcaa aagcaactgt ttgtggagca gcataagcat tatctggacg 6361 agatcattga gcagatttcc gagttttcta aacgcgtcat tctcgctgat gccaacctcg 6421 ataaagtcct tagcgcatac aataagcaca gagacaaacc aattcgggag caggctgaga 6481 atatcatcca cctgttcacc ctcaccaatc ttggtgcccc tgccgcattc aagtacttcg 6541 acaccaccat cgaccggaaa cgctatacct ccaccaaaga agtgctggac gccaccctca 6601 tccaccagag catcaccgga ctttacgaaa ctcggattga cctctcacag ctcggagggg 6661 atgagggagc tcccaagaaa aagcgcaagg taggtagttc cggatcctag actctcgagg 6721 cgaatttctt atgatttatg atttttatta ttaaataagt tataaaaaaa ataagtgtat 6781 acaaatttta aagtgactct taggttttaa aacgaaaatt cttattcttg agtaactctt 6841 tcctgtaggt caggttgctt tctcaggtat agcatgaggt cgctcttatt gaccacacct 6901 ctaccggcat gccgagcaaa tgcctgcaaa tcgctcccca tttctctaga gcggccgtgg 6961 tatcgtttag attggcaatt acagtgtctt agctcacatg cttataacta attacatgac 7021 tcgaagacat aaaaaacaaa aaaagcacca ccgactcggt gccacttttt caagttgata 7081 acggactagc cttattttaa cttgctattt aaatgcatcg attctgatca tttatctttc 7141 actgcggaga agtttcgaac gccgaaacat gcgcaccaac tttcacttct acagcgtttg 7201 accaaaatct tttgaacaga acattgtagg gtgtgaaaaa atgcgcacct ttaccgctag 7261 cccaagaggg cactacaaaa tctagagttg tacttcaaac gtacatgtaa tcaccttgta 7321 tatactcgaa agaaaacatc aagtttctgt ataaatatga gtgaaagcat aatcatacat 7381 tatcttttca aagattgtcc gcggtggagc tccagctttt gttcccttta gtgagggtta 7441 attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc 7501 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga 7561 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 7621 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 7681 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 7741 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 7801 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 7861 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 7921 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 7981 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 8041 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 8101 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 8161 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 8221 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 8281 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca 8341 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 8401 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 8461 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 8521 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 8581 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 8641 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 8701 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 8761 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 8821 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 8881 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 8941 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 9001 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 9061 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 9121 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 9181 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 9241 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 9301 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 9361 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 9421 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 9481 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 9541 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 9601 cgaaaagtgc cacctgaacg aagcatctgt gcttcatttt gtagaacaaa aatgcaacgc 9661 gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa 9721 cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca tttttgtaaa acaaaaatgc 9781 aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa 9841 tgcaacgcga gagcgctatt ttaccaacaa agaatctata cttctttttt gttctacaaa 9901 aatgcatccc gagagcgcta tttttctaac aaagcatctt agattacttt ttttctcctt 9961 tgtgcgctct ataatgcagt ctcttgataa ctttttgcac tgtaggtccg ttaaggttag 10021 aagaaggcta ctttggtgtc tattttctct tccataaaaa aagcctgact ccacttcccg 10081 cgtttactga ttactagcga agctgcgggt gcattttttc aagataaagg catccccgat 10141 tatattctat accgatgtgg attgcgcata ctttgtgaac agaaagtgat agcgttgatg 10201 attcttcatt ggtcagaaaa ttatgaacgg tttcttctat tttgtctcta tatactacgt 10261 ataggaaatg tttacatttt cgtattgttt tcgattcact ctatgaatag ttcttactac 10321 aatttttttg tctaaagagt aatactagag ataaacataa aaaatgtaga ggtcgagttt 10381 agatgcaagt tcaaggagcg aaaggtggat gggtaggtta tatagggata tagcacagag 10441 atatatagca aagagatact tttgagcaat gtttgtggaa gcggtattcg caatatttta 10501 gtagctcgtt acagtccggt gcgtttttgg ttttttgaaa gtgcgtcttc agagcgcttt 10561 tggttttcaa aagcgctctg aagttcctat actttctaga gaataggaac ttcggaatag 10621 gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag ctgcgcacat 10681 acagctcact gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat atatacatga 10741 gaagaacggc atagtgcgtg tttatgctta aatgcgtact tatatgcgtc tatttatgta 10801 ggatgaaagg tagtctagta cctcctgtga tattatccca ttccatgcgg ggtatcgtat 10861 gcttccttca gcactaccct ttagctgttc tatatgctgc cactcctcaa ttggattagt 10921 ctcatccttc aatgctatca tttcctttga tattggatc //