LOCUS Exported 1942 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION EMMA toolkit Assembly connector L-N. ACCESSION . VERSION . KEYWORDS YCe2718 HC_Kan_conn_L-N SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1942) AUTHORS Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y TITLE EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid Design and Production of Diverse Expression Vectors. JOURNAL ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016. PUBMED 28418644 REFERENCE 2 (bases 1 to 1942) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1942 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 283..312 /label=T3Te terminator /note="phage T3 early transcription terminator" rep_origin 334..921 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 822..841 /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 933..960 /label=T7Te terminator /note="phage T7 early transcription terminator" CDS complement(984..1799) /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind 1707..1726 /label=Kan-R /note="Kanamycin resistance gene, reverse primer" promoter complement(1800..1902) /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" terminator 1903..1934 /label=tonB terminator /note="bidirectional E. coli tonB-P14 transcription terminator" ORIGIN 1 tggaggtcag gtatgattta aatggtcagt attgagcgat atctagagaa ttcgtcagaa 61 gttggtgagt tcagagcgtc tcacgtctat gatgaggatg ttggtggaga gcatgtggag 121 gaagtggata gggaaggttg tagagtagat ccggttgaag tgatgaggat aggaggaggc 181 taccgagacg gtgaatgagg agattgagct aacgaattca agcttgatat cattcaggac 241 gagcctcaga ctccagcgta actggactgc aatcaactca ctggctcacc ttcacgggtg 301 ggcctttctt cggtagaaaa tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 361 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga 421 gctaccaact ctttttccga ggtaactggc ttcagcagag cgcagatacc aaatactgtt 481 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac 541 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc 601 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt 661 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt 721 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc 781 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt 841 tatagtcctg tcgggtttcg ccacctctga cttgagcatc gatttttgtg atgctcgtca 901 ggggggcgga gcctatggaa aaacgccagc aacgcagaaa ggcccacccg aaggtgagcc 961 aggtgattac atttgggccc tcattagaaa aactcatcga gcatcaagtg aaactgcaat 1021 ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga 1081 gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg 1141 actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt 1201 gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagctt atgcatttct 1261 ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcaccaacc 1321 aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc gccgttaaaa 1381 ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag cgcatcaaca 1441 atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt ccctgggatc 1501 gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga 1561 ggcataaatt ccgtcagcca gtttagcctg accatctcat ctgtaacatc attggcaacg 1621 ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata caatcgatag 1681 attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca 1741 tccatgttgg aatttaatcg cggcctcgag caagacgttt cccgttgaat atggctcatt 1801 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt 1861 atttcattat ggtgaaagtt ggaacctctt acgtgccgat caagtcaaaa gcctccggtc 1921 ggaggctttt gactttctgc ta //