LOCUS Exported 1923 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION EMMA toolkit Assembly connector M-R. ACCESSION . VERSION . KEYWORDS YCe3733 HC_Kan_conn_M-R SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1923) AUTHORS Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y TITLE EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid Design and Production of Diverse Expression Vectors. JOURNAL ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016. PUBMED 28418644 REFERENCE 2 (bases 1 to 1923) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1923 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 265..296 /label=tonB terminator /note="bidirectional E. coli tonB-P14 transcription terminator" promoter 297..399 /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" CDS 400..1215 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind complement(473..492) /label=Kan-R /note="Kanamycin resistance gene, reverse primer" terminator 1239..1266 /label=T7Te terminator /note="phage T7 early transcription terminator" rep_origin complement(1278..1865) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1358..1377) /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 1887..1916 /label=T3Te terminator /note="phage T3 early transcription terminator" ORIGIN 1 tgattgcagt ccagttacgc tggagtctga ggctcgtcct gaatgatatc aagcttgaat 61 tcgtttttaa cgcagccgtc tcctcacgac ataatccacc atcaacatgc aagtccagtc 121 aacaactttc cctcagacgt tagtggtaat cgaacctcaa cacctagccc agaacaagcc 181 ggttgagacg aggccgttat gacgaattct ctagatatcg ctcaatactg accatttaaa 241 tcatacctga cctccatagc agaaagtcaa aagcctccga ccggaggctt ttgacttgat 301 cggcacgtaa gaggttccaa ctttcaccat aatgaaataa gatcactacc gggcgtattt 361 tttgagttat cgagattttc aggagctaag gaagctaaaa tgagccatat tcaacgggaa 421 acgtcttgct cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa 481 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgattgta tgggaagccc 541 gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat 601 gagatggtca ggctaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt 661 atccgtactc ctgatgatgc atggttactc accactgcga tcccagggaa aacagcattc 721 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc 781 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacggcga tcgcgtattt 841 cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttggtgcgag tgattttgat 901 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa gcttttgcca 961 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac 1021 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag 1081 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt 1141 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca cttgatgctc 1201 gatgagtttt tctaatgagg gcccaaatgt aatcacctgg ctcaccttcg ggtgggcctt 1261 tctgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga 1321 tgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct 1381 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc 1441 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg 1501 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc 1561 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca 1621 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag 1681 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct 1741 ctgctgaagc cagttacctc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 1801 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 1861 ctcaagaaga tcctttgatt ttctaccgaa gaaaggccca cccgtgaagg tgagccagtg 1921 agt //