LOCUS       Exported                1943 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  part designed to occupy position 11 of EMMA. Functional category: 
            polyadenylation signal sequence .
ACCESSION   .
VERSION     .
KEYWORDS    YCe1889 HC_Kan_SV40-polyA_p11
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y
  TITLE     EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid
            Design and Production of Diverse Expression Vectors.
  JOURNAL   ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016.
  PUBMED    28418644
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     polyA_signal    66..187
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(103..122)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     157..176
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     terminator      283..312
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      334..921
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     822..841
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      933..960
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(984..1799)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1707..1726
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(1800..1902)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      1903..1934
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
ORIGIN
        1 ggaggtcagg tatgatttaa atggtcagta ttgagcgata tctagagaat tcgtacgtct
       61 cgggtaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
      121 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
      181 tatcttatca tgtctgtcgt ccgagacgaa aacgaattca agcttgatat cattcaggac
      241 gagcctcaga ctccagcgta actggactgc aatcaactca ctggctcacc ttcacgggtg
      301 ggcctttctt cggtagaaaa tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
      361 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
      421 gctaccaact ctttttccga ggtaactggc ttcagcagag cgcagatacc aaatactgtt
      481 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
      541 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
      601 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
      661 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
      721 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
      781 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
      841 tatagtcctg tcgggtttcg ccacctctga cttgagcatc gatttttgtg atgctcgtca
      901 ggggggcgga gcctatggaa aaacgccagc aacgcagaaa ggcccacccg aaggtgagcc
      961 aggtgattac atttgggccc tcattagaaa aactcatcga gcatcaagtg aaactgcaat
     1021 ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga
     1081 gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg
     1141 actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt
     1201 gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagctt atgcatttct
     1261 ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcaccaacc
     1321 aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc gccgttaaaa
     1381 ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag cgcatcaaca
     1441 atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt ccctgggatc
     1501 gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga
     1561 ggcataaatt ccgtcagcca gtttagcctg accatctcat ctgtaacatc attggcaacg
     1621 ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata caatcgatag
     1681 attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca
     1741 tccatgttgg aatttaatcg cggcctcgag caagacgttt cccgttgaat atggctcatt
     1801 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
     1861 atttcattat ggtgaaagtt ggaacctctt acgtgccgat caagtcaaaa gcctccggtc
     1921 ggaggctttt gactttctgc tat
//
