LOCUS Exported 6044 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION B108 enhancer of Blimp1 gene drives Otx2 (isoform b) and Cre expression.. ACCESSION . VERSION . KEYWORDS B108-Otx2-IRES-Cre SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6044) AUTHORS Wang S, Sengel C, Emerson MM, Cepko CL TITLE A gene regulatory network controls the binary fate decision of rod and bipolar cells in the vertebrate retina. JOURNAL Dev Cell. 2014 Sep 8;30(5):513-27. doi: 10.1016/j.devcel.2014.07.018. Epub 2014 Aug 21. PUBMED 25155555 REFERENCE 2 (bases 1 to 6044) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6044 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(161..178) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 388..407 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 581..1609 /codon_start=1 /product="site-specific recombinase" /label=Cre /note="Cre is a site-specific recombinase from bacteriophage P1. Recombination occurs at loxP sequences." /translation="MANLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE DGD" CDS 1619..1648 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" primer_bind complement(1744..1763) /label=Bglob-pA-R /note="Rabbit beta-globin polyA region, reverse primer" polyA_signal 1809..1864 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" primer_bind complement(1863..1882) /label=rbglobpA-R /note="Rabbit beta-globin polyA, reverse primer. Also called rb-glob-pA-term-R" primer_bind complement(2225..2241) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2225..2241) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(2238..2260) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 2249..2265 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(2273..2303) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2318..2339 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 2398..2593 /label=SV40 promoter /note="SV40 early promoter" rep_origin 2444..2579 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2506..2525 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" polyA_signal 2599..2733 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2636..2655) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 2690..2709 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(2801..2818) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(2972..3560) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(3052..3071) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(3731..4591) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 4354..4373 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(4592..4696) /gene="bla" /label=AmpR promoter polyA_signal 4769..4817 /note="synthetic polyadenylation signal" misc_feature 4831..4922 /label=pause site /note="RNA polymerase II transcriptional pause signal from the human alpha-2 globin gene" primer_bind 4871..4890 /label=RVprimer3 /note="pGL3 vector, forward primer" misc_feature 6038..580 /label=IRES2 /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" ORIGIN 1 ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt 61 gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct 121 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa 181 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg 241 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc 301 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg 361 agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg 421 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc 481 tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg 541 gttttccttt gaaaaacacg atgataatat ggccacaacc atggccaatt tactgaccgt 601 acaccaaaat ttgcctgcat taccggtcga tgcaacgagt gatgaggttc gcaagaacct 661 gatggacatg ttcagggatc gccaggcgtt ttctgagcat acctggaaaa tgcttctgtc 721 cgtttgccgg tcgtgggcgg catggtgcaa gttgaataac cggaaatggt ttcccgcaga 781 acctgaagat gttcgcgatt atcttctata tcttcaggcg cgcggtctgg cagtaaaaac 841 tatccagcaa catttgggcc agctaaacat gcttcatcgt cggtccgggc tgccacgacc 901 aagtgacagc aatgctgttt cactggttat gcggcggatc cgaaaagaaa acgttgatgc 961 cggtgaacgt gcaaaacagg ctctagcgtt cgaacgcact gatttcgacc aggttcgttc 1021 actcatggaa aatagcgatc gctgccagga tatacgtaat ctggcatttc tggggattgc 1081 ttataacacc ctgttacgta tagccgaaat tgccaggatc agggttaaag atatctcacg 1141 tactgacggt gggagaatgt taatccatat tggcagaacg aaaacgctgg ttagcaccgc 1201 aggtgtagag aaggcactta gcctgggggt aactaaactg gtcgagcgat ggatttccgt 1261 ctctggtgta gctgatgatc cgaataacta cctgttttgc cgggtcagaa aaaatggtgt 1321 tgccgcgcca tctgccacca gccagctatc aactcgcgcc ctggaaggga tttttgaagc 1381 aactcatcga ttgatttacg gcgctaagga tgactctggt cagagatacc tggcctggtc 1441 tggacacagt gcccgtgtcg gagccgcgcg agatatggcc cgcgctggag tttcaatacc 1501 ggagatcatg caagctggtg gctggaccaa tgtaaatatt gtcatgaact atatccgtaa 1561 cctggatagt gaaacagggg