LOCUS Exported 2415 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION part designed to occupy position 9 of EMMA. Functional category: Selection marker. ACCESSION . VERSION . KEYWORDS YCe1899 HC_Kan_PuroR_p9 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2415) AUTHORS Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y TITLE EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid Design and Production of Diverse Expression Vectors. JOURNAL ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016. PUBMED 28418644 REFERENCE 2 (bases 1 to 2415) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2415 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 74..670 /codon_start=1 /gene="pac from Streptomyces" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA PRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA" primer_bind 567..587 /label=Puro-F /note="Puromycin resistance gene, forward primer" terminator 759..788 /label=T3Te terminator /note="phage T3 early transcription terminator" rep_origin 810..1397 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1298..1317 /label=pBR322ori-F /note="pBR322 origin, forward primer" terminator 1409..1436 /label=T7Te terminator /note="phage T7 early transcription terminator" CDS complement(1460..2275) /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind 2183..2202 /label=Kan-R /note="Kanamycin resistance gene, reverse primer" promoter complement(2276..2378) /label=cat promoter /note="promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase" terminator 2379..2410 /label=tonB terminator /note="bidirectional E. coli tonB-P14 transcription terminator" ORIGIN 1 ctatggaggt caggtatgat ttaaatggtc agtattgagc gatatctaga gaattcgtca 61 cgtctcggcg tccaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc 121 cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt 181 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt 241 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac 301 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga 361 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg 421 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtg tcgcccgacc accagggcaa 481 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc 541 cgccttcctg gaaacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac 601 cgtcaccgcc gacgtcgagt gcccgaagga ccgcgcgacc tggtgcatga cccgcaagcc 661 cggtgcctaa taatgctcga gacgaaaacg aattcaagct tgatatcatt caggacgagc 721 ctcagactcc agcgtaactg gactgcaatc aactcactgg ctcaccttca cgggtgggcc 781 tttcttcggt agaaaatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 841 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta 901 ccaactcttt ttccgaggta actggcttca gcagagcgca gataccaaat actgttcttc 961 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg 1021 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt 1081 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt 1141 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc 1201 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca 1261 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata 1321 gtcctgtcgg gtttcgccac ctctgacttg agcatcgatt tttgtgatgc tcgtcagggg 1381 ggcggagcct atggaaaaac gccagcaacg cagaaaggcc cacccgaagg tgagccaggt 1441 gattacattt gggccctcat tagaaaaact catcgagcat caagtgaaac tgcaatttat 1501 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa 1561 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc 1621 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga 1681 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagcttatgc atttctttcc 1741 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca ccaaccaaac 1801 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgccg ttaaaaggac 1861 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat 1921 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttccct gggatcgcag 1981 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca 2041 taaattccgt cagccagttt agcctgacca tctcatctgt aacatcattg gcaacgctac 2101 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg 2161 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca 2221 tgttggaatt taatcgcggc ctcgagcaag acgtttcccg ttgaatatgg ctcattttag 2281 cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt 2341 cattatggtg aaagttggaa cctcttacgt gccgatcaag tcaaaagcct ccggtcggag 2401 gcttttgact ttctg //