LOCUS       Exported                1908 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  part designed to occupy position 6 of EMMA. Functional category: 
            Signal peptide for subcellular targeting.
ACCESSION   .
VERSION     .
KEYWORDS    YCe1885 HC_Kan_Kozak-ATG-MLS_p6
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             87..161
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     terminator      251..282
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        283..385
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             386..1201
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(459..478)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1225..1252
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1264..1851)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1344..1363)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      1873..1902
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 ttgattgcag tccagttacg ctggagtctg aggctcgtcc tgaatgatat caagcttgaa
       61 ttcgttacgt ctcgccagcc gccaccatga gcgtgctgac ccccctgctg ctgcgcggcc
      121 tgaccggcag cgcccgccgc ctgcccgtgc cccgcgccaa gatccacagc ctgggcagcc
      181 gagacggacg aattctctag atatcgctca atactgacca tttaaatcat acctgacctc
      241 catagcagaa agtcaaaagc ctccgaccgg aggcttttga cttgatcggc acgtaagagg
      301 ttccaacttt caccataatg aaataagatc actaccgggc gtattttttg agttatcgag
      361 attttcagga gctaaggaag ctaaaatgag ccatattcaa cgggaaacgt cttgctcgag
      421 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa
      481 tgtcgggcaa tcaggtgcga caatctatcg attgtatggg aagcccgatg cgccagagtt
      541 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcaggct
      601 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga
      661 tgatgcatgg ttactcacca ctgcgatccc agggaaaaca gcattccagg tattagaaga
      721 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca
      781 ttcgattcct gtttgtaatt gtccttttaa cggcgatcgc gtatttcgtc tcgctcaggc
      841 gcaatcacga atgaataacg gtttggttgg tgcgagtgat tttgatgacg agcgtaatgg
      901 ctggcctgtt gaacaagtct ggaaagaaat gcataagctt ttgccattct caccggattc
      961 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat
     1021 aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct
     1081 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg
     1141 tattgataat cctgatatga ataaattgca gtttcacttg atgctcgatg agtttttcta
     1201 atgagggccc aaatgtaatc acctggctca ccttcgggtg ggcctttctg cgttgctggc
     1261 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgatgct caagtcagag
     1321 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     1381 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     1441 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     1501 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     1561 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     1621 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     1681 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     1741 tacctcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     1801 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     1861 ttgattttct accgaagaaa ggcccacccg tgaaggtgag ccagtgag
//