caatggtgcg cctgctggaa gatggcgatg gaccggtgga 1621 acaaaaactt atttctgaag aagatctgtg aatcgcggcc ggccgcactc ctcaggtgca 1681 ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg 1741 agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac 1801 ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct 1861 ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt 1921 ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag 1981 aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt 2041 gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct 2101 aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt 2161 catagctgtc cctcttctct tatggagatc cctcgacctg cagcccaagc ttggcgtaat 2221 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac 2281 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa 2341 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag cggatccgca 2401 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc 2461 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc 2521 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct 2581 aggcttttgc aaaaagctaa cttgtttatt gcagcttata atggttacaa ataaagcaat 2641 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc 2701 aaactcatca atgtatctta tcatgtctgg atccgctgca ttaatgaatc ggccaacgcg 2761 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 2821 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 2881 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 2941 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 3001 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 3061 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 3121 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta 3181 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 3241 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 3301 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 3361 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat 3421 ttggtatctg cgctctgctg aagtcagtta ccttcggaaa aagagttggt agctcttgat 3481 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 3541 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 3601 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 3661 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 3721 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 3781 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 3841 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 3901 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 3961 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 4021 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 4081 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 4141 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 4201 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 4261 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 4321 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 4381 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 4441 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 4501 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 4561 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 4621 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 4681 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgggtcgaga agtaatatta 4741 aggtacggga ggtacttgga gcggccgcaa taaaatatct ttattttcat tacatctgtg 4801 tgttggtttt ttgtgtgaat cgatagtact aacatacgct ctccgtcaaa acaaaacgaa 4861 acaaaacaaa ctagcaaaat aggctgtccc cagtgcaagt gcaggtgcca gaacatttct 4921 ctatcgatag tcgaccaatt gctcgagttc ggaaataggt caggtacttg ggagattaga 4981 caagcttgct tcatccctga atacaattag gaggaattag acctgcagca agtagcttgg 5041 atttatcagc tttaattgaa ttctatataa tggaagcttg accggagccg ttagttgccc 5101 gtcggacgcg atttgcgagg cgctttatgc gcgcaccggt cgccaccatg atgtcttatc 5161 taaagcaacc gccttacgca gtcaatgggc tgagtctgac cacttcgggt atggacttgc 5221 tgcatccctc cgtgggctac cccgccaccc cccggaaaca gcgaagggag aggacgacat 5281 ttactagggc acagctcgac gttctggaag ctctgtttgc caagacccgg tacccagaca 5341 tcttcatgag ggaagaggtg gcactgaaaa tcaacttgcc agaatccagg gtgcaggtat 5401 ggtttaagaa tcgaagagct aagtgccgcc aacagcagca gcagcagcag aatggaggtc 5461 agaacaaagt gaggcctgcc aagaagaaga gctctccagc tcgggaagtg agttcagaga 5521 gtggaacaag tggccagttc agtcccccct ctagtacctc agtcccaacc attgccagca 5581 gcagtgctcc agtgtctatc tggagcccag cgtccatctc cccactgtct gaccccttgt 5641 ccacttcctc ctcctgcatg cagaggtcct atcccatgac ctatactcag gcttcaggtt 5701 atagtcaagg ctatgctggc tcaacttcct actttggggg catggactgt ggatcttatt 5761 tgacccctat gcatcaccag cttcctggac caggggccac actcagtccc atgggtacca 5821 atgctgttac cagccatctc aatcagtccc cagcttctct ttccacccag ggatatggag 5881 cttcaagctt gggttttaac tcaaccactg attgcttgga ttataaggac caaactgcct 5941 cttggaagct taacttcaat gctgactgct tggattataa agatcagacg tcctcatgga 6001 aattccaggt tttgtgagta aattccggcc cccccccccc ctct //